EMAGE mouse gene expression spatial database

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EMAGE:7483

Zcwpw1 zinc finger, CW type with PWWP domain 1 ( MGI:2685899)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004634. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004634_01	euxassay_004634_02	euxassay_004634_03	euxassay_004634_04

euxassay_004634_05	euxassay_004634_06	euxassay_004634_07	euxassay_004634_08	euxassay_004634_09

euxassay_004634_10	euxassay_004634_11	euxassay_004634_12	euxassay_004634_13	euxassay_004634_14

euxassay_004634_15	euxassay_004634_16	euxassay_004634_17	euxassay_004634_18	euxassay_004634_19

euxassay_004634_20	euxassay_004634_21	euxassay_004634_22	euxassay_004634_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7483_wholemount_strong.wlz

7483_wholemount_moderate.wlz

7483_wholemount_weak.wlz

7483_wholemount_possible.wlz

7483_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7483_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7617
Entity Detected:	Zcwpw1, zinc finger, CW type with PWWP domain 1 ( MGI:2685899)
Sequence:	sense strand is shown >T7617 GGAAGGGATGTGCGGAATGGCTGCGACAGCGTTAGCTGCGCTTCACCTTTAGGTCCTCGTCGCCCCAGAA GGCACAGAAAGTATAGTTTCTGAACTGAATGATGGCAGCATTACAGACTCACAAAGAATACGAAAAGGGG ACAAAGAAAACCTTTGCTCCACCTACACAAAAATTACATAGCGAGAAACCTCAGCCCAGCTCATGGAAGG AGGACGCCCCAGGGACCAGTTCTCCAGAGGCAGAGACCAAGCCAAGCCTGCTCAAAGCCAGCTTAAAGAA GGAGCAGAAACCAACCACAGAGCATGGTCCAAACAGGGGCCAGGAGAGAAAGCTAAAGGCTCAGGACCAG CCAGCCAAGAAGAAAGGAAAGGAAAGAACTCTTACCAGTGCAGAATTTGAGGAGATTTTCCAGATTGTAC TACAGAAGTCCCTGCAGGAGTGCTTGGAGACTTCTTCCTGTGTCCAACACATCAGACCTACCAAATTGGA TGAAGAACCGGGCATTGTTCCTCCTGCAACTGATAAGAAAGATGCTGATCCAGAGAAGGTAATAACTCCT GATACTCCAAAGATTGCATCTTCTTTAGAAGAGGAAGTGAATTCTGAGATGGGGACATCTAAGCTAGGCC AGCCAGTTACTGAACCCTCTAAGAAGAAATTTAATAGACTCTCTTTAAGCAAACAAAAGAAGAAAGCTGA AGATGAGAAAATGGAGAAGATCCAAGATGGCCGTGAGTGCAGTCTGAAGGAGAAACAGAAGATAGTGATT CAGGATCAGTCTCAGATCAGAGGTCCCCAGAAGGAAGAGGAGAGTGGTTTTGGCCACTGTGTCATCTGGG TTCAGTGTTCCTCTCCAAAGTGTGAGAAGTGGCGGCAGCTACGTGGAAACATTGATCCCTCAGTTCTTCC AGACGACTGGTCTTGTGACCAGAATCCAGATCCGAATTATAATCGCTGTGACATTCCTGAGGAGAGCTGG GCAGGGTGCGAGAGTGATGTGGCCTATGCCTCCTATGTCCCAGGATCCATCATCTGGGCCAAGCAATATG GCTACCCATGGTGGCCAGGCATGATAGAAGCTGATCCTGACCTGGGGGAGTACTTTCTGTTTGCTTCTCA TCTTGATTCCCTGCCGTCTAAGTACCACGTGACATTTTTTGGAGAAACGGTTTCTCGTGCATGGATCCCA GTCCGCATGTTAAAGAACTTCCAGGAGCTGTCTTTGGAGCTAGTGAAAAAATGCAAGAACAAGAACCCTA ATCAGAAGCTGGAGGCAGCCATAGCGATGGCTCACAGGGCAGAACAGACCAGTATTCAGGAGCGGGTTAA CTTGTTTGGTTTCTGGAGTCGATACAATGGAGCTGACATCAGTGAGGAAGGGGAAGATTTGACTCTCTGT GAGTCCAACAACCCTGAGTCTTGTCTGGAGAAGGAAGAGAAGGACTTAGAGGAGGAGAAGGAGGAGGAAG AAGAGAAGAAAGATCCAACTTTGCCTAGACCCAAGCCAGCTAAAATGCAGACAAAAAAGCCCAAGTCAAG AGGCCCAGCTGGAGGACCAGATGGGACTCCAAAGAAGAAGACTGCGAAGAAATCTCTGGTCAGTGAATCC ACAGTCCCTCCTGTACCCACACTGGGAGGGAAGGAAGAGCAGGGGAACTCAGACCTGGACCATCCAGTCC CTAAGAAAAAATTTAAAGCTCCTGAGAACAAGACTTCAGCCACCAACTTATCTGAGGAGAAAGAAATTAA AATTGTGTCCAAATGCCCTACTCCATCAGCTCAGCATGGGGCTTGTCCATTGGGAAAGGAAGGGCTTGTG CCCCACATGCCCCCGACACAGGAGGCAGCAAGTTTCCCTCCTGACGATGATTGCTCCAGTGACCTGGACT TGGAGCAACTCATGGAAGACATTGGAGAGCCAGAAGAGAGAGGGGAGATGCAGCAGAGAGGCAGCTCAGA GGAGTTCCTGGCGGCCCTCTTTGAGGAGTAGAGTATTCTGGTCCCCCTGCTGTTGTTTGCCTGCAGGGAG AGCCTCTCATTTCTTCCCGAGCAGCTGATAAATGTTGGGGTTGTTGACCTGCTCTGTTGTTCAAGTCAGC TGTGCAGCCTGGGTTAATAAAGCAGGTCTCTGGAATTACTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30107853 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30107853 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7482 same embryo
	EMAGE:7487 same embryo
	EMAGE:7485 same embryo
	EMAGE:7486 same embryo
	EMAGE:7484 same embryo
	EurExpress:euxassay_004634 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source