EMAGE mouse gene expression spatial database

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EMAGE:7620

Tmem177 transmembrane protein 177 ( MGI:1913593)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004710. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004710_01	euxassay_004710_02	euxassay_004710_03	euxassay_004710_04

euxassay_004710_05	euxassay_004710_06	euxassay_004710_07	euxassay_004710_08	euxassay_004710_09

euxassay_004710_10	euxassay_004710_11	euxassay_004710_12	euxassay_004710_13	euxassay_004710_14

euxassay_004710_15	euxassay_004710_16	euxassay_004710_17	euxassay_004710_18	euxassay_004710_19

euxassay_004710_20	euxassay_004710_21	euxassay_004710_22	euxassay_004710_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7620_wholemount_strong.wlz

7620_wholemount_moderate.wlz

7620_wholemount_weak.wlz

7620_wholemount_possible.wlz

7620_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7620_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8701
Entity Detected:	Tmem177, transmembrane protein 177 ( MGI:1913593)
Sequence:	sense strand is shown >T8701 GGCGCGGGAGCGGCGTGTTCTAAGGTTGCTACCGAGTTGCTCTACACCTGGCTGCCAATGGGAAAGCTGA TTCTGTGAAGCCTGAGGTGTCGGACTCTGGATGGAACCCGTACTCTCAGCCACAATGCAGTGTCACTTCA GTTAGGAACATTGTGGCCCCGGAGGTTGTTTAGCCCACAACCTGACAGAACCATCGGCTGACTTCTCTTC TTCTTGGCCAGTCTATCAGCAGAGGCAGTACTCATGGCTGGTCCTCTGTGGAGGGCTGCAGCCTTTATAC AGAGACACAGAACAAGCCTATTGGTGGGTTCCTGTGCAGGCCTGTTTGGGGTTCAAATCTCATTTCACCT CTTCCCAGATCCCATAGTCCAGTGGCTCTACCAGTACTGGCCTCAGGGCCAACCAGCTCCTCTTTCCCCT CATCTGTGGAGCCTCTTCCAAGAGGTGCTAAAGGACATAGGTGTCCCTTCGGGTCACTGCTACAAGCCTT TTACCGCCTTCACTTTTCAGCCTGTGAGTGCTGGATTCCCACGGTTGCCTGCTGGGGCTGTGGTGGGAAT CCCTGCCATCTTCCTGGGTGGCCCTGTGACCAACATTGAACACTCTGTGATCATACATGGGCAAAGAGTG GATTGGCAAAGCCCAGCAGGCACCAGGCTGAGAGATGCCCTGACCATGTCTCATAATGCCCAGAAGTTTG CCTTGGCCAAGGAGGTGGTATACCTGGAGAGTGGTGTGGCTGCCCTACAGACCCTGCCAGCCCCAGCTTG CCTAGCAGGAACATGGGCAATAAGTGTGGGTGCCAAGCATGCCCTGGGGCTCTACGGAGGCCCCATGAGC TTGCGAACTGCCTTTAACTTGGTGGCAATAGTGGTAGGCTATGTAGCCTATACCTTTTCCAAAGACTCTC TCACTCTTGCCCTGGAAGGCTGGCTGGACCGCCGCACGGCCTCTCTGTCTGCAGCCTATGTCCAGGGTGG AGTGGAATTCTATGAGAAGATTCTGTCAGGAAACTTAGCCCTACGCAGTCTTTTGGGCAGACAAGGAGAA AAGCTGTATACACCAAGTGGAAACATTGTCCCCAGACACTGGTTTCGCATCAACCATTTGCCCTACACCA CCCGCCGGGACTCTCTACAACAGATGTGGAGGGCAACAGTCAGCCCAGGCCGCTTCTGAGAATCCAGGCA ACAGGGCAGTCCTGGCTTGCATAGGCACTGCCTCGTGATGAACTTGAGATGAATCTCTTAGTGTGGTTGC ACCCACAGCCCAGGGTCAGAAGAGTGACCGTCTTTGGAGTTAAACAATTCAACTCTGTCCTCGCCATGAA TAGACTCTGGCTGAGCGTGAGTCCCTATATGAATCTTGAGTCTTGTTTACCTCAACTATGAAGTGGGGAG GACTGATTGTAGGGTTGTATGAGATTGTGAGCCTGAGGGCCTGGTAGAACGCAGCATAAATAAACAGGGC TTTCTTCCCTAGTGGTCTCTGAATTCCAGTGGACTGTGGAAGTCAAACCATGAGTCACCACCAGACATGT TTCTGATATATCCACCCTTTCTTTCTTGCAGTGGAGAACTGCCCTAATCTACCAGATAGCATATAGCTAA TTTATCCCTTGCCTTGATCCTTGGACAAGAGACCCTAGCAGGACAAGGTGAGGAGACTTGTGATCACAGA GCTAGACCTAACTTGGCCATTCACATCATCTTCTCCTTGGAAATTCTCTCTTCCCTGGGCAGTCTTGAAG GATACCAGCCTGGGTCCAGTGTAACTCAGAGGAAAGGCCACACACCATAGGAAGGGTACTCTGGTGGCTG TGGTAGGCTGAAGAATGGCAGGGTGGGATAACCAGTGGCTTCTACTCAGACTTTGTATGTGGTTTAGAGT TGAGTGCACTGCCAGCCACGGGTCCTTTCCCCTGGTCATGGCAGGCTGAGCTCTTGAGGACAAGGGACTC TAGAGCAGAATACAATATGGGACTGATACGTGGATGGTGTTCTAGCTTTCTCTTCTGTAGATGTGATAAA AAAATACTCTGACCAGAAGCAACTCTAGTAGGAGAGGTTTACACTTCTAGATCATATCCTCCAATCCATC TTGGAGGGAAGTCAGAACAGGAACTCAAGTCAGCCCTGGTGTCAGGGACGATGAAAGAATGCTGCCTTCT GGTAACTCTTAACTCCCAGTCTCTGCTCAGCTAGCTTTTTATACAACCCAATACTACCTGCCTATGGATG CTACTATCCATCCATTAATAGTGAAGATAATCCCCCACAGACACACAGAGACCAGTCTTCAGTTGAGGCT TTCCCTTCTCAGGACTGTAGGCTTTGTCAAGTTGACAAATCTAACCAGGACAGATGGATAAAGTAGTTAC TAGATGGATGGCTCTCCATCCAGGCTGACAACACTCTACATCCCTCAGTCACTGCTGTTGCTGTATGCTG CTAGCTAAGGGCCAAAAGTCCACATCAACACCCACATGGAAAAGTATGGACACCGTGGCTCCATCTTCTT GGCCCTTAACACTCTAACACTACACTATCTACTCTTAATCTTGCGAGGAACCAAGATGGTGGCAGCAGGC CCCTCAATTTATTTGCTCAAGTGCTAGGGTTTAAATATGGAAGCTGTGTCCCCATTGTGGCAGTGTTGAA AGTAGGGAAGCCTTTGAGAGGTGGGGCCCAGTGGGAGGTGATGAGGCCCTTTGGGGTATCATCTTCAGGA AGGATTTGTGTTGGCCTTGCACAGTGAGCTCTGGCAGGCACAAGTAACTAAAGCTTTGGTTTACTTTAGT TTTTGAGATGAGGTCTCCCTGTGTTGCCCTTGCCAGCTCAGCCTTCTGAGTAGCTGTTGCTGCAGATGAA CCACCATACCAAGCACAGTTGTTAGAATAAGAGCAAGACCAGTGCAGAGTCCACACTGTGGTATACATCT GGAATTGCAGCATTTTGGGCAGGAGGATTATTAGTTTGAGGCCAGCCTATGCTACACAGTGAAACCCTGT CTTAGAATAATAAACATGCCATGGTGCCTCACACATGTGAACCCCAACACTTGGTAGGTTGAAGTGGTTA GAGGTTAACATGGGTTACGTAGTGGTGAATTCTAGGCAAGACTGACCTAGGCCAAAACCCTATCCAAAGA GCAAGTCCTTCCCCCATGGCTTCCCGATATTGTACCCTCTTCCATGACACCTTCTTCAAGGTGTCACCAG AGATGAGCATGAAGAAGAAGGTGATATTTTCCTGAACCTCCAGAATTATGAGCTAAATAAACCTCTTAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402386 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402386 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7624 same embryo
	EMAGE:7623 same embryo
	EMAGE:7622 same embryo
	EMAGE:7621 same embryo
	EMAGE:7619 same embryo
	EurExpress:euxassay_004710 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source