EMAGE mouse gene expression spatial database

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EMAGE:7493

Ceacam2 carcinoembryonic antigen-related cell adhesion molecule 2 ( MGI:1347246)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004431. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004431_01	euxassay_004431_02	euxassay_004431_03	euxassay_004431_04

euxassay_004431_05	euxassay_004431_06	euxassay_004431_07	euxassay_004431_08	euxassay_004431_09

euxassay_004431_10	euxassay_004431_11	euxassay_004431_12	euxassay_004431_13	euxassay_004431_14

euxassay_004431_15	euxassay_004431_16	euxassay_004431_17	euxassay_004431_18	euxassay_004431_19

euxassay_004431_20	euxassay_004431_21	euxassay_004431_22	euxassay_004431_23	euxassay_004431_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7493_wholemount_strong.wlz

7493_wholemount_moderate.wlz

7493_wholemount_weak.wlz

7493_wholemount_possible.wlz

7493_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7493_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7560
Entity Detected:	Ceacam2, carcinoembryonic antigen-related cell adhesion molecule 2 ( MGI:1347246)
Sequence:	sense strand is shown >T7560 GACCCACGCGTCCGCCCACGCGTCCGAGAAGCTAGCAGGCAGAAGAGACATGGAGCTGGCCTCAGCACAT CTCCACAAAGGGCAGGTTCCCTGGTTTGGACTACTGCTCACAGCCTCACTTTTAGCCTCCTGGAGCCCTC CCACCACTGCACAAGTGACTGTTATGGCTTTTCCACTCCACGCTGCTGAAGGCAACAATGTTATTCTAGT TGTTTACAATATGATGAAGGGAGTCTCGGCCTTTAGCTGGCACAAGGGATCTACTACGTCTACAAATGCT GAAATTGTACGATTTGTAACAGGCACTAATAAGACTATAAAAGGGCCTGTACACAGTGGCAGAGAGACAC TATACAGCAATGGATCCCTGCTCATCCAAAGGGTCACCATGAAGGATACGGGAGTCTACACAATAGAAAT GACAGATCAAAACTATCGTCGTAGGGTCCTGACTGGACAATTTCATGTACACAAGCCAGTGACTCAGCCC TCCCTCCAAGTCACCAACACCACAGTCAAAGAACTAGACTCTGTCACCCTGACCTGCTTGTCGAAAGACC GTCAAGCCCACATCCATTGGATCTTCAACAATGATACTCTTCTAATCACAGAGAAGATGACAACCTCTCA GGCGGGACTCATCCTCAAAATAGACCCTATTAAGAGGGAAGATGCCGGCGAGTATCAGTGTGAAATCTCG AATCCAGTCAGCGTCAAGAGGAGCAACTCAATCAAACTGGAAGTAATATTTGACTCAACATATGACATTT CAGATGTCCCCATTGCTGTAATCATAACTGGAGCTGTGGCCGGGGTGATTCTAATAGCAGGGCTGGCATA TCGCCTCTGTTCCAGGAAGTCTCGCTGATCTGGCTCCTTCTGACAACTCTCCTAACAAGGTGGATGACGT CGCATACACTGTCCTGAACTTCAATTCCCAGCAACCCAACCGGCCAACTTCAGCCCCTTCTTCTCCAAGA GCCACAGAAACAGTTTATTCAGAAGTAAAAAAGAAGTGAGCATAATCTGTCCGTCTGTCCTGCTGGCTGC ACCAGTGATGCATTCCCAGATTCTCTCCCTCACTGGAGGGTCTCTGCACACACACACACACACACACACA CACACACACACACACACACACACACACACTCACTCATGCATTTACTCTGTTGACTCCTTCAGTGTCTATA GAAGAAAAGGTGGATCCTGGAGCCTACAGAAAACTCAACCCTTCTAGGCTTTCAAATTTGGCTGAGAGTG AGGTATCAAAATTTCTCACCCTTTCACTTTCCTGACCCAGACTGTTTAAAATTGACCTATTCAGAGCACC TTCATTCCCCTCCCAACTCCAAGTCCTGCCCTGTCAGAGTCTGACTTGAATTTCCATAAACCTTGGAGGT CACCTAAGTGCTTACGCCAAACAAAACAAAACAAAACAAACCAGAAGCAGGAAATGGCCAGTCCCATCTC TTTAAAGGCTGATTGGAAGCCACCACACATGAGAAGATCAAACCTCCATGGGCAATCTACACACCCGACA ACTGTCATGCTTACCCATCTGGGGCATTCGAGTCTCTGAACCTTGTGTCCTCACGCCTGAGCCCTTCTCT GAGCCTTTCTCCAGAAAATCCACTCACAGCAACTAGAGAGGCTCTTTGTCAGCAACTCCAAGCAAACTGC TAGGCAGGATTCAGAAGAAAAGACAGCATCTCTAACATCCACCAGGAAGGTGCCCAGAAAAGCAGAGCTG GTGACTTTGGACTGACAGACATCTGGAGTGTGAAAAAGCAGCACAGAGCTAACCTTCGGAGAGTGTTGAA ATTATTTGAAAAGAAGCCATATTTGGAGGTATTGGAGTTTTCCTCTTTCTGAGACAATCCACTATTTGAA AATTGTAGCTACTGAATTGCCTCTCAGTATGCAAGCTGATCACTTTGCCTTAGGGCCACTAGATTTCTGT CTCCCTTGGCCCCTCAAGCCCTTTTGATCATGAGTCCCAAACCAAAAATAAATGAACACTGAGGCAGTCC CTTGCAGAACCACTGTCATGGGTCAGGCTAAGCCTCCTGCTTTTCTGAATTAGTCAAGAAAAGCCTTGGT TTCCCTTTTTCCATCTTTTTATCTTGTCTTTCAGATACTGGCCAGAGCCTGGACACTCTTCCTCTGAGAT CTCCAGCTTCTCTGCCTTCTTGTGTTTCTTTTAAACTCTAACAAAAACTGTTCTCACCTTCAAAAAATAA AATAATAACAAGCTTTCCACATCCCCACCAAAGAGGGACCCAGCTAGGTTTCTGGGAACCCAGCACCAGC CTCCAGCTGCCCTTCTGCAGTGTTTCTGCCTCTGTTTCCCTTTCGTTTTGACTTTTTTCCTTCTTTTGAG ACAGAGTTCCAGCATGGAGCCTGTGCAGGTTTCAATCCCACAGTAACACCTTCTGCAGCACCCCACCTGC TCAGACTGCAGCCCTGGCCACCAGGCCTGGCTACCTGGACATTCTGTCTGTCCTGCACTCTCAGGAAACC TTGGCCTCTGCTACTGTCTGTTTGGCTCATTCAAAGTGTGTCCTTAAAGGAATGCAGTCACCCATGCCAG AGGCAGTGTTTACAGCCTGGAATGCTCTGCACTTCCAGTGGACCAGTGCTCCACCGGAAGTGGGCTGTTA GCAGGGTCCTCTCACCTGGCCCTGGCCTTTCTGTAGCCTTGAATCCTGCCTTCCCCACCAGGGCACCAGG GATGAGTGCAGCAGCAGGAGGAGAGGCAAACAGTCACCTCAGGAACCTTCTGAGCTAAGGCACACCCTCT GTGCCTGTCAAGCAAAGGTTGTATTGGATATCAAGTGTTTGGTCTCACGCCAAGCCAACAGGCTTTGGAG AGAATTAATTAGTTCTCCTACTCAGGGATTTCTTTCAGTCCTAACACAGCCTGTGTATATTTTGCTTCAC CCACGCAATGCTGGATTATTTAATTTTGCCCGGCTTAAGACAAATCTGAGTTACTTGTAAATTTGCTCTA TGTTCATAAAAAAAGTTCATTATATATCACTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4241449 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4241449 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7492 same embryo
	EMAGE:7491 same embryo
	EMAGE:7490 same embryo
	EMAGE:7489 same embryo
	EMAGE:7488 same embryo
	EurExpress:euxassay_004431 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source