EMAGE mouse gene expression spatial database

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EMAGE:7482

Cyp17a1 cytochrome P450, family 17, subfamily a, polypeptide 1 ( MGI:88586)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004607. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004607_01	euxassay_004607_02	euxassay_004607_03	euxassay_004607_04

euxassay_004607_05	euxassay_004607_06	euxassay_004607_07	euxassay_004607_08	euxassay_004607_09

euxassay_004607_10	euxassay_004607_11	euxassay_004607_12	euxassay_004607_13	euxassay_004607_14

euxassay_004607_15	euxassay_004607_16	euxassay_004607_17	euxassay_004607_18	euxassay_004607_19

euxassay_004607_20	euxassay_004607_21	euxassay_004607_22	euxassay_004607_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7482_wholemount_strong.wlz

7482_wholemount_moderate.wlz

7482_wholemount_weak.wlz

7482_wholemount_possible.wlz

7482_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7482_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal cortex

strong

regional

strong expression: see section 05 06 07 08 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7620
Entity Detected:	Cyp17a1, cytochrome P450, family 17, subfamily a, polypeptide 1 ( MGI:88586)
Sequence:	sense strand is shown >T7620 CTTCTGCAGGCCAAGAGATAACACGTCTTCAAGGTGACAATCAGAAACGCCTTTTAAAAGTTCTCCTTCT CTGCTGAAATTGCTGTAGCTTCTCCACTCCACAGCTGGCCATCTGCCTACACCTGGCTGCCATGTGGGAA CTTGTGGGTCTCTTGCTGCTCATCCTGGCCTATTTCTTTTGGCCCAAGTCAAAGACACCTAATGCCAAGT TCCCCAGGAGCCTTCCATTCCTGCCCCTGGTGGGTAGTCTACCGTTTCTCCCCAGACGTGGTCATATGCA TGCCAACTTCTTCAAGCTGCAGGAAAAGTATGGTCCCATCTATTCTCTTCGCCTGGGTACCACAACTGCA GTGATTGTCGGTCACTATCAGCTGGCCAGAGAAGTGCTCGTGAAGAAGGGGAAAGAATTCTCTGGTCGGC CCCAGATGGTGACTCTAGGCCTCTTGTCGGACCAAGGAAAAGGCGTCGCCTTTGCGGATAGTAGTAGCTC CTGGCAGCTGCACCGGAAGCTGGTATTCAGCACCTTTTCCCTGTTCAGGGATGACCAGAAACTGGAGAAG ATGATATGTCAGGAAGCCAACTCACTGTGTGACTTGATACTTACATACGACGGGGAGTCCCGAGATCTGT CTACGCTCATCTTCAAGTCAGTAATCAATATCATCTGTACCATCTGCTTCAACATCTCTTTTGAGAACAA GGATCCGATACTGACTACCATACAGACCTTTACAGAGGGTATTGTGGATGTCCTGGGCCACAGCGATCTG GTGGACATATTCCCGTGGTTGAAGATTTTTCCCAATAAAAACTTGGAAATGATAAAGGAACACACTAAAA TTCGAGAAAAAACACTGGTTGAAATGTTTGAAAAATGCAAGGAGAAATTCAATAGTGAATCTCTCTCCAG CCTGACAGACATTCTGATACAAGCCAAGATGAATGCAGAAAATAATAACACTGGGGAAGGCCAGGACCCA AGTGTGTTCTCAGATAAGCATATCCTTGTCACGGTGGGAGACATCTTTGGGGCAGGCATAGAGACAACTA GCTCTGTGCTGAGCTGGATCCTGGCTTTCCTGGTGCACAATCCTGAGGTGAAGAGGAAGATCCAAAAGGA GATTGACCAGTATGTAGGCTTCAGTCGAACACCGTCTTTCAATGACCGGACTCACCTCCTCATGCTGGAG GCCACTATCCGAGAAGTGCTTCGTATCAGGCCGGTGGCCCCCTTGCTCATCCCACACAAGGCTAACATTG ACTCCAGCATTGGAGAGTTTGCCATCCCGAAGGACACACATGTGATCATCAATCTCTGGGCACTGCATCA CGATAAAAATGAATGGGACCAGCCAGATCGGTTTATGCCTGAGCGCTTCTTAGATCCAACAGGAAGCCAT CTCATTACACCCACACCCAGTTATTTGCCCTTCGGAGCTGGTCCCCGATCGTGCATTGGAGAGGCTCTGG CCCGGCAGGAGCTCTTTATCTTCATGGCCTTGCTGCTGCAGAGGTTTGACTTTGATGTGTCAGATGACAA ACAGCTGCCCTGTCTGGTGGGTGACCCCAAGGTGGTCTTTCTGATCGACCCTTTCAAAGTGAAAATCACA GTGCGACAAGCATGGAAGGATGCACAGGTTGAGGTTAGCACCTAGAGGCCGAATCTAACGTCCCGGATCC CATGCCTTGACACCCACAGCCCAATCTTAGAGGTGCCTCCAACAATCCTCCTCACTCCTATCCCGTTTTC TACTTGGCAGCAATGAAGGGTGAGACACATATTAAAGGTTTTCCAATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30436278 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30436278 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7487 same embryo
	EMAGE:7485 same embryo
	EMAGE:7486 same embryo
	EMAGE:7484 same embryo
	EMAGE:7483 same embryo
	EurExpress:euxassay_004607 same experiment
	MGI:4824158 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source