EMAGE mouse gene expression spatial database

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EMAGE:7611

Lonp2 lon peptidase 2, peroxisomal ( MGI:1914137)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004680. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004680_01	euxassay_004680_02	euxassay_004680_03	euxassay_004680_04

euxassay_004680_05	euxassay_004680_06	euxassay_004680_07	euxassay_004680_08	euxassay_004680_09

euxassay_004680_10	euxassay_004680_11	euxassay_004680_12	euxassay_004680_13	euxassay_004680_14

euxassay_004680_15	euxassay_004680_16	euxassay_004680_17	euxassay_004680_18	euxassay_004680_19

euxassay_004680_20	euxassay_004680_21	euxassay_004680_22	euxassay_004680_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7611_wholemount_strong.wlz

7611_wholemount_moderate.wlz

7611_wholemount_weak.wlz

7611_wholemount_possible.wlz

7611_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7611_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8683
Entity Detected:	Lonp2, lon peptidase 2, peroxisomal ( MGI:1914137)
Sequence:	sense strand is shown >T8683 GGTGCGTTCGGCGAAGGAAGTGTAGAGTTCGTACTTGGGAGTCCTCCTGACACCTCTGGTGTTGACGACC GTGGCCATGTCCTCCGTGAGCCCCATCCAGATCCCCAGCCGCCTTCCGCTGCTGTTGACCCACGAGAGTG TGTTGCTGCCTGGCTCCACTATGCGCACAAGTGTGGACACGGCCCGCAACTTGCAGCTGGTGCGGAGCCG CTTGCTCAAGGGCACGTCGTTGCAGAGCACCATTCTAGGAGTCATCCCCAACACACCCGACCCGGCCAGC GACACGCAGGACCTGCCACCATTGCACAGAATTGGAACGGCTGCCCTGGCAGTTCAGGTTGTGGGCAGTA ATTGGCCCAAACCACACTACACTTTACTGATCACAGGCCTGTGCCGGTTCCAGATTGTGCAGGTCTTAAA AGAGAAGCCATATCCCGTGGCTGAAGTGGAGCAGCTGGACCGACTTGAGGAATTTCCCAATATCTGCAAA AGCAGGGAGGAGCTGGGAGAGCTGTCAGAGCAATTCTACAGATACGCAGTGCAGTTGGTTGAAATGTTGG ATATGTCCGTCCCTGCAGTTGCTAAATTGAGACGTCTTTTAGATAATCTTCCAAGGGAAGCTCTACCAGA CATTCTAACTTCAATTATTCGAACAAGCAACAAAGAGAAGCTCCAGATCTTAGATGCCGTGAGCCTGGAG GATCGGTTCAAGATGACCATACCTCTGCTTGTTAGACAGATTGAAGGTCTGAAATTACTTCAGAAGACTC GCAAACCTAAACAAGATGACGACAAGAGGGTTATAGCAATACGCCCCATCAGGAGGATTCCACACATCCC AGGAACTTTAGAAGATGAGGAGGAGGAAGAAGATAATGATGACATTGTCATGCTGGAGAAAAAAATAAGA ACATCTAGTATGCCAGAACAGGCTCATAAAGTCTGTGTCAAGGAGATAAAAAGACTCAAAAAAATGCCTC AGTCAATGCCTGAATATGCTCTGACTAGAAATTATTTGGAACTTATGGTGGAGCTTCCTTGGAACAAAAG TACAACTGATCGCCTGGACATTCGGGCAGCCCGGATTCTTCTGGACAATGACCATTATGCCATGGAAAAA CTGAAGAGGAGGGTTTTGGAATACTTGGCTGTGAGACAACTGAAAAATAACTTGAAGGGCCCAATCCTCT GTTTTGTTGGCCCGCCTGGAGTAGGCAAAACAAGTGTGGGGAGATCCGTGGCCAAGACTCTGGGTCGGGA GTTCCACAGGATTGCACTTGGGGGTGTGTGTGACCAGTCTGACATTCGAGGGCACAGGCGGACCTATGTG GGCAGTATGCCTGGTCGTATCATCAATGGCTTGAAGACCGTTGGGGTTAACAATCCAGTGTTCCTATTGG ATGAGGTTGACAAGCTGGGGAAAAGCCTGCAGGGGGATCCTGCAGCTGCGCTGCTTGAGGTGTTGGATCC TGAACAAAACCATAACTTCACAGATCACTATCTAAATGTGGCCTTTGACCTTTCCCAAGTTCTTTTCATA GCAACTGCCAACACCACTGCTACTATTCCACCTGCTTTGCTGGACAGGATGGAGATCATTCAGGTTCCAG GGTACACACAGGAGGAGAAGATAGAGATTGCCCACAGGCACCTGATCCCTAAGCAGCTGGAGCAGCACGG TCTGACTCCTCAGCAGATTCAGATCCCCCAGCACACCACTCTGGCCATCATTACCAGATACACCAGAGAA GCAGGAGTCCGTTCTCTGGACAGAAAGTTTGGGGCTATTTGCCGTGCTGTGGCAGTGAAAGTTGCAGAAG GCCAGCATAAGGAAGCCAAGCTGGACCGTTCGGATGTGGCTGATGGAGAAGGTTGCAAAGAACATGTGCT GGAAGATGCAAAACCTGAATCCATCAGTGACACTGCAGACTTGGCCCTGCCACCTGAAATGCCAATTCTG ATCGACTCCCATGCCCTGAAAGACATCCTGGGGCCACCACTGTATGAGCTGGAGGTATCTGAGCGTTTGA GTCAACCTGGAGTGGCAATAGGGTTAGCATGGACTCCCTTGGGTGGCAAAATCATGTTTGTGGAAGCAAG TAGAATGGACGGTGAAGGCCAACTAACACTGACCGGCCAGCTTGGGGACGTCATGAAGGAGTCTGCCCAT CTCGCCATTAGCTGGCTTCGAAGCAATGCAAAGAAATACCACCTGACTAATGCTTTTGGAAGTTTTGATC TTCTTGACAACACAGACATCCATCTGCACTTCCCAGCTGGAGCTGTCACAAAAGATGGACCATCTGCTGG TGTTACCATAGTAACTTGTCTCGCCTCACTTTTCAGTGGGCGGCTTGTGCGCTCAGATGTAGCCATGACT GGGGAAATTACGCTGAGAGGGCTTGTTCTTCCAGTGGGTGGAATTAAAGACAAAGTCCTAGCAGCACACC GAGCAGGACTGAAGCAGATCATAATTCCGCAGAGGAACGAAAAGGACCTTGAAGAGATCCCAAGCAACGT CAGACAGGATCTAAGTTTTGTTACAGCAAGCTGCCTGGATGAAGTTCTAAATGCAGCTTTTGATGGTGGC TTTCCTGTCAAGACCAGACCTGGTCTCATTGACAGCAAACTATAGGCCCGAACATAAGCTGAATTTAATT ATGAGGCAAAGCTTGTTTGAGGCACCAAATGATCAAGCAAAATATCCCTAATCTGTGGATATAATCAAGA TAGCAATGAGTCTGTTTAAGTAGTTGTTTATTACAGAAATGTTGGCTTAATAAAGTCATTAAACCTAGAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6401542 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401542 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7608 same embryo
	EMAGE:7610 same embryo
	EMAGE:7612 same embryo
	EMAGE:7609 same embryo
	EMAGE:7607 same embryo
	EurExpress:euxassay_004680 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source