EMAGE mouse gene expression spatial database

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EMAGE:7497

9930012K11Rik RIKEN cDNA 9930012K11 gene ( MGI:2145726)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005203. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005203_01	euxassay_005203_02	euxassay_005203_03	euxassay_005203_04

euxassay_005203_05	euxassay_005203_06	euxassay_005203_07	euxassay_005203_08	euxassay_005203_09

euxassay_005203_10	euxassay_005203_11	euxassay_005203_12	euxassay_005203_13	euxassay_005203_14

euxassay_005203_15	euxassay_005203_16	euxassay_005203_17	euxassay_005203_18	euxassay_005203_19

euxassay_005203_20	euxassay_005203_21	euxassay_005203_22	euxassay_005203_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7497_wholemount_strong.wlz

7497_wholemount_moderate.wlz

7497_wholemount_weak.wlz

7497_wholemount_possible.wlz

7497_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7497_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7643
Entity Detected:	9930012K11Rik, RIKEN cDNA 9930012K11 gene ( MGI:2145726)
Sequence:	sense strand is shown >T7643 AAGAGGTAATCCGGGCAGAGCCGCTCCAGCACAGCTAGGGACTTGGGGACGCCCGAGATCACTCCGGTGC CGCTGCAGCGGTTTCCACGTCTGGAACACGCCATGCTGAGCCGGCGCCGCGTCTTTGCGGTGGAGCGACT TGGCGGCCGGGATGGGGCCTTTGAGGACCTGGCACAAGGCTGTGTGGTGCCAGGAGTCACTTGCACCTAC AGACGGATACCAGACAACACTCACGAATGTTCACTGGACTTCAGGGAGGGACAGAATGAGCTGAGAGGTC TGGAGAGACAGATGCCACTTCTGAAACTGGCTTCCCAGGACTCGGGCATGGAGATGGTTGTTGGGGACAG CTCCTTGGCTACCTTATCAGGACGTTCTCAAGATTCTCTGAACCTTGAGCCCATGGGGAGCCCTGAGCTG CCTCCTGCTCAGCTAGACCGGCTGCTGGCCAGACAGAAGCTGGAGGAGGTGCTGGAGAGATCTCGAGAGT TTCCCAGCTTGTCTGCGCAGCGTGGCCCCCTGCAACTGCTGAACAAGCCTGTAGATGGAGTGCCCATCTT TGCAGGAGAACAGGAGTCCACGGAGGCAGACACTGAGCTAGAGGCTGGTCTGGAAGAAGCAAAGGAGGTT GGTAATATGGAGTCTGCAGCCTGTACCTGCCTTCCAGGTCAGGGCTTGCGCTATCTGGAACACCTGTGCC TTGTGCTGGAGCAGATGGTGAGGCTCCAGCAGCTGTACCTGCAGCTGCAGACCCAGAGACCCTCTAGGGA TCCTGAGGAGGAAGTATTGGCTCCAGCTCTTTCATCATCACATATCCCAGACAATAGGGTCCAGGAGCAC AGAGAGGAGCTAAGCCAGACAAAGGACCCAGAAGGTGCAGAGGCAGCTTCACTTCCAGAAGTGGGGGTGC TTGTCACCAGCCCCTCTAGGCTGCCAGAGGCCTTGCTGGAACCAACCCACGTCCATCCACCCTCCCAGGA ACCCAAGCAGGATCTCTCCCACTGGGATAAGGTCAAAGTTCTGCTCAATCGGCTCCGTTGGAGGAGCCCT CGACTTCCTGAGCCTCCTGTTCCCCCTGATGGTTCTGGATCCAGGATGGAGTTTAGAAACCTCTCTGACA GGACTCCGTGCCATTCCCAACGGAAGACCTTTATACCAGCATTGGTGGTTAAGAAGCCACGAGCAAAAAA TCTTTCTGTATGACCTCTTGGTGTACCTGGTGACCTTGGGAAGAAGTCCCTCACATTTTGGCTTACTGCT GCTTCTCTGCACTGCCAAGAAAAGCTATTGGTGCTTGCTCCTCTTCAAGGTGAAGGCCAAATCCATCTCT AAGCATCGAAGCAAGGAGCCCTGGATCCCCAAGAGGTTCCTGAGTGCAGTTCCAGCACTCTTCCAGGGCT CTTCCTCCTCATCAGAAATCTCTGGGCTTAGGCAATGTGAATTGACTTATTTATCAGGTGCTCATAGAGC ATGTTTAGTATGGGAGTGTGTCAGCATGTTGCAGATGTAAGTTTATGCAATCTTTGTTGGACTTCCGTCT GCCCCATCTGATGGAATCATTCGTCCAAAGGGTTGGTGTTTGGGGTGTCACTGGAAGGATGGCACCGATC TTCATTCCACAATGAACAGTTACTAATGTGGACTTCTAATGTCTTCCTCATACCTGAGACGGCAGCCCTA GGACCAAGAACAACATTGCCTTGGGTACCTTTGTAGTGAGTCACGGCTCTGGAGTACAGCCCCTGAAGGC TGAATGAGGGATGGGGGGAAGGTCAAGAACATTGAGAGGAACAACACATAACTTGCAAGGTCAACTCTAA GATGCTGTGTTCTATCCTGGAGTTTAACCATAGCGAAGTCTCTTAATTTCTGAGCCACAGCTTCCTACTT GGGACCATGATACCCACCTTACCTACCTCATAAGCTTGTTAGGAGGATGAAGTAACATTAAACATTCTCA ATGTGTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30433488 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30433488 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7495 same embryo
	EMAGE:7498 same embryo
	EMAGE:7499 same embryo
	EMAGE:7496 same embryo
	EMAGE:7494 same embryo
	EurExpress:euxassay_005203 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source