EMAGE mouse gene expression spatial database

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EMAGE:7556

Art4 ADP-ribosyltransferase 4 ( MGI:1202710)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004604. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004604_01	euxassay_004604_02	euxassay_004604_03	euxassay_004604_04

euxassay_004604_05	euxassay_004604_06	euxassay_004604_07	euxassay_004604_08	euxassay_004604_09

euxassay_004604_10	euxassay_004604_11	euxassay_004604_12	euxassay_004604_13	euxassay_004604_14

euxassay_004604_15	euxassay_004604_16	euxassay_004604_17	euxassay_004604_18	euxassay_004604_19

euxassay_004604_20	euxassay_004604_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7556_wholemount_strong.wlz

7556_wholemount_moderate.wlz

7556_wholemount_weak.wlz

7556_wholemount_possible.wlz

7556_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7556_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

viscerocranium

strong

regional

Expression in the turbinate bone.

aorta

moderate

regional

moderate expression: see section 10 11 12 13

heart ventricle

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09

liver right lobe

strong

regional

strong expression: see section 10 11 14 15 16 17 18 moderate expression: see section 12 13 weak expression: see section 19 20 21

trachea

moderate

regional

moderate expression: see section 08 09 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7689
Entity Detected:	Art4, ADP-ribosyltransferase 4 ( MGI:1202710)
Sequence:	sense strand is shown >T7689 GGCCCTTTCGCGGAGACTTCAACACTGCAAAGGCGCTAGACTTCTACCTTGGAGGAAACTGTCGGTTGTT GAGGTTGTTGATATTCAACAGAAGTCACAGTTGCGGCAGGAGCAGAACCTCAGACACAGCCGGAAAAGAA AATGCCAAAGGAGGACTCTAGAGCGTATTCTGGGACGAGGCTTTCAGAATAAAGAAGTGTTGCTGACACA AACCACCAAGCTGCCCACTGACCAGTAGATCTAAGAGAACGTGTCTACCAAGTGCTACAGACAGGAGGAT GGCGCTGTGGCTTCCAGGAGGGCAGCTCACCCTGCTGCTACTGCTCTGGGTCCAGCAGACACCCGCGGGG AGCACTGAGGCTCCTCTTAAGGTCGACGTGGACTTGACACCTGATTCCTTTGATGATCAATACCAAGGCT GTAGCGAACAGATGGTGGAGGAGCTAAATCAAGGAGACTATTTCATAAAGGAAGTAGATACTCATAAGTA TTACTCCAGAGCATGGCAAAAAGCCCACCTAACCTGGCTGAACCAGGCAAAGGCCCTCCCGGAGAGCATG ACCCCGGTGCATGCTGTGGCCATTGTGGTTTTCACCCTGAATCTCAATGTGAGCTCTGACTTGGCCAAGG CCATGGCCAGGGCTGCGGGATCTCCTGGGCAGTACAGTCAGTCATTCCACTTCAAGTATCTGCACTACTA CCTCACCTCAGCCATCCAGCTGCTGAGGAAAGACAGCTCTACAAAGAACGGCAGTCTGTGTTACAAGGTG TACCACGGGATGAAGGATGTGAGCATTGGAGCCAACGTAGGTTCCACCATTCGATTCGGCCAGTTCCTCT CTGCCTCCCTGCTAAAGGAAGAGACACGGGTGTCTGGAAACCAAACCCTGTTTACCATTTTCACTTGTCT GGGTGCCTCTGTGCAAGACTTCTCTCTCAGGAAGGAAGTCTTGATCCCCCCCTATGAGTTATTTGAAGTC GTAAGTAAGAGCGGCAGCCCCAAAGGAGATTTGATAAACTTGCGGTCTGCTGGGAACATGAGCACGTATA ACTGCCAGCTGCTAAAAGCTTGCAGCAAGAAGTGCGCCCCTGCTCCAGTGGTTATCGGCTGCCTCTTTTT GGTCACTGTTGTTATCTCGTCCAAAAGCAGAGCGCAAAGGAATCTACTGGCTCCTTTTTAAAGGAGCTGC TTAAATTTGATGCTCCTATTTGTGTTACCAAAGTCTGAGGCCCACACTTCCCACAATACCACAGAAGATG TGCAAGAAAAGTTCATGGGGGGGGAGAGGGGGCGGGGACAACCCTAAGTTCAAATCTAGGCCTAAATAAG GCAGAAGACTGTCAGAAATCCCTGCCTAAAAGGATTTAGGCTACTTTTTCCTGTTGCTGTCAGCAAAACC AATCACAGAAGCTCAAACAAAGTTCAGGATGAAAGCTTTGCTTCCACTTATAAACTTACAGAACATCGTA GACCAGAAGTGTTTGGTCCCAGCAGCACCAGGCACTACAGAAGCAGATACCAGGCAGACTATCAAATCTC AAGGCCTGCCCCTTGTGACTCACTTCCTCCAGCAAGGCTCCACCTCCGGAAGACTCTACAGCCACGAAAA ATAACACCACCAGAAGGAGACAAAGATCTCAACACATGAGGCACATTTAACCTTCAAACCACAGCAAGGT CTTAGTGTTCAGCGCTCTCTTCCAGGAGAGGAGATGGGAGTCCTGACAGACAAGGGCAGAACATCCCTGC TCTGTGAAGGTTTTTCCTACCCAGACCCGTCTTTGCTCTGCCCTCCTCAGGAACCTCACAAAGGACCACT CTCCTGCAGCCAGCCAACCAGAACTGAAAAGGGCCTTCAGCACACTGCTACTGAAAGCCATGCATGGCGT CTTCCTTGCCTGTCCATTTGCCCTTCCCAGTCTCAGGGCCTTGGTCTTATTCAGTGAAGACACCCAAGGA TACCACTTGCCCATTACTTGGCCAGAGATCCATGACAAAATGACAATCTTGTGGCAGCATATGAGACAGA ATTAGGATACGTTTTCTAGATTAGTTTTGGGAGCGCAGCATTCAGAATCTATTGTGAGTGATTATAAAAG CTGAGCACATGGCACGCTGCGTTGCCTGTAGCTGCCTCGCCACCAAGAGATAAGAATTTGCCTCCCTAAG AAACTAATTCAGTGGAGGCAGTGCTAAGAAACAGAGAGAAGATAGGCAAGATGAGCCTCTAGCGCCAAAC TGGGCCTGAACCAGATAGTCAGCTTCATCATTTTCATGGATTAATAAATGATATCTGTGCTAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6306234 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6306234 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7558 same embryo
	EMAGE:7559 same embryo
	EMAGE:7557 same embryo
	EurExpress:euxassay_004604 same experiment
	MGI:4823264 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source