EMAGE mouse gene expression spatial database

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EMAGE:7553

Cep164 centrosomal protein 164 ( MGI:2384878)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004608. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004608_01	euxassay_004608_02	euxassay_004608_03	euxassay_004608_04

euxassay_004608_05	euxassay_004608_06	euxassay_004608_07	euxassay_004608_08	euxassay_004608_09

euxassay_004608_10	euxassay_004608_11	euxassay_004608_12	euxassay_004608_13	euxassay_004608_14

euxassay_004608_15	euxassay_004608_16	euxassay_004608_17	euxassay_004608_18	euxassay_004608_19

euxassay_004608_20	euxassay_004608_21	euxassay_004608_22	euxassay_004608_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7553_wholemount_strong.wlz

7553_wholemount_moderate.wlz

7553_wholemount_weak.wlz

7553_wholemount_possible.wlz

7553_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7553_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7611
Entity Detected:	Cep164, centrosomal protein 164 ( MGI:2384878)
Sequence:	sense strand is shown >T7611 CCGCGACCATGAAAGGAAATTACAAGACTTAGAGGTGGAACTCTCGTCCAGAACTAAAGATGTCAAGGCC AGACTAGCTCAGCTGAATGTTCAGGAGGAGAACATACGGAAAGAGAAGCAGCTGCTCCTGGATGCGCAGA GGCAGGCTGCTCTGGAGAGGGAGGAAGCCACAGCCACCCACCAGCACCTGGAAGAAGCCAAGAAAGAGCA CACACACCTGCTGGAGACGAAGCAGCAGCTGCGAAGGACCATCGATGACCTGAGGGTCCGGCGGGTGGAG CTGGAGTCCCAAGTGGACCTGTTGCAGGCGCAGAGTCAGAGATTGCAGAAGCACCTGAGCAGCCTAGAGG TCGAAGTACAAAGGAAGCAGGATGTCTTGAAAGAGATGGCAGCTGAGATGAATGCTTCCCCACACCCGGA GCCAGGGCTCCACATTGAGGACCTGAGGAAATCCCTTGACACAAATAAGAACCAAGAGGTGTCCTCTTCT CTCTCCCTGAGTAAGGAGGAGATCGACCTGTCTATGGAGAGTGTCCGACAGTTCCTCTCTGCTGAGGGTG TAGCTGTGCGCAACGCTAAGGAGTTCCTGGTGCGCCAGACACGCTCCATGCGGAGGCGACAGACAGCTCT GAAAGCCGCCCAGCAGCACTGGCGCCATGAGCTGGCCAGTGCCCAGGAGGTAGACGAAGACCTGCCAGGC ACCGAGGTCCTGGGAAACATGCGCAAGAACTTGAATGAGGAGACCAGGCACCTGGATGAGATGAAGTCAG CCATGCGCAAAGGCCATGACTTGTTGAAGAAGAAAGAGGAGAAGCTGATCCAACTGGAATCTTCTCTTCA GGAGGAGGTCCCTGACGAGGACACTCTGAAAGGATCCTCTATCAAGAAGGTGACCTTTGATCTCAGTGAC ATGGACGATTTGAGCAGTGAGAGTCTTGAGTCCAGCCCCGTGCTGCACATCACCCCGACCCCGACCTCTG CTGATCCCAACAAGATCCATTACCTGAGCAGCTCCCTTCAGCGAATCAGCAGTGAGCTAAACGGCGTGCT GAATGTGCTGGGAAGCCTCAACTCCCAGCCGCCGCCCCAGGGCCTCGGCAGCCAGCCCCCGCCTCCACTC TTCACCTCCTCACTTCGGTCCTCCAAGAATGTCCTTGACCCTGCCTACTCCTCCCAGGCCAAGCTCTCCT CCTTATCTTCCATCACACCCATGTCCACCCAGTGGGCCTGGGACCCAGGGCAGGGCACCAAGCTGACCTC TTCCTCCTCCTCTCAAACTGTGGATGATTTCCTGCTGGAGAAGTGGCGCAAGTATTTTCCCTCTGGCATC CCGTTGCTCAGTGGCTCTCCTCCCCCACCGGAGAACAAGCTGGGTTATGTGTCTGTAAGTGAGCAGCTCC ACTTCCTGCAGCGCTCCCACCCTCGAGTCCCCAGGACGGATGGTGTCAGCATTCAGAGCTTGATCGATTC CAACCGGAAGTGGCTGGAACATTTCCGGAACGACCCCAAGGTACAGCTTTTCTCCTCGGCGCCCAAAGCA ACAACCACATCGAACTTATCGAACCTCCTGCAGCTGGGCCTGGACGAGAACAACAGACTGAATGTGTTTC ATTACTGAACCCCGTGTGGGGCGGGGCAGGGCGGCCTCCTCCTCTATTACAAGTGCCTGAGGAGCCGCCA ACATTTTATTTCTCTCATCAAGCATGTCCTCCGTTGTGAACCCCACCGAGGACTTAGAGGAACCACCGGG TTAGATAGGTGGAGAGTACGGCTGTAGACGGCCACGCACGCTTCAACCATGGACTGTCCTGAGCAGAAAC ATGTGGACATATGCGAGCTTCTGCACCAAAGTGGCACTGAGATGTGGGCTGCTGAAGACTTCAGGGGTGT GGACACCTTAGTTAAAGCGACTGCTATGGGAGGCTTAGGGATCCTCCTTGGAGGTTGGCCCCCTGCTGAG GATGTGGTCCTTTATTCACATGGCCTCCTAGCATGGCATGCCCTGGCACTTTGGCTGTGCTGGGTCTGTG CCCCTTTCGGATACCTCAGCCCCGACAGGATCATACCCGTCCCTCTCTTTTTGCCTTTTTTTTTCTTTGT AAATGTCTTCTGACTGTCACAAGTTTGGGCTCAGCTGCCTGAGCCCGGCTGGATGTTGCTAGAGGGGCCC CGAGGGATGTTCTGTCTCCTTCCCCTCGGCTGGCACCGTGATTTCCTCACGATTCTCAGGAGCAGCCCTC TCCCCCCTTAGGGTGCTCTGTGGAGCTCTTCCTTGCCCTCAGGTTTCAAGCACCTATCTTTCCAGGAAGC CAAAAAAGCCATAGGGAAAGAGGGGAATGCCTTCTGCCCCATCTTGATATTTCTCTTCTTTAACTCGAGT GTGTCTATGAGCCCTATTTCCATCTGTCCTTCTATCTGGGCCGCCCCTAGCCCAGTCCAACTAGCAACTC TGTCCAGAGCTCAGTGGATGGAACTGATTCCAGCCTGGCACCAGCAGGGACCATCTTGGGTCAGGTGCTT CTGGCTAGTGCTTCTGGCTCAAGCCTCCTCGGCCCAACTTCCCCTCAGCGGGCTCTGCAGGAGCTGCAGG TGATGCTCTCAGCATCTGTCTCCTTGTTTAAACAAAATGCCCTCATTTTTCTTGTCTTGAGAAGCTGCGG GCTGCCTCTACCCCCGCAGCCCACAGGGTCTCACCGATTTATTGTTCGTTTCTTTGCTGTGGAATAAACC TCGAGCAGCCAGTTGGCTTTTTACCCTTTAGATATTTTTTAGTCATCGGCTGACCACACACAGATAAAGT CCGTTTTGTTACTTCTTCCACTCTGTGTGCCTTGAGAACCGCTCATGTGGTACAACCAATTCTGGTGACA ATCCTGTGATTTTTTTTTTTTTTGCACCTATTAAATCTCTGTAAGTCAATTTTCAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30437501 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30437501 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7552 same embryo
	EMAGE:7554 same embryo
	EMAGE:7555 same embryo
	EurExpress:euxassay_004608 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source