EMAGE mouse gene expression spatial database

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EMAGE:7775

Cdc23 CDC23 (cell division cycle 23, yeast, homolog) ( MGI:1098815)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007491. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007491_01	euxassay_007491_02	euxassay_007491_03	euxassay_007491_04

euxassay_007491_05	euxassay_007491_06	euxassay_007491_07	euxassay_007491_08	euxassay_007491_09

euxassay_007491_10	euxassay_007491_11	euxassay_007491_12	euxassay_007491_13	euxassay_007491_14

euxassay_007491_15	euxassay_007491_16	euxassay_007491_17	euxassay_007491_18	euxassay_007491_19

euxassay_007491_20	euxassay_007491_21	euxassay_007491_22	euxassay_007491_23	euxassay_007491_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7775_wholemount_strong.wlz

7775_wholemount_moderate.wlz

7775_wholemount_weak.wlz

7775_wholemount_possible.wlz

7775_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7775_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9039
Entity Detected:	Cdc23, CDC23 (cell division cycle 23, yeast, homolog) ( MGI:1098815)
Sequence:	sense strand is shown >T9039 GCTGCGAATTCTTCGGTAGTCTCGGTGGCAGCGGCCGCCACGGCGGTGCCGGGCGTATCCACTGTCGCGG ATTTTTCAGACTTGCAGGAAATTAAAAAGCAGCTGCTGCTCATCGCGGGCCTCACTCGGGAGCGGGGCCT GCTGCATAGCAGCAAAATGGTCTGCGGAGTTGGCCTTCTCTCTGCCCGCCTTGCCTTTGTCGGAGCTGCA GCCGCCGCCGCCTCTGACAGAGGAGGACGCGCAGGATGTGGATGCTTACACCCTGGCCAAGGCCTACTTT GATGTTAAAGAGTATGACCGGGCGGCACATTTCCTTCACGGCTGCAATAGCAAGAAAGCCTATTTCCTGT ACATGTATTCCAGATACCTGTCTGGAGAGAAGAAGAAGGATGACGAAACAGTTGATAGCCTAGGACCCCT AGAGAAGGGACAAGTCAAAAACGAGGCTCTCAGAGAACTGAGAGTGGAACTCAGCCGGAAGCACCAGGCT CGGGGACTTGATGGATTCGGCCTTTACCTGTATGGGGTGGTGCTTCGGAAACTGGACTTAGTGAAAGAGG CCATTGATGTGTTTGTGGAGGCTACTCATGTTTTGCCTTTGCACTGGGGAGCCTGGTTAGAGCTGTGTAA CCTGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCCTGACACCTGGATGAAAGAGTTCTTCTTG GCTCACATATACACAGAACTGCAGCTGATAGAGGAGGCCCTGCAGAAGTATCAGCATCTCATTGATGTGG GCTTTTCCAAGAGCTCGTACATTGTTTCCCAAATTGCAGTTGCCTATCACAATATCAGAGATATCGACAA AGCCCTTTCTATTTTCAATGAGCTGAGGAAACAAGATCCTTACAGGATTGAAAATATGGACACATTCTCC AACCTTCTCTATGTCAGGAGCATGAAGTCTGAATTGAGTTACCTGGCGCATAACCTCTGTGAAATTGATA AATACCGAGTAGAAACATGCTGTGTAATTGGCAATTATTACAGCTTACGCTCCCAGCATGAGAAAGCAGC TTTGTATTTCCAGAGAGCCCTAAAGTTGAATCCTAGGTACCTTGGGGCCTGGACACTGATGGGGCACGAG TACATGGAGATGAAGAACACGTCTGCAGCTATTCAGGCTTACCGACATGCCATTGAGGTCAACAAGAGAG ACTACAGAGCTTGGTATGGCCTTGGGCAGACTTACGAAATCCTTAAAATGCCATTTTATTGCCTTTATTA TTATAGACGGGCCCACCAGCTTCGTCCCAATGATTCTCGCATGCTGGTTGCCTTAGGAGAATGTTATGAG AAACTCAATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCATATGCCGTGGGAGACGTGGAGAAAA TGGCTCTTGTGAAGCTGGCCAAGCTTCATGAACAGTTGACTGAGTCAGAGCAGGCTGCCCAGTGTTACAT CAAATACATCCAAGATATCTATTCCTGTGGGGAGACCGTGGAGCACTTGGAGGAGAGCACAGCCTTCCGA TACCTGGCCCAGTACTATTTTAAGTGCAAGCTGTGGGATGAAGCTTCAACTTGTGCCCAAAAATGTTGTG CATTCAATGATACCCGGGAAGAAGGTAAGGCCTTGCTCCGCCAGATCCTACAGCTGAGGAACCAAGGGGA GACACCCACCTCTGACACGCCGGGTACCTTTTTCCTCCCTGCCTCACTGTCTGCAAACAACACTCCTACA CGCAGAGTTTCTCCACTCAACCTGTCTTCAGTCACACCATAGTCGACTACCCTCACGTCAGCACATTGCT AGACCAATATCAAACCTTACCTGCAGTAAAGAGCAGTCACGTCTGTTTTCCCAAGGACCTAGACAGAAAC AGCCTGAGGGACAGCCTATGGAACCACCTTCAGAGGCAGGCAGAAATATGGTGGGGGAAAGATGGTCTTC TGCAGCCAGCTAGCAGTGCTCCCTTCTATTCAGAGTGGCAGGTCTTGGCCTTTCTAGCCACTCCCCCAAC CCCCCAAGGAATCCCTTTTACAACACTTAACCAGCAGTGATGCCACAGAAGCAAGGTTTCGGCGCTGCTG AGAGGGGTAGACAAGAACGGAGCGAAGCTCACCATGCTTGGTGCCTGTCCTCTGCATGCAGGGAGCTCAG CTGTGACTTTCTTTTTAAAGACAGGGTTTCTGTGTATCCTAGCCTGTGGCCTTTCTGCCTCAGCCTCCCT AATGATAGCATTACAGGCGAATGCTGAGTGAGGCCTAACTAGAACGACCTTTATGTTACTGAGTGTACTG TTCGGATAAGAGATTAGGTTTAGAGTGGAGCTATCCATATGATCTTGTGTAGTTTTCCAAAGCAGCCTTC TAAAATCTTCCAAGTGAGTCAGTGAGCATGGACTGTGGCTCACATGAGGGCCAGATGGCCGCTGTGGCCA TGCTAACTATATATATATGTGATGAAGCAACACAAGAAGAGAACTTCGTGGACATTATGGAAGCTTTTTT ACTTCGGATTTTAAAGTCTCCAATTTGGTTTTCAACCTTATCTACAAGGTTGAAGAATTCACTGTGTTTT TCTAGGTTTCAGAGCCACTTGATGTCTGCTACAATGAGCTTGTGCAGTAGGTGCTATAGATCATTCTGTG GTTAGGAGCTAGCCTTCCTTCTCTTGTACTCAGAACATTTTACTCCCCATTTAATGGAAGGAACACTAAC TGGGTGCCATGTGGTGGTGCATGTCTTTAATCCCATTTGGAGACAGGCAGGTGGGTGTTTTGACATAGCA ACTTCCTGGCCAGACAAAGCTACCCAAGCCTCTTGGGTATCATTTAAAATTGTACTCATTTCCTAGTGTG CATTAGGCTGGTTCCCACTGCTGTTCCTGTCCAAGGGTCTAACAGGCCAGATGGTATTTATATCAATTTT CTGTCCCCCTTTTTATATTTTTCTAAATTTGGAATCCAAATAAAAGCATAAACAATGAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:6813730 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6813730 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7778 same embryo
	EMAGE:7776 same embryo
	EMAGE:7780 same embryo
	EMAGE:7777 same embryo
	EMAGE:7779 same embryo
	EurExpress:euxassay_007491 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source