EMAGE mouse gene expression spatial database

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EMAGE:7552

Prmt6 protein arginine N-methyltransferase 6 ( MGI:2139971)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004590. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004590_01	euxassay_004590_02	euxassay_004590_03	euxassay_004590_04

euxassay_004590_05	euxassay_004590_06	euxassay_004590_07	euxassay_004590_08	euxassay_004590_09

euxassay_004590_10	euxassay_004590_11	euxassay_004590_12	euxassay_004590_13	euxassay_004590_14

euxassay_004590_15	euxassay_004590_16	euxassay_004590_17	euxassay_004590_18	euxassay_004590_19

euxassay_004590_20	euxassay_004590_21	euxassay_004590_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7552_wholemount_strong.wlz

7552_wholemount_moderate.wlz

7552_wholemount_weak.wlz

7552_wholemount_possible.wlz

7552_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7552_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus mantle layer

weak

regional

weak expression: see section 07 08 09 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7610
Entity Detected:	Prmt6, protein arginine N-methyltransferase 6 ( MGI:2139971)
Sequence:	sense strand is shown >T7610 CCAAGATGTCGCTGAGCAAGAAAAGAAAGCTTGAGTCGGGGGACAGCGGAGGCGCCGGCGCCGGAGGGGA GGGAGCCGAGGAGGAAAATGGCGGGGAGCAGGAGGCGGCCCCGCCACGACCCCGGAGGACCAAGAGCGAG CGGGACCAGCTGTACTACGAGTGCTACTCCGACGTCTCGGTCCACGAGGAGATGATCGCCGACCAGGTCC GCACCGAAGCCTACCGCTTAGGCATCCTGAAGAACTGGGCCGCGCTGCGAGGCAAGACGGTGCTGGACGT GGGCGCGGGCACCGGCATTCTCAGCATCTTCTGTGCCCAGGCCGGGGCACGGCGCGTGTACGCGGTGGAG GCCAGCGCCATCTGGCAACAGGCCCGGGAGGTGGTGCGGCTCAACGGGTTGGAGGACCGCGTGCACGTCC TGCCGGGCCCGGTGGAGACCGTGGAGCTGCCGGAGCGAGTGGACGCCATCGTCAGCGAGTGGATGGGCTA CGGACTTCTGCACGAGTCCATGCTGAGCTCCGTGCTCCACGCGCGGACCAAATGGCTGAAGGAGGGCGGT CTCCTCCTGCCAGCTTCCGCGGAGCTCTTCGTGGCCCCGATTAGCGACCAGATGCTCGAGTGGCGTCTGG GTTTCTGGAGCCAGGTGAAGCAGCACTATGGCGTGGATATGAGCTGCATGGAGAGCTTCGCCACGCGCTG CCTCATGGGCCATTCGGAGATCGTGGTGCAGGATCTGTCCGGAGAGGACGTGCTGGCCCGGCCGCAGCGC TTTGCCCAGCTCGAGCTGGCCCGAGCCGGCCTGGAGCAGGAGCTGGAGGCTGGTGTGGGCGGGCGCTTCC GCTGCAGCTGCTATGGTTCCGCGCCTCTACATGGTTTCGCCGTCTGGTTTCAAGTGACCTTTCCCGGAGG GGACTCGGAGAAACCTCTGGTGCTGTCCACCTCGCCTCTTCACCCGGCCACCCACTGGAAGCAGGCGCTC CTCTACTTGAACGAGCCGGTGCCGGTGGAACAAGATACGGACATTTCCGGAGAGATCACCCTGCTGCCCT CCCCGGACAACCCCCGGCGTCTGCGCATACTTCTGCGCTACAAAGTGGGAGACCATGAGGAAAAGACCAA AGACTTTGCCATGGAGGACTGAGCGTTGCCTTTTCTCCCCCTCCACCTTCTTCGGAAGGTCCGAAGAGAA AGGGAGATCGCCTATGCAAGTTGGGGACATAGCTGTCTCTTTTACCTCACCGCTCCAGACTTCATTTGAG GACATTCTTCTGGCAGTGTTTATTTTAAAAGCACTGGCAGTATCAACATGGCTATACATGAAGAGCAATG TATGCCACAGGGTACCAGATGATCCAAAGGAGCCCCACAGCCTTTGGGAGGAAGACACCTGGGATGGATG AAAGCCTGAGGTGTGTGGACACCCAGGCACCATCTGATGTGTTATGTGGATTGAGACTGATAACATGAAG CCCTTAAGGCTGCAGGAGGGGATTATCAGACCCTAGAAAGAGCATCATGATAAAGTTCCACCTACCTCCC TCCTCATACCATTTATTTAGCCCTCTAAAAATACCCAATCTTGTCATTTTATTAAACCAATGAGCACAGT TGCTAGAAATGCATTCTAATAGTCATTGTGATAGGTATTTGTAATTACAAGCTAATGTGAACCCTGCTCA TAAATAGATGTCTACCACTAAAGAAAGTAAAGGCCATTTGTTAGGACCTGGATGGTTTTAGTGTCAGAGA AGATGGATACTCTGTTTTAAACACTAAGTAGAAAGGTTTCTAGAGATGCTGATATACTTGACTAAAGTTC CATACATAATTCTTGCTTGTGAAATGGGACCATACATTGTGCAAGTGGGGCTCGGAGCCCAGTATGGAGC TGAGGATGACTTTGGACTCCTGAAGCTCCTGCCTCCACCTTCCAAATGCTGGAGCTAATAGCACTGTACC ACTGTGCCCAGCATAGTTTTTGACAAAACCTTACGCACGCACAACTCTGACTTTCTAATTGTAAACAAAA ATTCACTCCTGTTATACTAATCCTGCATTCTTCAGTTAAAAGCTGGAAACAACATGCCACCTTAAAGGTA ACAGGGGTTTTATTATTTGGTGATGAAGGGGCAGTGTTACATACTAGAAAGTCTAGAGCTGCCCAGAAGA AGCATCTGCAGTGTATAACTTCTTGAGTTTGACGTAGTAGGCACTCATTTGGACAGCCCCATTGTTTATT TCCTTAACACTAATCGAATGTGACACTTTCTGGGGTCCCCAGAAAGCTGAATCCATGACAGTGGCAAAGA CAAGTGAACTCCTTGTCATTTGGTCATTTGGGCAGAGAGACGTGGATGTAAAAGATAAAATGGAGTTCAA GGTGACTGAAAGGCACCTGCTGCAAACAGCTGCCTGGCATCCTGTTTACTTCCAGCCCTGCTGTTATCCC TACAGTTTACCTAGTGCTGAGGAAAGATGGCTCCTAAGGAGCCTTTACTAACAGGCCCCACTCCTCCACC TGCTCATCTCTGTAGATGGATCTCCATTAATTTCATTATCAATAGCTACAGAATATCTAAGCACTATAAT GAATGAGCACATCTGCCCCCTCAAAGAACTAGGATACAGTGGGAACAATAAAACAACATCAGTGATTTTC ATGCATCCATAAGCCTGGAAATCCTTTGCAGCGCTTAGCTCCACTGAGCAAGGATGGAAAGGCAGCTGTC ATCCTTTAAGCCTCTCTGAGGATCAAAAGTCGTGTTCCTATCATCAGGAAGATTGTGAACACCTGAGAAA CTTTCCCATCTGATGGTGCAAGCTTAGACAAAAGATGGTCTGTTTCATAATAGACCAAGTATGTTAAATA GGTGATCCTCAGCTACTAAAATAAAAACTAAGCCAAATTTATATTCAGGGGTGTGTGTGTGTGTGCGCAC GCGCGCAGGTGTCTTCCTCAGCTTCCTATGTGATTTCCATGCTTCAGTTCGTTGATGATTTATCCTCTTG TAATAAATATTACATGTATTTTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30436952 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30436952 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7554 same embryo
	EMAGE:7555 same embryo
	EMAGE:7553 same embryo
	EurExpress:euxassay_004590 same experiment
	MGI:4827432 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source