EMAGE mouse gene expression spatial database

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EMAGE:7491

Rgl1 ral guanine nucleotide dissociation stimulator,-like 1 ( MGI:107484)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004442. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004442_01	euxassay_004442_02	euxassay_004442_03	euxassay_004442_04

euxassay_004442_05	euxassay_004442_06	euxassay_004442_07	euxassay_004442_08	euxassay_004442_09

euxassay_004442_10	euxassay_004442_11	euxassay_004442_12	euxassay_004442_13	euxassay_004442_14

euxassay_004442_15	euxassay_004442_16	euxassay_004442_17	euxassay_004442_18	euxassay_004442_19

euxassay_004442_20	euxassay_004442_21	euxassay_004442_22	euxassay_004442_23	euxassay_004442_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7491_wholemount_strong.wlz

7491_wholemount_moderate.wlz

7491_wholemount_weak.wlz

7491_wholemount_possible.wlz

7491_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7491_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7558
Entity Detected:	Rgl1, ral guanine nucleotide dissociation stimulator,-like 1 ( MGI:107484)
Sequence:	sense strand is shown >T7558 GTGGGAAGCGCGGGGACCCGGAGCCGGGCCAGAGAGACGCCCCGACCGCCTCGGAGCAGGGCGCACCATG CAGCGTCCGTGTGCCGGAAAGAAAACTGAGAATGAAATTACTTTGGCAAGCTAAAATGAGCTCGATTCAG GACTGGGGTGAAGAGGTAGAGGAAGGAGCTGTTTACCATGTCACCCTCAAAAGAGTCCAGATTCAACAGG CGGCCAATAAAGGAGCGAGATGGCTAGGGGTTGAAGGGGACCAGCTGCCTCCAGGACACACAGTCAGTCA GTACGAGACCTGCAAGATCAGGACCATCAAAGCTGGTACGCTGGAGAAGCTTGTGGAGAACCTGCTGACG GCTTTTGGGGACAATGACTTTACCTACATCAGCATCTTTTTGTCGACATACAGAGGCTTTGCCTCGACTA AGGAAGTGCTGGAGCTGCTGCTGGACAGGTATGGAAACCTGACAGGCCCAAACTGTGAAGACGATGGAAG CCAAAGTTCACCCGAGTCCAAGGCCGTGATCCGGAATGCCATTGCTTCCATCCTGAGGGCCTGGCTTGAC CAGTGTGCGGAAGACTTCCGGGAGCCCCCTCACTTCCCTTGCCTTCAGAAGCTGCTGGAGTACCTCAAAC AGATGATGCCTGGCTCTGACCCAGAGAGGAGAGCACAGAACCTTCTTGAACAGTTTCAAAAGCAGGACGT GGATTCCGACAATGGACTTCTCAACACCAGCTCCTTCAGCCTGGAAGAGGAAGAGGAACTGGAGAGCGGA GGGTCAGCAGAATTCACGAACTTCTCAGAAGATCTCGTGGCAGAACAGCTGACCTACATGGACGCACAAC TATTCAAGAAGGTAGTGCCTCACCATTGCCTGGGCTGTATTTGGTCTCAGCGGGATAAAAAGGAAAACAA GCATTTGGCTCCTACGATCCGTGCCACCATCTCTCAGTTTAATACGCTCACCAAGTGTGTTGTCAGCACC GTCCTGGGGAGCAAGGAACTCAAAACTCAGCAGCGAGCCAGAGTCATCGAGAAGTGGATCAACATTGCTC ACGAATGTAGAATCCTGAAGAATTTTTCCTCCTTGAGGGCCATCGTTTCCGCACTGCAGTCTAATTCCAT CTATCGGTTGAAAAAGGCTTGGGCTGCTGTCCCGAAGGACAGAATGCTGATGTTTGAAGAACTTTCAGAT ATCTTCTCTGATCACAATAACCATCTAACCAGTCGGGAGCTACTAATGAAGGAAGGAACTTCAAAATTTG CAAACCTGGACAGCAGCGTGAAAGAAAACCAGAAGCGGACCCAGAGGCGCCTGCAACTGCAGAAGGATAT GGGTGTGATGCAGGGTACCGTGCCTTACCTGGGCACCTTCCTGACTGACCTGACCATGCTGGACACTGCC CTGCAGGACTACATTGAGGGTGGACTGATCAACTTCGAGAAAAGAAGAAGGGAATTTGAAGTCATTGCCC AGATAAAGCTCCTACAGTCTGCTTGCAACAGCTACTGCATGGGCCCAGACCAGAAGTTTATCCAGTGGTT CCAGAGGCAGCAGCTTCTATCAGAGGAGGAAAGCTACGCCCTCTCGTGTGAGATTGAAGCCGCTGCCGAC GCCAACACCACTTCCCCTAAGCCTCGGAAAAGCATGGTGAAGAGGCTGAGCCTGCTATTTCTGGGGTCTG ACATCATCCCCGGGAGCACTCCCACCAAAGAGCAGCCCAAGTCCGCAGCCAGTGGGAGCTCTGGGGAGAG TATGGACTCAGTCAGTGTGTCGTCCTGTGAATCAAACCACTCCGAGGCTGAGGAGGGCCCCGTCACACCC ATGGACACACCAGATGAGCCCCAAAAGAAGCTCTCTGAATCCTCCTCTTCCTGTTCCTCCATCCATTCCA TGGACACGAATTCCTCAGGGATGTCGTCCCTAATCAACCCCCTGTCCTCCCCTCCAACGTGCAACAACAA TCCTAAAATCCACAAGCGCTCCGTCTCCGTGACATCCATTACCTCCACAGTACTGCCTCCTGTTTACAAT CAGCAGAACGAAGACACCTGCATCATCCGCATCAGTGTAGAAGACAACAATGGCAACATGTACAAGAGCA TCATGCTGACAAGCCAGGATAAGACCCCCGCTGTGATCCAGAGAGCGATGTCGAAGCACAACCTGGAGTC GGACCCCGCCGAGGAGTATGAGCTGGTGCAGGTCATCTCGGAGGACAAAGAACTAGTGATCCCGGACTCT GCAAACGTCTTTTACGCCATGAATAGCCAAGTGAACTTTGATTTCATTTTACGCAAAAAGAACTCGGTGG AGGAGCAGGTGAAGTTGCGCAGTCGGACCAGCCTGACTTTGCCCAGGACAGCTAAGCGGGGCTGCTGGAG TAACAGGCACAGCAAGATCACCCTCTGAAAGGGACAGTACACTCCTACTGCCCAAGGCAGAGTGAGGCTG AGCAAAAGCCATGGCGACGCCAACCACCACCCAGTGTTGAGCATCATTGGTGAAAGCGACAGATATTTAT AGAATTCAGCTGTGCAGAGAGCACTGTGCAGGGGAGAGTGGAAGTGAATTTGACATTAAAAGGATAAAAG GTTCACTGATGCTCTTAGATCTGAGAGTGAATACAAAATAGTCCATATTTTAAAATATATATACACACGC ATATCGTGTGTGTATGCGTGTGTTTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATG TAGATATACCAGAGGGACTATACAGGATATTCTGGACTATGGAAAAACCAATTTGCACAATGGCCTGGGA AGTTGAGGTCACTTTTTACAGGGAAACAGAAGGGACCAAGAACCTGGTCTCATACTTCCAAGTAAATAGA ATTAGAACTACCAATGCGGAGTGTTCTTCATGCCAGTATTACAAGCCCAGGCTCTTATTTTTGTAAAGGG AGAGAATTCCTTTCTCAGTCATGTGCCACCAGATGACCAAACAGCAGAGATTGACATGGCCATAGGCTGC ATGAAAGGCCACCATGGAAAGGTTCGGATGGGTCAGTGGTCTCTGCCCCCTGCTGGAGTCTGCTGGAGCA TCTGAGGCCTGTGTGCCTTGCTGGTCCCCGTCACAATGCCCCTGGGGTCAACACAAGCTTGTTTCAATTC CTGGAAACCCTGTTGTGTTTCTGCATTCTCTCCTGAGGAACGCACCACAATCATTACCACACAGGTAGCC TCGGTCTTCCATAACTTTTCAGCCAGAGAAGAAAGCGCAGAAGCACAGCAACTCCTCTGGACTCTCCTCT GTCTTGGACCACTCCACCACCCTCTGATTGCCTTTGCATGTAGCAGTGTACATCCGGTCGTTTGCCATCT TTAGCTGTGGGCCTCTGTCTTATGAGCAGTTCTTCCTCAGGTGTAGACATTTCTGCTGCAGTCAACTCCA TGCAATCATTTCAAGCCTGTGAAAGATTTCTGAGCAGCAACCCCCCCCCCCCCCCCCCGGTCAACCCCTC AGAAACTGTCTCCCACTGACTAACAGCGGCTCCTGTTTCCTTCTTTACTTTAGTAGCATTCCTGTGTTCA GGTCACAGGGAGAGAAAGGAGACTGCGACTGCCGTCTCTGTCCTTTCTCACAGAGGTGCACCCTTATAGA AGGGAAGAGTCGCGCTCTTCTTTCTCACCTCTCACTTACTCTCTTGGTGGCGCTGGTAAGTGATGGGTTT TGTCTGTAATAACAGTTTCATTCTGTGCTTCCGATCTGCGCTATGCTGGGATCTGAATGGAAAAGCCATA CATGAAGGAGCTGACATGTATAAGAAGCACTTTCAAATTATTGTCCCTTTTTAATTTTTTTCTCCAAATA CCAGTTTTTATTCCAAAAATAAAGAGAATGAACCCAGAATACGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241244 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241244 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7492 same embryo
	EMAGE:7493 same embryo
	EMAGE:7490 same embryo
	EMAGE:7489 same embryo
	EMAGE:7488 same embryo
	EurExpress:euxassay_004442 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source