EMAGE mouse gene expression spatial database

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EMAGE:7626

Prpf38b PRP38 pre-mRNA processing factor 38 (yeast) domain containing B ( MGI:1914171)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004731. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004731_01	euxassay_004731_02	euxassay_004731_03	euxassay_004731_04

euxassay_004731_05	euxassay_004731_06	euxassay_004731_07	euxassay_004731_08	euxassay_004731_09

euxassay_004731_10	euxassay_004731_11	euxassay_004731_12	euxassay_004731_13	euxassay_004731_14

euxassay_004731_15	euxassay_004731_16	euxassay_004731_17	euxassay_004731_18	euxassay_004731_19

euxassay_004731_20	euxassay_004731_21	euxassay_004731_22	euxassay_004731_23	euxassay_004731_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7626_wholemount_strong.wlz

7626_wholemount_moderate.wlz

7626_wholemount_weak.wlz

7626_wholemount_possible.wlz

7626_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7626_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8705
Entity Detected:	Prpf38b, PRP38 pre-mRNA processing factor 38 (yeast) domain containing B ( MGI:1914171)
Sequence:	sense strand is shown >T8705 CGGAAGAGATCGAGCTCCTCTGCTGCCCGTTCTGCTTTGCGTCCGGAGTTCTCCCCGCCTCGTTTTTGGC GTCCGTCTCGGGCCCGGGCTCCCGTGTGTCGGCGTCCCAGTTGCAGTTTCCCGCTCGGCTGGGGCACAAC GCGGCGGGCTCCTTGCCCCGACGGCCTTCCATGGAGTAGGTCCGGGACCGTTGTCCCGAAGAGCGCGATC GAGCTTGGCCTCCCTCCTCCCGCCCGCCCTCCTTCCTTCCTGCCGCGGCACCATGGCCAACAACAGCCCC GCGCTGACCGGCAACTCGCAACCGCAGCACCAGGCGGCCGCGGCCGTGACCCAGCAGCAGCAGCAATGTG GCGGCGGCGGCGGCGCCACCAAGCCGGCGGTGTCGGGCAAGCAGGGCAATGTGCTGCCGCTGTGGGGCAA CGAGAAGACCATGAACCTCAACCCCATGATCCTCACCAACATCCTGTCGTCGCCTTACTTCAAAGTGCAG CTCTACGAGCTCAAGACCTACCACGAGGTGGTGGACGAGATCTACTTTAAGGTCACGCATGTTGAGCCAT GGGAAAAGGGGAGTAGGAAAACTGCTGGCCAGACGGGGATGTGCGGAGGGGTTCGAGGTGTTGGAACAGG AGGCATTGTTTCTACAGCATTTTGCCTTCTATACAAATTATTTACCCTGAAGTTAACTCGAAAGCAAGTG ATGGGTCTCATAACACATACAGACTCTCCATATATCAGAGCCCTTGGATTTATGTATATAAGGTATACCC AACCCCCTACAGACCTGTGGGACTGGTTTGAGTCCTTCCTTGATGATGAGGAGGACCTAGATGTCAAAGC TGGTGGAGGCTGTGTAATGACCATTGGAGAGATGCTTCGTTCTTTTCTCACAAAACTGGAATGGTTTTCT ACTTTGTTTCCAAGAATTCCAGTTCCAGTCCAGAAGAACATTGATCAGCAGATTAAAACTCGACCTAGGA AAATCAAGAAAGACGGAAAGGAAGGCATTGAGGAGATAGACAGACATGTTGAGAGACGGCGATCCAGATC TCCAAGAAGATCACTGAGCCCACGGAGGTCTCCAAGAAGATCAAGAAGCAGAAGTCATCACCGGGAGGGC CATGGATCTTCTAGTTTTGACAGAGAATTAGAGAGAGAGAAAGAACGCCAACGACTAGAGCGGGAAGCCA AAGAGAGAGAGAAAGAAAGGCGAAGATCCCGAAGCATTGATCGGGGCTTAGATCGCAGGCGAAGCCGCAG TCGGGAGAGGCACAGAAGTCGCAGTCGCAGTCGTGATAGGAAAGGGGATAGAAGAGACAGGGATCGGGAA AGAGAGAAAGAAAATGAGAGGGGTCGAAGACGAGACCGAGATTATGACAAGGAGAGAGGCAGTGACCGAG AAAGGGACAGAGAGAGATCAAGAGAGCGCTCCAAGGAGCAGAGAAGTAGGGGTGATGGAGAAGAGAAGAA GCACAAAGAAGACAAGGAGGACAGACGACACAGAGATGACAAAAAAGAGTCCAAGAAAAAACACAGCAGA AGCAGGAGCAGAGAGAGGAAACACAGGAGCAGGAGCAGAAACGCAGGGAAGCGGAGCAGGAGCAGGAGCA AGGACAAGTCAAGCAGACATAAAAACGAAAGCAAGGAGAAGGCAAATAAAAGAAGCAGAAGTGGCAGTCA AGGAAGAACTGGCAGTGTTGAAAAGAGAAAACGAGAACACAGCCCTAGCAGAGAGAAGTCTAGAAAGCGC AGCAGGAGCCAAGACCGTTCCCACAAGAGAGAGCATAATGATAGCAAGGACCAGTCAGACAGACAGGATC ATCAGAGCAGCCAGAGTGGAGAGCCAGAGAGCCAAGAAAAGGAACACAAAAGCAAAGATGAGACTGTGTG AAGATTGTGTAAACTTGGATCACATTGAATCCTATAAATGTTGATTAAATTCTGCTTTTTTCCCCCCAAG TTGAGATTGTGCAGTAGTTATGCACTCTGAGCTCCCCGTAGGCTGTGTTTTCTTTTGTGTAGGGAAGTGC CTTTGTAATCCCATTACGCATTGATGTTTTCACCCTGTTGAGTTGACACATGATGCACAGAATGTTCTTG CATTTTTGTTTGTTTTTAAAATGTACAGTCTGTACATACGTCCTGGAAAATGTTTTAATTCCTTTGGCAT GGTTGCCATGTTGGTTAAATTTGTATAAGGCAATAAACTGCCACTAATTCTATTTTTGTTTTGTAGGTGT GAGATTATGGTTGTGTACTGAAGTTAGCATGGCTGTGCTTTTCATAATAGAATGCTAAAGACTTTGGGAA TGGATCTTGGATGTTTGTTTAGGAGAATTATGTGTTTTTCAGTATACATGGAGGCAACAGTTGGAGGGTT AACATTCTTGTCCACTGTATATTACCTTGGAAGCTCTTGTTAATATGTTGTTATTCTCCACAGTTAATGT TCGAGAGAATTTTTAAGAGGTTTAAATTAGGCTTAGATTTGATCAGAAGTCCTTTTAGCATTCTACCTCA AAGGGACACTGTTAGTATGCCTAAAATTTATTACTTTCCTTTTTACAATGTGCTTCCCCCTCCCTCTTGA ATTCTTTCTCAGACTTTAAAAGTACTATATTAAAGTGTGTAAGCCTGGCGTTGTAGCAGCCCTATACTAA GGAGTGATTGGGAGAGGGCGGGGCGCAGGAGCAGGCGAGGGCGGGGCGCAGGAGCAGAGGAGCAGTCAAG CCTAAGCTTCCAAAGCATTTTATAATGGAGAATACTTAAATTATGAAACAGCTTGATATAGTATCCTTTT TTAAGAATTTAAAACTTTTTTTATTGATAATGAAGATTGCTGCTTGAGTTTTTAAACTTAATCTAGGACA GAAATATTACCAGTAATGCAGTGCTGCGTTATTTAAGACTATCAGCAAATTATTTGATAGATTGTTCTTA TGACTTGTATTCTGATTTCAGAAAACCATCATGAGTGTGGAATAAATACTGGATTAAATCCTTAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402417 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402417 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7625 same embryo
	EMAGE:7629 same embryo
	EMAGE:7630 same embryo
	EMAGE:7627 same embryo
	EMAGE:7628 same embryo
	EurExpress:euxassay_004731 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source