EMAGE mouse gene expression spatial database

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EMAGE:7439

Arcn1 archain 1 ( MGI:2387591)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004580. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004580_01	euxassay_004580_02	euxassay_004580_03	euxassay_004580_04

euxassay_004580_05	euxassay_004580_06	euxassay_004580_07	euxassay_004580_08	euxassay_004580_09

euxassay_004580_10	euxassay_004580_11	euxassay_004580_12	euxassay_004580_13	euxassay_004580_14

euxassay_004580_15	euxassay_004580_16	euxassay_004580_17	euxassay_004580_18	euxassay_004580_19

euxassay_004580_20	euxassay_004580_21	euxassay_004580_22	euxassay_004580_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7439_wholemount_strong.wlz

7439_wholemount_moderate.wlz

7439_wholemount_weak.wlz

7439_wholemount_possible.wlz

7439_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7439_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7651
Entity Detected:	Arcn1, archain 1 ( MGI:2387591)
Sequence:	sense strand is shown >T7651 AAGAGGTAATCCGAGGCTCCTGTCAGGGGCGGCAGGTCCGGAGCGGCCAGAGCTCCGCGTCTCTGGGCAG CAGCCCGAGTCCCCGCAGAGCCAGAGCGCGGCGCAGCGCGCCCCACCTGCGGCCTCACCATGGTGCTGTT GGCAGCAGCAGTCTGCACGAAAGCGGGAAAGGCTATTGTTTCTCGACAGTTCGTGGAGATGACCCGAACT CGGATTGAAGGCTTATTAGCAGCTTTCCCAAAACTCATGAACACTGGAAAACAACATACTTTTGTTGAAA CAGAGAGTGTAAGATATGTGTACCAACCTATGGAGAAACTGTACATGGTATTGATCACTACAAAGAACAG CAATATCTTAGAAGATTTGGAGACTTTAAGGCTCTTCTCAAGGGTGATCCCTGAATATTGCCGAGCCTTA GAGGAAAATGAAATATCTGAGCACTGTTTTGATTTGATTTTTGCTTTTGATGAAATTGTTGCCCTGGGAT ACCGGGAGAATGTTAACCTGGCACAGATCAGAACCTTTACAGAAATGGACTCTCACGAGGAGAAAGTATT CCGAGCAGTCAGAGAGACTCAAGAACGTGAGGCTAAGGCTGAGATGCGGCGTAAAGCAAAGGAATTACAA CAGGCCCGAAGAGATGCAGAGAGACAGGGGAAAAAAGCACCAGGATTTGGCGGATTTGGTAGTTCTGCAG TGTCTGGAGGCAGCACAGCAGCCATGATCACAGAGACTATCATTGAAACTGATAAACCAAAAGTGGCGCC TGCACCAGCCAGACCTTCAGGCCCCAGCAAGGCTTTGAAACTTGGAGCTAAAGGAAAGGAAGTAGATAAC TTTGTGGACAAATTGAAATCTGAAGGTGAAACTATTATGTCTTCTAATATGGGAAAACGTACCTCAGAAG CAACCAAAGTGCATGCTCCACCTATTAATATGGAAAGTGTGCACATGAAGATTGAAGAGAAGATCACACT AACCTGTGGGAGAGATGGAGGATTACAGAATATGGAGTTGCATGGCATGACCATGCTTAGGATCTCAGAT GACAAATTTGGCCGGATTCGTCTTCATGTAGAAAATGAAGATAAGAAAGGGGTGCAGCTACAGACGCATC CAAATGTGGATAAAAAACTTTTTACTGCAGAGTCTCTCATTGGCTTGAAAAACCCAGAGAAGTCATTTCC AGTCAACAGTGATGTAGGGGTACTAAAGTGGAGACTACAAACAACAGAGGAATCTTTTATTCCGTTGACA ATTAATTGCTGGCCTTCGGAAAGTGGAAATGGCTGTGATGTCAACATAGAGTACGAACTACAGGAAGATA ATTTAGAGCTCAATGATGTGGTCATCACTATCCCACTCCCATCGGGTGTTGGCGCGCCTGTGATTGGTGA GATCGATGGGGAATATCGACATGACAGTCGGAGAAATACGTTGGAGTGGTGCCTGCCAGTGATTGATGCC AAAAATAAGAGTGGCAGCCTGGAGTTCAGCATTCCTGGGCAGCCTAATGACTTCTTCCCTGTTCAAGTTT CTTTCATCTCCAAAAAGAATTACTGCAACATACAGGTTACCAAAGTGACCCAGGTGGATGGAAACAGCCC CGTCAGGTTTTCCACGGAGACCACTTTCTTAGTGGACAAATACGAAATCCTGTAATACCAAGAAGGGAAA GCTGAAAAGGAAGGATTTCAGATTAATAAAGAAGAAGCCAAAAGTGGCTGAAGAGTTTTCCAAATTTACA AGCCATTGGAGGTCCCCCAACCCCCTTTTCTGATACAGTGCACGTTCTCTGCGCGCAAGGACCCTCAACT CACACCCAGTGTTGCGGTGTCACAGAGACATTTTTGACAAATGACACTGACATAAAGGGAAAGGCTGCTG ATTTCTTTGGCCAGCAGGAAGCCAGCTCTCCAGGGAATGGAATGCCCCAGTAAATAGCTCTTCTGCCTTC ACCTAAGTCTTCCTTTATTTTAAGCCCTAAATATAGGTATATTTCATACTTTGTTTTAAAAAGTTTTCTC TATGGATTTGAGTCTTTCACACCCCTTTGCTTTCTGTTTTCTTTCAGTACACTTAGTCACACACTGGGGT CATCTGTCTGTTGTACTGTGTTCAGCACTCTGAGAACAAGGTTATCTAACCCATTCTTGACATTAGACAG TACCAGTGAACTGCTTAAGGGATGTAGAGTCTAGAAGTTGAAATACAAGGTTGTCTTGTTTTCTCTCCTG TATCCATCCATTGCTCCATCTCTTTATGAAGTCGTCCTTGTGAGCCCTGCCCTTCCAGTTGCAGGCAGTG TTGTGAATCCCATTTGTGTCAATATTAAAGGTAGCCAGCTGGGAAGCAGCTTTTATGTGTACACACCTTT TCAAGATGCCGAATAAGATGGCTACATCCATTTCCACAGGAAATCATGTCTGCTGCTAATGCTACCCCAA CTTACAGTTCCCTACTTGACTTATGGTTCCCTACGTAACTTACAGTTCTCTCCGTGCTGTGGGGCGATTG AGTTGAAGCACGTAGTCCACTCAGATTCCGCGTGTCCACCTCCTCCCTTCCCAGATGCTTTTCCCTTCAT CAGTCTTCTTTCCAGCAGAAATGAGTCTAGTGAGTCTGTGCCATTTCTCATTAGCCCTAAATCACTGGAG AGATCCATCTTTACCATTTCAGTGGTAGCATTTTGAGAGGTGTATAGTGAGGGGTTACTCAAATATACCC CATCTAACCTGACATTTACCACCTCCCTCTCTGTCCTTGTTTCTGAAAACCTTTTTTTCTCCTCCTCAGG CTTTTTGGTGTCATCATGTAATAATTGTAGGAGTTCTCAGCTCATTTTGATCTATTACCTTTTCAATAGA GGCTGAATCAGCCAATCCCACCCGCCAAGCACTGAACTGGGCGGGATGGACGATCAGTTGCTGAAGGCTG CAAGCAGTAGCAATGGGAAACAGTAGATGACCTCCATTCTCTCAGGTGGCTGCAGAGCAGAGCAGAGCAG AGATCTCCCTCTGAACACCTGCCTTCCCTGGCAAGAGTATACTATCCATGAAGCAGGGCAGTCCCACAGA AGACTGTGGCTGGGATGGCTTGCCAAAGAAATTTCTAGCCTTTCCCTTTCCCCTAAACTGCATCAGGGAA GAATCCTTATCTCTAGCTTCGTTTTCACATGAGGTTTTCTGAGGAAGGGGACTGGGATAGGAAGTCTGTC ATGTAGTAAAGTAGACGAGAAATCTTATTAATCCAGTTGTATTTCAGAGTTTGTCCCTATGATTTTTAAA GTCAAGTTTAATTTCACAAGACTTTTTTTCTGAAATTACTTTTGGGGTAATATTTAAAATGAGAGACATT TTGTAACCCTGTAAAATACATAGGGAATATAACATTCCAATGTACACAACGAAGGCAAATTCTTAATCAA ATAAAAGAGTATTATAAAATGAGATGTTTACTGGACTCTAACTTTGAAGTCTGCTGGTGATTCATGATAC TGTAATAGGATCTAAAATTAAAGATTAATGATATGTTTTTTTTTTAAAGAAAATAATCTATCCAAGTGTA TTCATTGCCTTGTCTCTGATTCCCAGGTATTGTGCCTCTGTTGTGTGTTTGAGTACACACACTCAATCCC GAAAGCAGAGGTTTTTGGTAGAATTAGGCTTCTTGGAGCTTTTGCCAGGTCTGGAGTATTCCAATTTTTA GCAGTTTAGAGTCAGTCAAATGAAGCATTTTTTTAAAGGATATCATTCTGCAGTATCCTGAAGCCTGCCC TTTGTCAGACAGTGATGAGTAAATGAATAGGGATGGGGGATGCTTTAGAAATGTGCTTATCGGGCTGGTG AGATGGCTCAGTGGGTCTTCCGAAGGTCCGAAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCAT CCGTAATGAGATCTGACTCCCTCTTCTGGAGGGTCTGAAGACAGCTACAGTGTACTTACATGTAATAAAT AAATAAATAAATAAATCTTTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30461105 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30461105 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7442 same embryo
	EMAGE:7440 same embryo
	EMAGE:7438 same embryo
	EMAGE:7437 same embryo
	EMAGE:7441 same embryo
	EurExpress:euxassay_004580 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source