EMAGE mouse gene expression spatial database

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EMAGE:7438

Zfp658 zinc finger protein 658 ( MGI:2652821)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004582. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004582_01	euxassay_004582_02	euxassay_004582_03	euxassay_004582_04

euxassay_004582_05	euxassay_004582_06	euxassay_004582_07	euxassay_004582_08	euxassay_004582_09

euxassay_004582_10	euxassay_004582_11	euxassay_004582_12	euxassay_004582_13	euxassay_004582_14

euxassay_004582_15	euxassay_004582_16	euxassay_004582_17	euxassay_004582_18	euxassay_004582_19

euxassay_004582_20	euxassay_004582_21	euxassay_004582_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7438_wholemount_strong.wlz

7438_wholemount_moderate.wlz

7438_wholemount_weak.wlz

7438_wholemount_possible.wlz

7438_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7438_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7657
Entity Detected:	Zfp658, zinc finger protein 658 ( MGI:2652821)
Sequence:	sense strand is shown >T7657 AAGAGGTAATCCGCTGGGGGATTTTTCTACCTCACCACAGCTGGCCTCTCTGCTCAGCTGCTCTCCTGGG AACCTCTAGCCACTCAACCAGCTTCCAGTTCCTGAGAAAAATTGCATCTGCCAGCAAAGAGGCAAGAGAG GAAACATACCTGCTGACATGAAATTGTCCCAGAAGGCCCAGAGACTGCACCCACAGGAGCAGGAAGGGTC CCGTGAGAGATTGGTGTCCTTTGAGGATGTGACTGTGGACTTCAGCCAGGAGGAGTGGCGACGCCTGGAC TCCGTCCAGAGACACCTGTACCAGGACGTGATGCTGGAGATCTACAGCCACCTCTTGGCAGTGGGCTACC CTGTTCCTAGCCCTGGGGCCATCTTCAGGATAAAGAAAAGAAAGGAGGCACGGACAGGAGAGGCCAAGCT CCCACATCCTTGTTGGTGTCAAGAAGTGGAATCAGAGTTTTACAGGCCTCAGCAGAAAGCATCTGAGGAG GCAGCATTCCAAACTAGTAAGGCAAGCGCAGTCACAAGGCATGGCTTGTGGTGCTCGATTTTAGAAGAAC TGTGGCAAGATCCTGACCCTGCACAAAGAGATGGGCAAAACCAGATCCCACCCTGGAGTCCTGGGGCTTC CCTCAACAAGAAAACAGCGCGAGCAGATAGAGACTGTGGGTGGAAAGAGCCCGGGAAGACCACTCCCTTC GTAGCTAACCTTTTTTCTACACAAAAGGGAGCTCCAAGGTTACTTGCAAAACGTTTGAACCCTAACCTTG AAGCCAACGGTGAAGAGCAAGGCAGTGTCACAAAACAGCTGAATGGTCTTGTTACATCTGCTCACCTCCT CCCGCAAGACTCTTCTAATGCTAACTGTACAATTCTTCGTGAAACATGGAATCCGTGCAAAGGTAACCAG TGCAGAAAAGTTCCCACCCATAAACAACTGACAGAGAAAGAAGCTCATTCTCGTGAGAAACTAGATGAAG GCACGGAGTGTAGAAAAGTCTTCTATGACGAATCTGCTTTCTGTAAACACCAGGCAGCTCACGCTGGAGA AGGACCTTTTGTCTGTCACAAGTGTGGGGAAGCCTTCCTCCAGAAGACAGAGTTTGCCTCCCATCAAGAA ACTCACCCAGAGAAACCTTATGAGTGTCCCGACTGCGGGAAGTCATTTAGTCGAACGTCCAACCTGCAGG TCCACCATCGAATTCACACTGGAGAGAAACCTTATGAGTGTCGGGACTGCGGCAAATCATTCAATAATAC ATCCCAGCTGAAGGTACATTATCGGATACATACAGGGGAGAGACCTTACGTGTGTCCCGTATGTGGGAAG GCCTTCAAGCAGAAATCAATCCTCAGCACGCATGAGACAATTCACACTGGGGAGAAGCCTTACAAATGTA CCGTTTGTGGGAAACTGTTCAGCTGTACGTCCCGACTGAAAGTGCACTACCAGATTCATATGAAGGAGAA ACCTTATGAATGCGGTGACTGTGGAAAAGCCTTCAAGCGAAAGTCAAGCCTCACCGTTCACCAGAAGATC CATATCAGACAAACACACCACGAGTGCAGCGAGTGTGGGAAAACGTTCAACCAGAAGTCCGAGCTCAGCA CTCACCAGAGACTTCACCTGGGCAAGAATTCCCACAGATGCAGCGACTGTGGGAAATCGTTCACTTACGC ATCCCAACTGAAGATGCACCGTCGGGTTCACACGGGGGAGAAGCCCTACAAGTGCCGGGACTGTGGGAAG TCCTTTACGTACTCATTCACGCTGAATGTGCATCGCCGAATTCACAAGGTGGAGAAGCCTCACAAGTGCA GCATCTGTGGGAAAGCTTTGGCGTCTAAGTACCAACTGGAAGAGCATGAGCGAATTCACACAGGAGAGAA GCCTTACGTGTGTACTGAATGTGGAAAAGGCTTCCATGGTAGGTCAGGTTTCCTAAGACATCAGATTACT CACACGAGGGATAAACCTTTCGTCTGTCATAAATGTGGGAAGGCCTTCTTTCAGAGGTCACAGCTGACAT CTCATCAGCAGACTCACACAGGAGAGAAGCCTTATACGTGCCATTACTGCGGGAAATCATTCAGTCACAC ATCCCAGCTGACGGTCCATCATCGAATTCACACAGGGGAGAGACCTTACAAATGCGACTATTGTGCGAAG TCGTTCAGCAACTCCTCCCAACTGAAAGAGCATCTCCGCATTCATACCGGGGAGACGCCTTATGCATGTT CCGAATGTGGGAAGGCCTTCAGCCGACGATCGTCCCTCAATCTACACACGAAAATTCATACTGGAGAAAA ACACCATATATGTAGTGAGTGTGGGAAAGCCTTTAGTCAGAAGTCAGTACTGCGGACACATCAGAGAATA CACACTGGGGAAAAGCCTTACAAATGCAGTGACTGTGGGAAGGCCCTGGCTTCAAAGGGCCAACTTCGAG ATCATCAGCGGATTCACACTGGCGAGAAACCCTACATGTGCCCTGAATGTGGGAAGGGTTTCTTTGGCAG GTCGTCTTTGCATAGACATCAGATAACTCACACTAAGGAGAGACCTTTTGTCTGTCAAAAATGCGGGAAG ACCTTCATCCAGAAGTCAGCACTGACGTCCCATCAGCAAATTCACACAGGCGAGAAGCCCTATGTGTGCC CTGAGTGTGGGAAGGGCTTCTACAATAAGTCATCTTTACCTAGGCATCAGATGACTCACACTAGGGGGAG ATCTTTTGTCTGTCAAAAATGTGGGAAGGCATTCCTCCAGAAGTCTGTGTTGAAATGCCATCAGCGGACT CACACTTGCAAGAAGCCTTAGCAATGCCATAGGAAATTGTTCCATAATAAAACCCACTGAGGGGCCCTCA TCATCCCTACATAGAAATGCAGAGAGCATCCCAACTGGATGTGCAGCACTGGGTCTAGAATGGAGAGAGA CACTACTGTGTTCTGAGTGTTAAAAGGCTTTTAACAGCATGTCAGATGATAATAAATAGCAAGCAGCTCA TAGTAGACACAGTTCTTTTTAGGCTCAGTTTTTGTGTGAAGCATATTTCCTCATAGTCTATAAACATATT CCTAACTGAGGTATAATCTTTAAATTACTTGAAGAGAATGTTTTAAAAGAATTTATTGGGGCTGGAGAGA TGGCTTAAGAGCGCTGGCTGCTCTCCCAGAGGTCCTGAGTTCAATTCCCAACAACTACATGGTGGCTCAC AACCGTCTATAATGAGATCCGATGCCCTCTTCTGGTGTGTCTGAAAGCAGCGACAGTGTACTCACATACA TGAAATAAATCTTTTTTTTTAAAGGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30742836 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30742836 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7442 same embryo
	EMAGE:7440 same embryo
	EMAGE:7439 same embryo
	EMAGE:7437 same embryo
	EMAGE:7441 same embryo
	EurExpress:euxassay_004582 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source