EMAGE mouse gene expression spatial database

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EMAGE:7545

Kif20a kinesin family member 20A ( MGI:1201682)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004675. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004675_01	euxassay_004675_02	euxassay_004675_03	euxassay_004675_04

euxassay_004675_05	euxassay_004675_06	euxassay_004675_07	euxassay_004675_08	euxassay_004675_09

euxassay_004675_10	euxassay_004675_11	euxassay_004675_12	euxassay_004675_13	euxassay_004675_14

euxassay_004675_15	euxassay_004675_16	euxassay_004675_17	euxassay_004675_18	euxassay_004675_19

euxassay_004675_20	euxassay_004675_21	euxassay_004675_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7545_wholemount_strong.wlz

7545_wholemount_moderate.wlz

7545_wholemount_weak.wlz

7545_wholemount_possible.wlz

7545_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7545_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

homogeneous

strong expression: see section 12 moderate expression: see section 09 10 11

diencephalon lateral wall ventricular layer

moderate

homogeneous

moderate expression: see section 09 10

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 07 08 11 12 13

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 05 06 07 08 14 15 16

midbrain ventricular layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8643
Entity Detected:	Kif20a, kinesin family member 20A ( MGI:1201682)
Sequence:	sense strand is shown >T8643 GAGAAGCGGCGAGGCAGACAGTCTTCGGGTGAGTGAAGTTGGGCTGGAGCCCCGGTTATCAGCTGCTGCT TGCGCGTCCGTAGAAGGCTCCTGTGTCTTCCATAATTCTCTAGGTCTCGTGGTCTTTGGAATAAGGTTGG AAAAGTACCCTTGGGATCCTAAAACACGCTCGCTGGCGGACCTGTAGACCACCTCTGTAGAGCGTGGCCT TCCGTTCTGCTTTAGAACTAGACATAGACTGACTCTGCTTGTCATGTCTCACCGGATCCTTTCTCCGCCA GCGGGCTTACTCTCTGATGAGGATGTTGTAGACTCTCCCATATTAGAATCCACAGCTGCAGATCTGAGAT CTGTTGTCCGAAAGGACCTGTTGTCAGACTGCTCTGTCATCTCTGCCTCTCTGGAGGACAAGCAGGCTCT ACTGGAGGACACCTCAGAGAAGGTGAAAGTTTACCTTCGAATTCGACCATTCTTGACTTCAGAGTTGGAC CGACAGGAAGATCAGGGCTGCGTCTGCATTGAGAATACAGAAACCCTTGTGCTGCAGGCACCCAAAGACT CCTTTGCCTTGAAGAGTAATGAGAGGGGAGTTGGTCAGGCCACTCACAAATTTACCTTTTCCCAGATATT TGGGCCAGAAGTGGGACAGGTGGCCTTCTTCAATCTGACCATGAAGGAGATGGTGAAGGATGTGCTCAAA GGGCAGAACTGGCTCATCTACACCTATGGAGTCACCAACTCGGGGAAAACGTACACCATTCAAGGTACTA GTAAGGATGCGGGGATCCTGCCCCAGTCCCTGGCTCTCATCTTTAATAGTCTCCAGGGCCAGCTTCATCC GACACCTGACCTGAAGCCCTTATTGTCCAATGAAGTAATCTGGCTAGACAGCAAGCAGATTCGACAGGAG GAAATGAAGAAGCTGTCCTTGCTAATTGGAGGCCTCCAAGAGGAGGAGCTGTCCACCTCCGTGAAGAAAC GTGTCCACACTGAAAGTCGGATAGGTGCCAGCAACAGCTTTGACAGTGGCGTTGCTGGGCTGTCTTCCAC CAGTCAGTTCACCAGCAGCAGCCAGCTAGATGAAACAAGTCAATTATGGGCACAACCAGACACTGTCCCA GTAAGTGTTCCAGCAGACATTCGCTTCTCTGTCTGGATCTCCTTCTTTGAGATCTACAATGAATTGCTTT ATGACCTGTTAGAACCACCTAGCCATCAGCATAAGAGACAGACACTTCGGCTGTGTGAGGATCAGAACGG CAATCCTTACGTGAAAGATCTCAATTGGATTCATGTTCGTGATGTTGAGGAAGCCTGGAAACTATTGAAA GTGGGTCGCAAGAACCAGAGCTTTGCCAGCACCCACATGAACCAGCAATCCAGCCGCAGTCACAGCATCT TCTCAATCAGAATCTTGCACCTTCAGGGAGAAGGGGATATAGTGCCCAAGATCAGTGAACTGTCACTCTG TGACCTGGCTGGCTCTGAGCGCTGCAAACATCAAAAAAGTGGTGAGCGGCTAAAGGAGGCAGGGAACATT AACACTTCTCTGCACACCCTGGGCCGCTGTATTGCTGCCCTGCGACAGAATCAGCAGAACCGGTCAAAGC AGAACCTGATTCCTTTCCGTGACAGCAAGTTGACTCGTGTGTTCCAAGGCTTCTTCACAGGTCGAGGTCG TTCCTGTATGATTGTCAATGTGAATCCCTGTGCATCTACATATGACGAGACTCTTCATGCAGCCAAATTC TCAGCCCTAGCCAGCCAGCTTGTGCACGCCCCACCTGTGCATCTGGGAATCCCATCCCTGCATTCATTCA TCAAGAAGCACAGTCCTCAGGTTGGCCCTGGCTTAGAGAAAGAGGACAAGGCCGACTCAGACCTTGAGGA CAGTCCTGAAGACGAAGCTGACGTCTCCGTGTATGGCAAGGAGGAACTACTCCAGGTGGTGGAAGCCATG AAAGCGCTACTTTTAAAAGAACGACAAGAAAAGCTGCAGTTGGAGATACAGCTCCGAGAGGAAATTTGCA ACGAAATGGTGGAACAGATGCAACAGCGGGAGCAGTGGTGCAGTGAGCGTTTGGACAACCAAAAGGAACT GATGGAGGAACTGTACGAGGAGAAACTGAAGATCCTGAAGGAATCACTGACAACTTTCTACCAAGAGCAG ATCCAGGAGCGGGATGAAAAAATTGAAGAGCTAGAAACTCTGTTGCAGGAAGCCAAACAGCAGCCAGCGG CACAACAGTCAGGGGGGCTGTCCCTCCTCCGGCGGTCACAGAGGCTGGCAGCGTCTGCCTCCACTCAGCA GTTCCAGGAAGTTAAAGCTGAACTAGAGCAATGCAAAACAGAGTTAAGCTCTACCACTGCAGAGCTGCAC AAGTATCAGCAAGTGTTAAAACCGCCACCCCCAGCCAAGCCTTTCACCATTGATGTGGATAAGAAGTTAG AGGAGGGCCAGAAGAATATAAGACTGCTACGGACAGAGCTCCAGAAACTAGGACAGTCTCTGCAGTCAGC AGAAAGAGCCTGTTGCCACAGCACCGGAGCAGGAAAACTTCGTCAAGCATTAACCAACTGTGATGACATC TTAATCAAGCAGAACCAGACCCTGGCTGAGCTGCAGAATAACATGGTGCTAGTGAAACTGGACCTTCAGA AAAAGGCAGCCTGCATTGCTGAGCAGTATCACACTGTGCTAAAGCTCCAAGGCCAAGCCTCTGCCAAAAA GCGGCTGGGAGCAAACCAGGAAAACCAGCAACCAAATCATCAACCCCCAGGAAAGAAACCATTCCTGCGA AACTTACTTCCCCGAACACCTACCTGCCAAAGCTCAACAGACAGCAGCCCTTATGCGCGGATCTTGCGCT CACGGCACTCTCCTTTACTCAAATCTCCCTTTGGCAAAAAATACTAAAGTTGTGGGGATGAAGATGCATG GTTGTTGCCCTGAGGTTGGTCAGCTGCTTAGCTGATAAGTCTCTTTTAAGCTTCAGCATACACCCAGAAC TGTATTCAGAATTATTGTATTATAATCACCTATGTAATCTTGTTTTTTTTTCATGCACAAATATTATATG GAATAAAGACATTGTTTATGCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5720408 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5720408 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7542 same embryo
	EMAGE:7541 same embryo
	EMAGE:7544 same embryo
	EMAGE:7546 same embryo
	EMAGE:7543 same embryo
	EurExpress:euxassay_004675 same experiment
	MGI:4825756 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source