EMAGE mouse gene expression spatial database

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EMAGE:7494

Suclg2 succinate-Coenzyme A ligase, GDP-forming, beta subunit ( MGI:1306824)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005230. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005230_01	euxassay_005230_02	euxassay_005230_03	euxassay_005230_04

euxassay_005230_05	euxassay_005230_06	euxassay_005230_07	euxassay_005230_08	euxassay_005230_09

euxassay_005230_10	euxassay_005230_11	euxassay_005230_12	euxassay_005230_13	euxassay_005230_14

euxassay_005230_15	euxassay_005230_16	euxassay_005230_17	euxassay_005230_18	euxassay_005230_19

euxassay_005230_20	euxassay_005230_21	euxassay_005230_22	euxassay_005230_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7494_wholemount_strong.wlz

7494_wholemount_moderate.wlz

7494_wholemount_weak.wlz

7494_wholemount_possible.wlz

7494_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7494_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7642
Entity Detected:	Suclg2, succinate-Coenzyme A ligase, GDP-forming, beta subunit ( MGI:1306824)
Sequence:	sense strand is shown >T7642 AAGAGGTAATCCGGTTCCTGGTTAAGATGGCGTCCCCGGTGGCCATCGCGGCGCAGGCTGGGAAGCTTCT GCGAGAGCGAGCGCTGCGGCCGCTCCTGGCGGTCAGGTCCCAGGCCGGTCACTTAACCCCCCGAAGATGG CTGAACCTGCAGGAGTACCAGAGCAAGAAGCTCATGTCAGAGCACGGAGTGAGAGTGCAGAGGTTCTTTG TTGCCAACACTGCTAAAGAGGCTCTGGAGGCAGCCAAGAGATTGAATGCAAAAGAAATTGTTTTAAAAGC CCAGATCTTAGCTGGAGGAAGAGGAAAAGGTGTCTTCAATAGCGGTTTGAAAGGAGGTGTTCATTTAACA AAAGACCCTAAAGTAGTGGGAGAGTTGGCTCAACAGATGATTGGTTATAATCTAGCAACGAAACAAACTC CAAAAGAAGGTGTGAAAGTTAACAAGGTGATGGTTGCTGAAGCCCTGGATATTTCTAGAGAAACATACTT GGCCATTCTAATGGACCGGTCCCATAATGGCCCTGTGATAGTGGGCAGTCCTCAGGGGGGCGTTGATATT GAGGAAGTGGCAGCTTCCAGTCCAGAACTCATCTTTAAGGAGCAGATTGACATCTTTGAAGGGATAAAGG ATAGCCAGGCCCAGCGCATGGCAGAAAATCCGGGCTTCCTTGGGTCTCTGAAAAACCAGGCTGCAGATCA GATTACAAAGCTGTACCATCTCTTCCTGAAGATTGATGCCACTCAGGTGGAAGTGAACCCCTTCGGTGAA ACTCCAGAAGGACAAGTTGTCTGTTTTGATGCCAAGATAAACTTTGATGACAATGCTGAGTTCCGACAAA AGGACATATTTGCTATGGACGACAAATCCGAGAATGAGCCCATTGAAAATGAAGCTGCCAGATACGATCT CAAGTATATAGGACTGGACGGGAACATTGCCTGCTTCGTGAATGGTGCTGGGCTGGCCATGGCTACCTGC GACATCATCTTCCTGAACGGAGGGAAGCCAGCGAACTTCTTGGACCTTGGAGGTGGTGTAAAGGAAGCCC AAGTCTATGAAGCATTCAAACTGCTCACGTCTGACCCCAAGGTGGAAGCCATTCTCGTCAATATCTTTGG TGGGATCGTCAACTGTGCCATCATTGCCAACGGAATCACCAAAGCCTGCCGGGAGCTGGAGCTCAAGGTG CCACTGGTAGTCCGGCTTGAAGGAACTAATGTGCAGGAGGCTCAGAATATCCTCAAGAGCAGCGGACTCC CCATCACGTCAGCTGTGGACCTGGAGGATGCAGCCAAGAAAGCTGTTGCCAGTGTGGCAAAGAAGTGATG CTCTCCCTGGCTCCCATGAAGAAGGGCATGTCCCTGACTACTGTGAAAGCAATTGTTTTCTCACTGGGAA GGGACACACACAAGGATCATCATGTGAAAAATATTAACTCTGAACTTTCTCAAATACGTTATCCAGAGTG CCTAAAGCTGAATTTATACTGTAGTCATTGTTAAAAAAAAAAATCACTCCTTACAATTTCACATCTTCTC TTGCCCATTCAGTAGCAGGGTCTACCAGCTCTGGGCCACAGTCAGTTTAGCCAAATAGTGTGCATACATA CAGTATTTAAAGCCAGATCTAGGTTCATTCACAAGGGCATTTATTCTACATTAGAAAATTTTACAGGACC CTCAAGTTCAGACAGGCTGATATTTAACATAGTGGCTTCCAGAAGGGTACAGCTAAGACACCTGAGCTTC TGTGACCAGTGGCCTCCACTGGCCCCTAATTCCAGTACATTAAGAAAGCCCATGAACATTTCTAAAGTGT GGCTTATATTGGGCATCCCGTGTTGATATATGTACATTGCACACATTTTACCCTGTGCCTTAATGTTGAA TCTACTGTTTGCCTCACACTAAGTTACTACCAGAAGCCTTAGTCATGGGTGTGAGGTGGAAGGTTTCATT TTGTGTCAGAGTTGCTGAATGTTAATCTATTTCATAAAGAGTCACACTAATGATCTAGCTGCTGGTAGCG GCCAGGCTTTCCCCTCTTGTCCCCTCCACCTCACATCTATCAGTTTGCCACAGAAAGATGAGAGATGCCA TTGGACAGAATGTGACCTTCCTAATGATGTCCTTTTTTGTGACAGGAATTTTCTTAACTTGATTTTTTTG ATTTAATTTTGATAAATTTATAGTCCTATACATTTTAAATAAAATTGTTTTTCAGTGCTAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:30470296 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30470296 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7497 same embryo
	EMAGE:7495 same embryo
	EMAGE:7498 same embryo
	EMAGE:7499 same embryo
	EMAGE:7496 same embryo
	EurExpress:euxassay_005230 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source