EMAGE mouse gene expression spatial database

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EMAGE:7527

Aplp2 amyloid beta (A4) precursor-like protein 2 ( MGI:88047)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004667. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004667_01	euxassay_004667_02	euxassay_004667_03	euxassay_004667_04

euxassay_004667_05	euxassay_004667_06	euxassay_004667_07	euxassay_004667_08	euxassay_004667_09

euxassay_004667_10	euxassay_004667_11	euxassay_004667_12	euxassay_004667_13	euxassay_004667_14

euxassay_004667_15	euxassay_004667_16	euxassay_004667_17	euxassay_004667_18	euxassay_004667_19

euxassay_004667_20	euxassay_004667_21	euxassay_004667_22	euxassay_004667_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7527_wholemount_strong.wlz

7527_wholemount_moderate.wlz

7527_wholemount_weak.wlz

7527_wholemount_possible.wlz

7527_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7527_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 03 04 05 18 19 20 weak expression: see section 21

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 14 15 16 17

vibrissa

moderate

regional

moderate expression: see section 02 03 04 18 weak expression: see section 05 17 19

ventral grey horn

strong

regional

strong expression: see section 08 09 10 11 12 13

bladder

moderate

regional

Expression in the fundus region.

urethra of male

moderate

regional

moderate expression: see section 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8633
Entity Detected:	Aplp2, amyloid beta (A4) precursor-like protein 2 ( MGI:88047)
Sequence:	sense strand is shown >T8633 GCTGTAGCCTGGCTGTGCGAGAGACGGGGAGAGCCCAGCGCCACAGCGACCTGGAGAAGGATGGCGGCCA CCGGGACCGCGGCCGCTGCGGCCACCGGCAAGCTTCTTGTCCTGCTGCTGCTCGGGCTCACGGCGCCCGC TGCGGCTCTGGCTGGCTACATAGAGGCTCTTGCAGCCAATGCTGGAACAGGCTTCGCTGTTGCTGAGCCT CAGATTGCGATGTTCTGTGGGAAGCTGAATATGCATGTGAACATTCAGACTGGCAAATGGGAGCCTGACC CAACAGGCACCAAGAGCTGCCTTGGAACAAAGGAGGAGGTTCTTCAGTACTGCCAGGAGATATATCCAGA GCTGCAGATCACAAATGTGATGGAAGCAAACCAGCCAGTCAATATTGATAGTTGGTGCCGAAGGGACAAA AGGCAGTGCAAGAGTCACATTGTTATACCATTCAAGTGTCTTGTGGGTGAATTTGTAAGTGATGTCCTGC TAGTTCCAGATAACTGCCAGTTTTTCCACCAAGAGCGGATGGAGGTGTGTGAGAAGCACCAGCGCTGGCA CACGTTAGTCAAGGAGGCATGTCTGACTGAGGGGCTGACCTTATATAGCTATGGCATGCTGCTGCCCTGC GGGGTAGACCAGTTCCATGGCACCGAGTATGTGTGCTGCCCTCAGACAAAGACTGTTGACTCGGACTCGA CTATGTCCAAAGAAGAGGAGGAAGAGGAAGAGGATGAAGAGGACGAAGAGGAAGACTATGATCTTGATAA AAGTGAATTTCCTACTGAAGCAGATTTGGAAGACTTCACAGAAGCAGCAGCAGATGAGGAAGAAGAGGAT GAGGAGGAAGGGGAGGAAGTGGTGGAAGACCGTGACTACTACTATGACCCCTTTAAAGGAGACGATTACA ATGAGGAGAATCCAACCGAACCCAGCAGCGAGGGCACCATTTCAGACAAGGAGATTGTTCACGATGTTAA AGTTCCCCCGACTCCCCTGCCAACCAATGATGTTGATGTGTATTTTGAGACCTCAGCGGATGATAATGAG CACGCCCGCTTCCAGAAGGCTAAGGAACAGCTGGAAATTCGACATCGAAACCGAATGGACAGGGTAAAGA AGGAATGGGAAGAGGCAGAACTTCAAGCCAAGAACCTCCCCAAGACAGAAAGACAGACCCTCATCCAGCA TTTCCAAGCTATGGTTAAAGCTTTAGAGAAAGAAGCAGCCAGTGAGAAGCAGCAGCTTGTGGAAACCCAT CTGGCCCGAGTAGAAGCCATGCTGAATGACCGCCGACGCATAGCTCTGGAAAATTACCTGGCTGCCCTGC AGTCTGACCCACCTCGGCCACATCGCATTCTTCAAGCTCTTCGTCGTTACGTCCGTGCTGAGAACAAAGA TCGCCTGCATACCATTCGTCATTACCAACACGTGTTGGCTGTTGACCCAGAAAAGGCCGCCCAGATGAAA TCCCAGGTGATGACACACCTCCATGTGATTGAAGAAAGAAGGAACCAAAGTCTTTCTCTTCTGTACAAAG TTCCTTATGTTGCTCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAACAGCGAGCGGATATGGA CCAATTTACCTCCTCCATCTCAGAGAACCCTGTGGATGTCCGGGTGAGCTCTGAGGAGAGTGAGGAGATC CCGCCGTTCCACCCTCTCCATCCCTTCCTATCCTTGTCTGAGAATGAAGGCTCTGGAATGGCAGAACAAG ACGGGGGACTGATTGGTGCAGAAGAAAAAGTGATTAACAGCAAGAATAAAATGGATGAAAATATGGTCAT TGACGAGACTCTGGATGTTAAGGAAATGATTTTCAATGCTGAGAGAGTTGGAGGCCTTGAGGAAGAGCCG GAATCGGTGGGACCTTTAAGGGAGGATTTCAGTTTGAGCAGCAATGCCCTTATTGGCTTGCTGGTTATCG CAGTGGCCATTGCTACGGTCATCGTTATCAGCCTGGTGATGCTGAGGAAGAGGCAGTACGGCACCATCAG CCACGGGATTGTGGAGGTTGACCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCAT GGTTATGAAAACCCAACCTACAAATACCTGGAGCAGATGCAGATTTAAGGACAGCGTGTGTGACACCCTG GCTGAGGCTGCTGCAGGTGGGCTGGAAGAGGCTCAGCGTTTGTGCTTGACTGCTGACCACCAGTGGTGCC AGAAGGCCTCATCCTACATCCTGCTCTCCTGGATTGTTAAGACTATAAAGTACTACTGTAGGATTGCAAT TTCCATTCTTTTAAATGGGTTAAAAAGATGTTAATATAACAATATATGATATATAAACCTTAAGTGAAAT GAAAGAAAAAGATCTATTGCAGATATCTGATGGATGTAGTTTTCTTTTTTTAAATTAGAAATGCCACTTC TATTGTATTGTCTCACACATGCTCTATATAAATGGGAAATGTTGATTTTTTTGATGATAGACTATATACA CAGGCTGTTCCCGTTATGTAAGTCAGTTCTTCAGGCTCATTTGCTGTGCTGGTTTTACTATTATTTGTTT TCAATGTATAAAGGCAGTATTTCCCTTTTCAGGTTGCTGAGAAATGTAAGAGGAGCTGAAGTACATTGTA TGCAGTTACTGACTGCTTTAGGCATAGTCTCCGCAGAAGCCTCGTGTGTCCAGTGCTGGGTGTTTGGGTG CTTCTGTACCAGCCGTCCCCAAGCAGGGCTAAGGCACCTTGAACTAGCCAGATGCAAACATAGATTCACT GTGCTTTCCTTTCAAATCCAGTTCTTCCACAGCAACTAGCCCATAGTCAGTCTGTGTTCTTCCACAGTTG TTTACGGTAGCCTTTTCCTATCCACTTCCCTTACCAAGTGTATCCAAGCGTGCACCCACCTCCTCATTGG ATGGCTCCCTCCATGCACTGACCCTCTGCCTGCCTTTGTACCTCACTCACCTCCACCGCTCTCCAGCCCC TCTGATGGCCACTCAGAGAATACCATAATAAACATGGCTGAGGACCCAGGAGAGTTCACGGGAGGCTTCA TCATATGAAAGGACAATGTTCTGGGTTGTGCATGAAATTGGATCTGTAGACACTGTGGAAACGTTTGAAA GTGTTTGTTTCCTTCATATGAACCTGTAAAATGCTGTGTAGCTGGAGTTCACGCTCACAGCCTTTAGCGC TTTGTTGCTTGTTTTGTTTTAATCTGAAGGTTCTGGTAACCTGTTTTTGTTTGTTTGTTTATTTGTTTTG GTTTGGTTTTTGTACTTTTCTGTGACTCACCTGCCCTTCCTTCCCCCCTTGGCCCTAGCCGTGCCTCGTC CCACTATGCACAGATTGAAACTTCACCTACAAACCTGTTAGTGTGATCTGTGGAGAATGTACAGAACTTA ACTACATCAATAAACACTTTAACGTCCCCTGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5719918 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5719918 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7526 same embryo
	EMAGE:7525 same embryo
	EMAGE:7524 same embryo
	EMAGE:7523 same embryo
	EMAGE:7528 same embryo
	EurExpress:euxassay_004667 same experiment
	MGI:4823180 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source