EMAGE mouse gene expression spatial database

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EMAGE:7520

Tbrg3 transforming growth factor beta regulated gene 3 ( MGI:1100876)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004630. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004630_01	euxassay_004630_02	euxassay_004630_03	euxassay_004630_04

euxassay_004630_05	euxassay_004630_06	euxassay_004630_07	euxassay_004630_08	euxassay_004630_09

euxassay_004630_10	euxassay_004630_11	euxassay_004630_12	euxassay_004630_13	euxassay_004630_14

euxassay_004630_15	euxassay_004630_16	euxassay_004630_17	euxassay_004630_18	euxassay_004630_19

euxassay_004630_20	euxassay_004630_21	euxassay_004630_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7520_wholemount_strong.wlz

7520_wholemount_moderate.wlz

7520_wholemount_weak.wlz

7520_wholemount_possible.wlz

7520_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7520_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7675
Entity Detected:	Tbrg3, transforming growth factor beta regulated gene 3 ( MGI:1100876)
Sequence:	sense strand is shown >T7675 AAGAGGTAATCCCAATTACACTTCTGTTAAAGACTCCCTTGCAGGCCAGACATTTCGAAGGTTTATTTGA GTTTGGTTTGGTGGTTCTTCCACTCCGGTTTTGTGCTCGCTCTCATTTTGATCATAGGAACTTTACTGAG AGATGTAAGATGCGCCTCACTGGCTTGAGAAGTAACTTGGTCAGGGTAGTTTGTGCTCAATTCCCAGTGC TTTTCTGTGCTTCTGAAGAGAAAACGCACACTATTAATAGCAGTCTAGCTTATGTTGAATGCACATTATT ATGCTTTTTTTTTTTCCTTTGTGATAAGAGTTTTCCGAAAAAGCACTGGCTAATTGGGAAATATGGATGC GATGATTGGGTCTGTTTTTGTTGGAAGCCCTTTCCACGTGGGGAAGGGATGCTATTTCAAGATCTTTTAG GTGACTCTTACTTGGCTAACTTCAAATTCCAATTTCAGTCTTGGAGTAGGGATAAAGTAGAATTAGCTAT GCGATGTAGTAGGCTTGCAGAATTAATATCCACCTCAGGATGCAGTAAATGGTTTGCATCCCCGGTTTAT TCCTAGTGGTAGCAGGGTACTTCCCTGGCAGATGCTGAAGAACCCGTTAGTTTTACTGATTTTCTGGTGT GTGAGGGCCCTGGGCCATATGCTTTTGAATGGTAAGTACTCTTAGACCAACTTGGATTTTGCTCTTGTTT GTGGAAGACTTTGTTTTGTTTTGTTTTTGTTTGTTTTTTTGTTTTTGCTCAATTCCCCATAAAATATAGC TCACTATGGTTTTTTTAGATCATTTTTTAAAAAATTTTGGTCTGTATAGCCTTAATGGTCTTTAAACGTG GATGGCAAAATGGGCTCATAATGTTTATCTTGTGTATGTGTTAAAAGAAGGTTTTCTTTTATATTAGTTA AAATCATGCCTGTCACATAAGGAGACACTTAAGATTATACATATGAGTTATTTGGAATTTTTTATTAGGA CTTGAATTATGACCTCATGTTTACTGTATGATTTGAAAAAGTGCCAGTTACTTGAATTAGAAGCACCTCT CACACTCAAGTATGTATTATGCTTGTTTTATGGCTTATGAAAAAATTACTATAAAGCTTTACTGTGGTAG TTTGTGAGTGTGTATGTATGTACGTATGTATATGAGAGATCTTACATAGGTAAGATAGTAGGAGAATTGC CTTTATTAAGTTTTAACTTAGAGAGGAAAGGAAGCTAACATTTACGTTAAAAACAAAAACAATGTATGGA CTTAGGCCAAAGAATTCTAAACTGTCCTAAAATGTCTACCCAGTGGACCTAAGAGTTCATTATTCCTTCT CCACAGAATACATAAGCTAGTGAGAGGTGGAGCTTGAATATAACTCTTCTCTCCTAGGTCCCAAAACTTG TTACCAGGTTCCTTTTAACTCAGGGCCTGCTCAAGGGGCAGATTAGTTAGTATGGGTGGCCCTAAAATAC TTAGCTTTCTACTGTCATTCTGCTCTACTGGCTCTTTAAAACAAACAAACAAACAAACAAACAAACAACA GCACAACAAGCAATAACAACCAAACTGGTGAATATGAAATTTGGAGCTGGGTGAAATACTGGAAGGTGGT TTCTGTGACCCTTGGGAATTTTCTTAACTTTCAGTTTGTCCTTGCTTGGCCAGTTTTGCTGGTATGCCTA AGATTTAATTTGTAAGACGTTTCTCTTTCTTATCTACTTCTGCCATTTTGTCTTGAGCTGAAAACATAAC CTGAAAGAGCAAGAGAAGTCATAAAAGGTTGTCTTTCTTGTTGCCTGTGTGACTGGTTTGCAAGTGTTCA GGAACCATGTCATGATTGCTTTCACTTTGGCCAAGTGAGACTTTTTTGACATAAATTTATGAAAAACAGA ACTTGTCCCAGGATGCCTTAAGCATTACTCCCTCTTGGGGGAGTATTTTTAGTTTTAGGCCCTTGAAAGT CTTGTGGAGGTGATACTGGGGATTGAGAGCAGTCTCAATTGTGCTAGACAGGCAGGTATTCTGCTAGTGA ACTATATATACACCCAAGCTTCCCAAGGAGCTTGTCATGACCATGGTACCCACAGTACAGAGGAAAAGAG TCTCATGGTGACAAAATTCAGAGTTTCCTATATTAGGGCCGTGCTTGCTTTGAGGTAATGCACAGGACTA GCAGGAAGATATGCTATGTAACAGGAGTTGTTGGTATAGCTGTGGCTTTATTTGGTGGTTCTTTATGGAT TTAAGAACATAGAGCTGGCTGTGGTGGTATATGCTTTTAGTCCCAGTACTCTCAAAGGCAGGCAGGTCTC TATGACTTTGAGGCTAGCCTGGTCTACATAGTGTTCCAGTCTCAAAAACAAACAAACAAACAAACAAAAA AAACAAAAGAAACTCTCTCTGTAGGATTGGGACACTTGACTATTAACACAATATGGAGATGGTTTCTTTG TTTGTACATTGTATCCCTAAATGTTGATTAAAATCCTCTTGTATATGTCAGGCCATTTGCATTAATAGAT GTTCATGGGCTACATTAAAAGGTAGAAGAAGTGGAAAGCAGACTATAAACAGTAAGTAAATAAGAATGTC ATATAGTAATAGCAACAAAGAGAATAAAAAAATATAGGTGGATGTGTTGGTTCCTGCTTATAATCTGGGA ACTCATGAGACAGAAGCAGGATTGTGAGTTTAAAGCCAGCTTGAGCTACATGAGCAAGTCTATCTGGAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30112263 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30112263 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7521 same embryo
	EMAGE:7517 same embryo
	EMAGE:7519 same embryo
	EMAGE:7522 same embryo
	EMAGE:7518 same embryo
	EurExpress:euxassay_004630 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source