EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7523

Nop58 NOP58 ribonucleoprotein homolog (yeast) ( MGI:1933184)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004676. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004676_01	euxassay_004676_02	euxassay_004676_03	euxassay_004676_04

euxassay_004676_05	euxassay_004676_06	euxassay_004676_07	euxassay_004676_08	euxassay_004676_09

euxassay_004676_10	euxassay_004676_11	euxassay_004676_12	euxassay_004676_13	euxassay_004676_14

euxassay_004676_15	euxassay_004676_16	euxassay_004676_17	euxassay_004676_18	euxassay_004676_19

euxassay_004676_20	euxassay_004676_21	euxassay_004676_22	euxassay_004676_23	euxassay_004676_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7523_wholemount_strong.wlz

7523_wholemount_moderate.wlz

7523_wholemount_weak.wlz

7523_wholemount_possible.wlz

7523_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7523_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8625
Entity Detected:	Nop58, NOP58 ribonucleoprotein homolog (yeast) ( MGI:1933184)
Sequence:	sense strand is shown >T8625 GGTCTGTTCGTCCGCGGGGAGGCGACGGCGTTTGCGCTCCCCCGCGTCCAAGGCCTAGCGGGTCTTCAGC GACTGAAAGGTAGTCATTTGCAGTGGCGTAAGGGCTCGCGGCTAGCCGCGTAGCGCCGCCCTGACCTGGT CTCATCATGTTGGTCCTGTTTGAAACGTCCGTTGGCTACGCCATCTTTAAGGTTCTGAATGAGAAGAAAC TTCAAGAGGTTGATAGTTTGTGGAAAGAATTTGAAACTCCAGAGAAAGCAAATAAAATAGTAAAGCTAAA ACATTTTGAGAAATTTCAGGATACAGCAGAAGCATTAGCAGCATTCACAGCTCTGATGGAAGGCAAAATC AATAAACAGCTGAAGAAAGTTTTGAAGAAAATAGTAAAAGAAGCCCATGAACCTCTGGCTGTCGCTGATG CTAAACTAGGAGGGGTAATAAAGGAAAAATTGAATCTCAGCTGTATCCATAGTCCTGTTGTTAATGAACT TATGAGAGGAATCCGATCACAAATGGATGGCTTGATTCCTGGGGTAGAACCACGTGAGATGGCAGCCATG TGTCTTGGATTGGCCCACAGCCTGTCTCGATACAGATTGAAATTCAGTGCTGATAAAGTGGACACAATGA TTGTTCAGGCAATTTCCTTATTAGATGACTTGGATAAAGAACTAAACAACTACATTATGCGGTGTAGGGA ATGGTATGGCTGGCATTTCCCTGAGTTAGGGAAAATTATTTCAGATAATCTGACATACTGCAAGTGTTTA CAGAAAGTTGGGGACAGGAAGAACTATGCATCTGCCTCTCTCTCTGAATTCCTGTCTGAGGAAGTAGAAG CAGAAGTGAAAGCAGCTGCAGAGATATCTATGGGAACAGAGGTTTCTGAAGAAGATATTTGCAACATTCT ACATCTGTGTACGCAGGTAATTGAAATTTCTGAATATAGGACTCAGCTCTATGAATATCTGCAAAATCGA ATGATGGCCATTGCACCTAACGTTACAGTCATGGTTGGAGAATTAGTTGGAGCACGGCTTATTGCTCATG CAGGTTCTCTTTTGAATTTGGCCAAGCATGCAGCTTCTACAGTTCAGATTCTTGGAGCAGAAAAAGCACT TTTCAGGGCCCTCAAATCTAGACGAGACACACCTAAATATGGACTTATTTATCATGCTTCTCTTGTAGGC CAGTCAAGTCCCAAACACAAAGGAAAGATTTCTCGAATGCTGGCAGCCAAAACTGTTCTGGCTATCAGAT ACGACGCTTTTGGTGAAGATTCCAGTTCTGCAATGGGAATTGAGAACAGAGCCAAATTAGAGGCCAGGTT GAGGATTTTGGAGGACAGAGGGATAAGAAAAATAAGTGGAACAGGAAAGGCATTAGCAAAAGCAGAAAAG TATGAACACAAAAGTGAAGTGAAGACTTACGATCCCTCTGGTGACTCCACACTTCCAACTTGTTCTAAAA AACGCAAAATAGAAGAGGTAGATAAAGAGGATGAAATTACTGAAAAGAAAGCAAAAAAAGCCAAGATTAA AATTAAAGCTGAAGTAGAGGAGGAGATGGAGGAGGAGGAGGCGGAGGAAGAACAGGTAGTAGAAGAGGAG CCGACTGTAAAGAAGAAAAAGAAGAAGGATAAAAAGAAACACATAAAGGAAGAGCCACTTTCCGAGGAGG AGCCATGTACCAGCACAGCAGTTCCTAGTCCAGAGAAAAAGAAGAAAAAGAAAAAGAAGAAAGATGCTGA AGACTAATGTAAAGGAACCATAGCCCTGTCCCCGAACACATCATGCTTACGATTCAGTTGGGAGCAGAGC ACATCAGATGCTCTCAAATAATCAAGGGAGGTTGAATATCTAGCTTTTCAAGCCTTTTTGTGTCTTGACA TCAACTGTTGACCTTAGTCTTTGATACACAAATAAAATATTTTCTTTGTATTATAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:5718488 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5718488 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7527 same embryo
	EMAGE:7526 same embryo
	EMAGE:7525 same embryo
	EMAGE:7524 same embryo
	EMAGE:7528 same embryo
	EurExpress:euxassay_004676 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source