EMAGE mouse gene expression spatial database

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EMAGE:7558

Dcdc2a doublecortin domain containing 2a ( MGI:2652818)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004609. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004609_01	euxassay_004609_02	euxassay_004609_03	euxassay_004609_04

euxassay_004609_05	euxassay_004609_06	euxassay_004609_07	euxassay_004609_08	euxassay_004609_09

euxassay_004609_10	euxassay_004609_11	euxassay_004609_12	euxassay_004609_13	euxassay_004609_14

euxassay_004609_15	euxassay_004609_16	euxassay_004609_17	euxassay_004609_18	euxassay_004609_19

euxassay_004609_20	euxassay_004609_21	euxassay_004609_22	euxassay_004609_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7558_wholemount_strong.wlz

7558_wholemount_moderate.wlz

7558_wholemount_weak.wlz

7558_wholemount_possible.wlz

7558_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7558_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 09 weak expression: see section 10 13 14 15

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 05 weak expression: see section 06 07 08 09 10 11 13 14 15 16 17

4th ventricle lateral recess

moderate

regional

moderate expression: see section 02 03 04 05 19 weak expression: see section 06 07 08 09 12 13 15 16 17 18 20

rest of cerebellum mantle layer

strong

regional

strong expression: see section 02 03 18 19

urinary system

strong

single cell

strong expression: see section 02 03 18 19

kidney calyx

moderate

regional

moderate expression: see section 05 07 08 13 14 15 16 weak expression: see section 04 06

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7688
Entity Detected:	Dcdc2a, doublecortin domain containing 2a ( MGI:2652818)
Sequence:	sense strand is shown >T7688 CCGGCTCGCGCAGGGGTTTAAAGCCCGAGCGGCGTGGGTTCCCAGACTGCACACCTGGATGCTTCGGGGC GGGGGAGACAGGTGTGCGAGCCTGCGAGCGCTGATTGGCCGTCCGTGCCGCGCCTTCCTTGAATTGCTAC CTGCTAGGGCTGGCCCCGCAGCCCTTCCTCACCTGCCGCTAGCTTTCCTTGTCCCTGCAGGAGGCGGTCC ACGAGCTGCTGTTGCAGGCCGGTGGAGTGCTTGCGGGCTCGGCGTCCCGCGGAGAAGATGAACGGTCCCA GCTCCAGGTCCAGCCACCTGTCGCAGCCGGTGGTGAAGAGTGTGCTGGTGTACCGCAATGGGGACCCCTT TTTCGCCGGGCGGCGTGTCGTCATTCACGAGAAGAAGGTGTCCAGCTTCGATGTCTTCCTCAAAGAAGTG ACTGGCGGTGTGCAGGCGCCTTTTGGGGCGGTTAGGAACATCTACACCCCGAGGACGGGCCACCGCATCC GAAAACTGGACCAGATAGAGAGCGGGGGCAACTATGTGGCTGGGGGCCCGGAAGCGTTCAAGAAACTCAA TTACTTGGACATAGGCGAAATCAAGAAAAGACCAATGGAAGCTGTGAATACCGAGGTGAAACCTGTCATT CACAGCAGGATCAACGTGTCCGCCCGCTTCAGGAAGTCGCTGCACGAGCCCTGCACCATTTTCTTGATTG CAAATGGAGACCTCATAAGCCCGGCTTCTCGCCTCCTCATCCCTAAAAAAGCTTTAAATCAGTGGGATCA TGTACTACAAATGGTCACAGAAAAGATAACTCTCCGGAGTGGGGCTGTCCACAGGCTATATACGTTAGAA GGAAAGCTGGTTGAGAGTGGAGCAGAACTGGAGAATGGGCAGTTCTATGTGGCCGTCGGCAGAGATAAGT TTAAGAGACTACCGTACAGTGAGTTACTTTTTGACAAGTCAGCAATGAGAAGGCCTTACGGTCAGAAAGC GTCTTCACTACCTCCCATGGTTGGATCCAGGAAGTCTAAAGGGAGCGGAAATTATCGGCAATCCAAGTCA ACCATCGGGTCTAGTGATAACTCATCTCCTCAGCCTCTGAAAAGGAAAGGGAAGAAAGACTCAAACTCAG AAAAACCCACGAAAGTGAAACAGAGTGTGAAGTCGAAGACTTCCCACCAAGCAATCCCAGACAATGGATG GCTCATAAAAGTGGAGAGAGATACTTGCCTCAGGCCACAGCTGGATGGAAACAGAAATAGAGTGACTGCT TTGCCTCCCTACCCATGGTCCATCCACACAAGACATATGTGGGATGCTCCGGGTGAAGACAGATGGAAAA AGGTCACCAATAAAGCCCAGCCGACGTATGGACACTCCATGTGACATGAAGAGTCATGTGGGATCCACAG ATGCAGGGCAGCGCTTTAGAGGATGCCACCCAGATGCCTAAAGCGTTAGATAAAGACAAGTTATCTAAGG TCAAAAGAGAAATCAAAATGATCTGACAGCCTGGTCAGGAGGCCGAGACTGAGGCTGGGGCTGTAGGATC TTAAGTTCAAGGCTAGGCTGGAGAGTTTAGTGAGTCCCTGTTTCAAAATATAAGAAAGGGCTGAGGATAC AGCTCAGTAGTAGAGTACTTGTCCTATCTGCACGAATCCCTAGGTTCACTCTCCAGGGCTGCATCAGGCA AAAGCACGGGATAAATGCACGGCTCCCCGGAGAAGAAGGGGCTCCAATCTCCGGGTTCTCACTGTGGAGA TTAGCCAGCAGTCAGGCTTGCTGGGCTCCATCCTCCTGCCGTTAGCTAAGAGGTCCACTTTCTCCCTCCC TCCCACTGTCTCATGGTCTTCCTCCTAACTGTGAGCTCATGCTGTAATTTTTCCTCACTACTTTGCATGT CCATAGCCTGTAGTATTCCTTTCAGAGATCAGTCATCAGGGAGGTCAGGATGAAATATAGGTGGCAGGTA TTACTGGGTGCCTATTGTATAGCAAGAAAGACAGAAGATTAACTGAGCTGGGAAAACAGGCCATTTTTGA AAGCTAAAAACAACAGAGCCTTTATTTTGATAACATGTATTGCGCTTTTAAAGCCCCTGAGACTGTGTAC GCACTGTGGCTTACTAGTGCTTTGTTGAGCCAAGATTTTACTCTTGTTTAGGTTGACTGGGAACTCACTA TGCAGTCCAGGCTGGTCTCAAACTCATGGTAATCCTCCTGCCTTAGCCTTCTGAGTGCTGGCATGAACTC TTGTATCTAGTTTCTTTTGAAAAAATTCTCAACGCTAGCCTAGGTCTAATAGAATCAAGGTGTTTAATTA ATATATATTGGCCAAATAAATGGTGGAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6306251 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6306251 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7556 same embryo
	EMAGE:7559 same embryo
	EMAGE:7557 same embryo
	EurExpress:euxassay_004609 same experiment
	MGI:4824246 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source