EMAGE mouse gene expression spatial database

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EMAGE:7542

1500003O03Rik RIKEN cDNA 1500003O03 gene ( MGI:1927185)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004666. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004666_01	euxassay_004666_02	euxassay_004666_03	euxassay_004666_04

euxassay_004666_05	euxassay_004666_06	euxassay_004666_07	euxassay_004666_08	euxassay_004666_09

euxassay_004666_10	euxassay_004666_11	euxassay_004666_12	euxassay_004666_13	euxassay_004666_14

euxassay_004666_15	euxassay_004666_16	euxassay_004666_17	euxassay_004666_18	euxassay_004666_19

euxassay_004666_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7542_wholemount_strong.wlz

7542_wholemount_moderate.wlz

7542_wholemount_weak.wlz

7542_wholemount_possible.wlz

7542_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7542_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8638
Entity Detected:	1500003O03Rik, RIKEN cDNA 1500003O03 gene ( MGI:1927185)
Sequence:	sense strand is shown >T8638 TCTGCCGCTGCTGCTTCTGGCGCTGCCGCTCCCGGAGGAGCTCCCGTCACGGTGATGGGGTCTCGGGCCT CCACGTTATTGCGGGACGAAGAGCTCGAGGAGATCAAGAAGGAGACTGGCTTTTCCCACAGTCAGATCAC GCGCCTGTACAGCCGCTTCACCAGCCTGGACAAAGGGGAGAACGGGACTCTCAGCCGGGAAGATTTCCAG AGGATTCCAGAACTTGCCATCAACCCACTGGGGGACCGGATCATCAATGCCTTCTTCTCAGAGGGAGAGG ATCAGGTAAACTTCCGAGGATTCATGAGAACATTGGCTCATTTCCGACCCATTGAGGATAACGAAAAGAG CAAAGATGTGAATGGCCCGGAACCCCTCAACAGCCGGAGCAACAAGCTGCACTTTGCTTTCAGACTCTAT GATTTGGATAAAGATGACAAGATCTCCCGTGATGAGCTGTTACAGGTACTGCGAATGATGGTTGGAGTGA ATATCTCGGATGAGCAGCTGGGCAGTATTGCGGACAGGACCATCCAGGAGGCTGACCAAGATGGAGATAG TGCCATATCTTTTACAGAATTCGTTAAGGTTTTGGAAAAAGTGGATGTAGAACAGAAGATGAGCATCCGA TTTCTTCACTAAAGGGCAGAGACCAAATTATTCTTGCTTTCTAGTATTTAAGAACTGAAACTTGTGTCCT CCAGCCCCACCCCATCCTGTCATCCCCTTTTCCCAGAATACTACAGCTCTTGCATGACAACCTGAAGTCT CTCCTGTCCATACAAACTTGCCTTTGGTGTATAAACAGGGCGTTACAGAATGGTACCCTCTGTCTGCTTC TGTTCAGTATCCACCCACCAGGTCCTTAGTTGTCAGTACCATCTTCCCCTGTTAGCTGCTGAGTGCCATG CATCCATCCTGTCTGGGGAAATGAACCAGCCAGCAAAGCTCCCACTGGATGGGGACTATATCCATGCTGC CTTCCATCCCTGCATCGAGCCTCCACCATGTTTCTGTGACCTTGTGTCCTTTTGTCCTTGTGTCTGTTAC TTCCTACCTCTCAGTCATCCAACCTTCTCTCCATTCAGTTTTTCAGCTGCCCTCCTGTCGTTGAGATTCC CTGCATTTTACTTGATTCTGGGCTCTGGGCTCTGCTGTTAAGCCTGTTTCTGATGTGGCAGGATAGTTAG TGGTGTGGTGAGTTATAGCTACCTTCTAATGTGTCTCTGGGACGTTGCCTATCCAGGAGCTTTTCAGCAA GCAGTACTGCTCTTGGAAGCATGTGACTCCCTTGGTCCCCTGTATTGCTCTGTTGTCGAAGGGCTTATGA GCAGACATGTTCTTAAAAGGTGGTCATGGTGAGGCTTGTAGAGCCATGTTTCCTAGGTGGCTTCCCAGGT CTCTCTACCTCTGACTTTTTATTTTCAAACATATTTATGTGGCTTTTCTGGGTACAGTGTTGACATTCAT TAGAAAATATTCTAATGCCTGCCAGTTTGCTTCTCTTTGGAACATTGTCACCACCCAAGTGGCTCTTCTG ACAATGTGGTAGAGGGCCTTCTTGTAGTTTTCTGAACAAGGCTAGGCACCCTGGAGAGCTGGCCACATTT GCTCTACAGCATGGGTCATGCCTCAGTGTTCTGTACACTGGAAGAGTCTTCACATTTCCAAACAGAAAGA CTAGTGTTACAGAAGTGCCTGACTTATCTCAGTCCTAAAGAATTAGATTGTCCTCAGTGTCTTAAAGTTT CGCACAAAACTCTGAATGAATTTTATACTTGGCAAATTTTAATGCTGGACGATGTGCAGTCAGTTCATAG TCCTGGTCCAGGCCTTTAGTGTATTGTCTCATGAGCCTCCTGATGGCGTGACCTTCCTAGCTCCCTGTGT ATACTACGCAGATCCCATAGAAGCATGGGGCCAAGGATTGGTGGCACCAACTGAGGTGACATTTAGAGGT CTTTGAGTGACCCAGCTCAACTTCTCTGCCAATGTCAGCTTGATTTAGATCTCACTGAATCAGGCTGCTT CTGTCCTGTGTCCCTGTGAATCTGCTCTTGGCTGGCTTTCTCTGATGTCGCTTCCTTGCTTGCTTGCTTC GTTGCTTTGGAAGGCTATCCAAGGTGTGTAAACAATTCTTTAGTAAATAAATTGCTATTAACCCAAACAA ACAAAAACACTGCAAAGAGGATGGGTAAAGGTGAGCAACAGGGGAATGGCTGGGATAGATGGCTGGGGCT ACATGAACTGGGGATTCTTTACGAAATATCTGTTAGACTTTGTGATCAAATGATGTGATAAAAGAATTGT ATAAAGGGAATAATTGTCTGATACTGATCTGTTTGAGAAGTATTGAGAAGCTTCTCAATTTAAATATTTA AACTTTCTAAGCTTATGCCCCCTTGGCTGTGTATAGCAAACTGTTTTTATCCACAGTTGTGCCTTATTGT CCCATTAAAGTTGTATTCCTCAATTCATCAATAAATCAATTGTTGTTAAAACAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5720240 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5720240 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7541 same embryo
	EMAGE:7544 same embryo
	EMAGE:7545 same embryo
	EMAGE:7546 same embryo
	EMAGE:7543 same embryo
	EurExpress:euxassay_004666 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source