EMAGE mouse gene expression spatial database

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EMAGE:7627

Pomt2 protein-O-mannosyltransferase 2 ( MGI:2444430)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004730. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004730_01	euxassay_004730_02	euxassay_004730_03	euxassay_004730_04

euxassay_004730_05	euxassay_004730_06	euxassay_004730_07	euxassay_004730_08	euxassay_004730_09

euxassay_004730_10	euxassay_004730_11	euxassay_004730_12	euxassay_004730_13	euxassay_004730_14

euxassay_004730_15	euxassay_004730_16	euxassay_004730_17	euxassay_004730_18	euxassay_004730_19

euxassay_004730_20	euxassay_004730_21	euxassay_004730_22	euxassay_004730_23	euxassay_004730_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7627_wholemount_strong.wlz

7627_wholemount_moderate.wlz

7627_wholemount_weak.wlz

7627_wholemount_possible.wlz

7627_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7627_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 20 21 22 weak expression: see section 18 19

foot

moderate

regional

moderate expression: see section 01 weak expression: see section 02 18 19 20

femur

weak

regional

weak expression: see section 03 16

posterior naris

moderate

regional

moderate expression: see section 10 11 13 14

viscerocranium

moderate

regional

Expression in the turbinate bone.

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 14 15 16 17 18 21 weak expression: see section 12 13 19 20

maxilla

moderate

regional

moderate expression: see section 05 07 08 09 17

axial skeleton

weak

regional

weak expression: see section 09 10 11 12 13 16 17

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8709
Entity Detected:	Pomt2, protein-O-mannosyltransferase 2 ( MGI:2444430)
Sequence:	sense strand is shown >T8709 AGCGGACAAGACCCGGCGTGCCTCCGGCCCGGAGGGTGATCCTTCGGAAGGGGAGGATGCCGCCGGCCAT AGGCGGTGGCCTAGCGGGCTCCGAGCTGCGTCCCCGTAGGGGCCGCTGTGTGCCGCAGGCTGCCAGAGCC GTGAGCCGAGACGTGGTCCCCCAGGCTGCAGCACGGAAACTTAAACGGCCTGCTTGGAGCTCGCGGCGCT TCCAGGCGGCAGGGTGGTGGGCCACGTTGGCTGTGGTGACGCTGCTGTCCTTTGCCACCCGCTTTCACCG GCTGGACCAGCCTGCTCACATCTGTTGGGATGAGACTCACTTTGGAAAAATGGGAAGTTACTATATTAAC CGCACCTTTTTCTTTGATGTGCATCCACCACTGGGAAAGATGCTGATCGGTCTTGCTGGCTACCTGAGCG GGTATGATGGTACCTTTCTGTTCCAGAAGCCTGGGGACAGATACGAGCACCACAGCTACATGGGAATGAG AGGATTCTGTGCTTTCCTGGGTTCCTGGCTGATCCCCTTTGCCTACCTCACCGTACTGGATCTGTCCAAG TCCTTCCCAGCAGCACTGCTCACCGCTGCCCTTCTCACCTTTGACACAGGATGCCTCACTCTATCCCAGT ACATCCTCCTTGACCCCATCCTGATGTTCTTCATCATGGCGGCCATGCTGAGCATGGTCAAGTACAACTC CTGTGCTAACAGGCCTTTCTCTGCCCCCTGGTGGTTCTGGCTCAGCCTGACTGGCATTAGTCTCGCTGGT GCTTTAGGGGTCAAATTTGTTGGCCTCTTTATCATCGTGCAAGTGGGGTTGAACACTATTTCAGACCTCT GGCATCTGTTTGGAGACCTCAGCCTTTCACTGGTGACTGTGGGGAAGCACCTAACTGCTCGCATCCTGTG CCTCATAGTGCTGCCTTTGGTGCTCTATGTGACCATTTTTGCTGTTCATGTCATGGTGCTGAATAAAAGT GGTCCTGGTGATGGCTTCTTCAGTTCTGCCTTTCAAGCCCGGCTTTCAGGGAACAGCCTTCACAATGCTT CTATCCCTGAACACCTGGCGTATGGCTCTGTAATCACTGTGAAGAATCTCCGGATGGCCATTGGCTACCT GCACTCTCATAGGCACCTCTACCCTGAGGGCATTGGTGCTCGCCAGCAGCAGGTTACCACCTATTTGCAT AAGGACTACAACAACTTGTGGATTATCAAGAAGTATAATGCCAACACAGATCCCCTAGACCCTTCATTCC CAGTGGAGTTTGTCAGACATGGAGACATCATACGACTAGAACACAAAGAAACTACTCGGAACCTGCACAG TCACTATCATGAGGCTCCCCTGACCCGGAAGCACTATCAGGTCACTGGCTATGGCATAAATGGGACAGGG GACTCAAATGACTTCTGGCGGATTGAAGTTGTAAATAGAAAATTTGGGAACCGGATCAAGGTACTGAGAA GTCGAATTCGCCTCATCCATTTGGTCACAGGTTGTGTCCTGGGATCGTCAGGAAAGATTCTGCCTAAGTG GGGCTGGGAGCAGTTGGAAGTTACCTGTACTCCGTACCTAAAAGAAACCACTAACTCCATTTGGAACATA GAAGAGCATATCAATCCCAAATTGCCAAACATCAGCCTGGATGTGCTGCAGCCCAGTTTTCCTGAGATCT TGCTGGAGTCTCACATGGTCATGATCCGGGGAAATAATGGCCTCAAACCCAAGGACAATGAGTTCACATC CAAGCCCTGGCACTGGCCTATCAATTATCAGGGCCTGCGCTTCTCAGGGGCCAATGACACGGACTTCCGA GTCTATCTGCTCGGCAACCCTGTGAGTATCCCAGCTCTGAGTGCTTAGGTCCCAGCCTGCAGAAGCTGGT GTCCTTTCATCCCCAGCTCTCTTTCCATACAGGTGGTCTGGTGGCTGAATCTGGTGAGCATTGTCCTCTA CCTCCTCTCGGGGAGCACCATTGCTGTGGCTATGCAGAGAGGGATACAACTGCCTGCAGAGTTGCAAGGG CTGACCAAGGTGCTGCTGCGAGGAGGTGGCCAGCTGCTCCTGGGATGGATGCTCCATTACTTCCCTTTCT TCTTGATGGGCCGGATCCTCTACTTCCACCACTACTTCCCAGCCATGCTCTTTTCCAGCATGTTGACAGG TATCCTATGGGATACTCTCCTGCGGCTCTGTGCCTGGGGCTTGGCCCCTTCTCCCCTGGGTAGGAGGATA CATGCGGTGGGAATCCTGAGCCTGCTTCTGACAACTGCCTACAGCTTCTACCTCTTCCACCCTCTGGCTT ATGGGATGGTTGGTCCCCTAGCCCAGGAACCTGAGAGTCCAATGGCAGGACTGAGGTGGCTGGAATCATG GGACTTTTGAGGCCACTGCCAGGACCCCAGCCTGGATCCGGGTTTTATCCAGGGACACCCAGCTATGAAG CCAGATTCTGGACCCTTCCAGCTCCAAGGGAGCTGCCTGAGGAACCCAAAACATTCTGCTGCCCCCAGGA CCAAACTCTGGGAAATAGTCCTGGACTTAACTAAATACTAAAAAGAGCCACAGCCCTGTCTACAGCACCT GCACGGGGCACGTGACCTACAGCACTGCTCGGTGCCAGTTCCTGTGAAGCACAAGGAAAGCAGGCATGAG TGTCAGAGGGTACATCTGCCCAGTCCCTTTGGAGTGTGTAGACCATGTAACTGTGGCAGCCAAGTGTCAT AAATTCCAAGAAATGGTCCAAGGTTAACAGGATATTCTCTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402328 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402328 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7625 same embryo
	EMAGE:7629 same embryo
	EMAGE:7630 same embryo
	EMAGE:7626 same embryo
	EMAGE:7628 same embryo
	EurExpress:euxassay_004730 same experiment
	MGI:4827331 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source