EMAGE mouse gene expression spatial database

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EMAGE:7610

Alkbh5 alkB, alkylation repair homolog 5 (E. coli) ( MGI:2144489)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004684. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004684_01	euxassay_004684_02	euxassay_004684_03	euxassay_004684_04

euxassay_004684_05	euxassay_004684_06	euxassay_004684_07	euxassay_004684_08	euxassay_004684_09

euxassay_004684_10	euxassay_004684_11	euxassay_004684_12	euxassay_004684_13	euxassay_004684_14

euxassay_004684_15	euxassay_004684_16	euxassay_004684_17	euxassay_004684_18	euxassay_004684_19

euxassay_004684_20	euxassay_004684_21	euxassay_004684_22	euxassay_004684_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7610_wholemount_strong.wlz

7610_wholemount_moderate.wlz

7610_wholemount_weak.wlz

7610_wholemount_possible.wlz

7610_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7610_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8681
Entity Detected:	Alkbh5, alkB, alkylation repair homolog 5 (E. coli) ( MGI:2144489)
Sequence:	sense strand is shown >T8681 AGAGCTTTAAAGTGCGGGCCGGGCCGGGCGTCTGAGGGTCCGACCGGGAGTCTGGCGGAGTCTTGGCCGA AGCCCTCCGCGGCATGAGGCGCTGCCGGCGCCTTTTTCCCTCAGGACGCGGAGAAGGTGGAGGAGGAGGA AGCCCCGCTGTAGCTGGTGCTGCATGACCGACCGCGGCTGAGGTTCGACCCCTTCCCGGCCCCTCTACGC CTACCCCTTTGGTCCCTTGAGCCACGCATGGGGGCCTGGCACTGAGGACCCTCGCTTCCCTTTCGGGTCC CTTTCGGGTCCCCTGAGAGCCATGCCGGACCGCCGCGGAGGACCCTAGAGTTGAGTTGGCGGGGGCCATG GCGGCCGCCAGCGGCTACACCGACCTGCGGGAGAAGCTCAAGTCCATGACGTCCCGGGACAACTACAAGG CGGGCAGTCGGGAGGCCGCCGCCGCTGCGGCCGCCGCCGTGGCTGCCGCTGCCGCTGCCGCGGCCGCCGC TGAGCCTTACCCGGCGTCCGGGACCACCAAGCGGAAATACCAGGAGGACTCGGACCCCGAGCGCAGCGAC TACGAGGAGCATCAGTTGCAGAAGGAGGAAGAGGCGCGCAAGGTGAAGAGCGGCATCCGGCAGATCCGGC TCTTCAGTCAGGATGAGTGCTCCAAGATCGAGGCCCGCATCGATGAGGTGGTGTCCCGCGCCGAGAAGGG CCTGTACAACGAGCACACGGTGGACCGGGCCCCCCTGCGCAACAAGTACTTCTTCGGCGAGGGCTACACG TACGGGGCCCAGCTGCAGAAGCGCGGGCCGGGCCAGGAGCGCCTCTACCCGCCGGGCGACGTCGACGAGA TCCCGGACTGGGTGCATCAGCTGGTGATCCAGAAGCTGGTGGAGCACCGCGTCATCCCCAAGGGCTTCGT CAACAGCGCGGTCATCAACGACTACCAGCCCGGCGGCTGCATCGTGTCCCACGTTGACCCCATCCACATC TTCGAGCGCCCCATCGTGTCCGTGTCTTTCTTCAGCGACTCGGCACTTTGCTTCGGCTGCAAGTTCCAGT TCAAGCCCATCCGGGTGTCGGAACCTGTGCTTTCTCTGCCGGTGCGCAGGGGGAGCGTGACTGTGCTCAG TGGGTATGCTGCTGATGAAATCACTCACTGCATACGGCCTCAGGACATTAAGGAACGCCGGGCGGTCATC ATTCTCAGGAAGACAAGATTAGATGCACCGCGATTGGAAACAAAATCCCTGAGCAGCTCCACATTGCCAC CCAGCTATGCTTCAGATCGCCTGTCAGGAAACACCAGAGACCCTGCGCTGAAACCCAAAAGGTCCCACCG CAAGGCAGACCCTGATGCTGCCCACAGGCCCCGGATCCTGGAAATGGACAAAGAAGAAAACCGGCGGTCT GTGCTCCTGCCCACACACCGGCGGAGGGGGAGTTTTAGCTCTGAGAACTATTGGCGCAAATCCTATGAGT CCTCGGAAGATTGCCCAGAGGCAGCCAGCAGCCCCACCCGCAAGGTGAAGATGAGGAGACACTGAGGCTC TGTGCCCTCTTGGGACTCTGGCTAATCCTGATGTAGCTACCCCATCTTCTGTTCCGTTTGAGTGTTTTTT TTCTTTGTTATTTTGGTCCTATATACGTTTTCCTTGCTTTGTTGACCATTAAGGCTAAAGAGCGAATTGG TAAAAGACCTGGGTTCTTTTGTTCTTGGCTTTCCTCCTTGATGGAAAAAGCTGTTGGCTTCTGTAGAGAA TTGTTCACACCAGAGTAGCCAGTAGAGACAATGGGGACAGCTGTCTTCAAGTGGAAGTTTATTGAAACAC ACAAAATGTTTTGGTTATGAAATTCTAGTTTGGGCTTTAAAATGTAAATCTTTGTAGTGTTTTAAACAAA AACTATTCTTTTGCCAGCCTTTTCTTCCACTGATGCTCATGCTTGGATGAGGTCTCTTGGAGCCATACAG GTCCTGCATTGGGTCTGCTGTGCCCATACATCTCACCTGCTGTCCAGGCTCTGGAATCTCATGCAAATAC CCTGTTCCCCACTGTTCAAGTTTGCTGCGTATGGGGCTTAAACAGTGCCATCCCCCCGCCATCCCATTTC TGAGTCCCCAGGTTTTCTGCATGCCCTCTGGCTTGCTCTGCCCACCAATCCTGGATGGTCAAGGAACTAG CACAATGGTTCTGACCTCTTTTGCTAAGAGCATGCCTGTTTGCTGGGTTGCTCCTGTTAGGCATATGTGT GTGATTGTATGGAACTGTTAGACTTGCTGCAGTGGGTCATTTGTAGGAATTGTTTCTAGCTAGAGGAACT GATTGGCTTCCAATCTAGCATTTGGGTTATGGGAACTTGGGGTGTGTGGTAGGGCTTCATTTCTTTTGTT CTGAGGTGTTTCTCCTCCCTTTAGTCTCTTTCTCCTCATTTGACCATCTTGGGCCTCTTTTCCCATATCA TCTCCCTAAGAAGATGTGCCTGCTCTGTCCACATATAGCATATATCCAAGGCCAGAAGTCAGACCTGCAT ATTGGATTAATACTTTCTCAGTCTTGGGGCCGGGCCTGGGGGCTGGGAAAGGAACTTCTAAAAATAATAG AAAAAAAAATACAAAGGTAAAGTCTTTGAGAGTTTCTGCCTATTTAGATCCTAGAAGGTGGGGTTCATTA GGATTGACTTTGGCAGCCAGGCCAGGACTGAGCCTGCTGAAAACAGAGGCCTTCTAAGCAGCCATGCCAC CACCACTCTAAGTACACAAAGGTCTGGCTCTCGGGCCTCAGTGGCTGCAGAGGTGTGGGAGCACCCAGTC ATCAGGAAGTCTCTTTGGGGTCAGATCCTTTGCCAGTGTGGAGCCCTCAAGTTGAGACTGCATAACTAAA GCAGTCACAGATCTTCTGTGGTTTGGGGTTTTTGTTTTGTTTTGTTTTCTTGTTTTTTACTGCTCTTTTC CAAAAAATGATTTGTAGTTGTGTGTGCAGCACTTTGCCCTAATATGTGTGTGCTCTACAATAAAAACCAA ATCTAATATATTTTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6401541 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401541 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7611 same embryo
	EMAGE:7608 same embryo
	EMAGE:7612 same embryo
	EMAGE:7609 same embryo
	EMAGE:7607 same embryo
	EurExpress:euxassay_004684 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source