EMAGE mouse gene expression spatial database

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EMAGE:7778

Tnks tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase ( MGI:1341087)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007391. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007391_01	euxassay_007391_02	euxassay_007391_03	euxassay_007391_04

euxassay_007391_05	euxassay_007391_06	euxassay_007391_07	euxassay_007391_08	euxassay_007391_09

euxassay_007391_10	euxassay_007391_11	euxassay_007391_12	euxassay_007391_13	euxassay_007391_14

euxassay_007391_15	euxassay_007391_16	euxassay_007391_17	euxassay_007391_18	euxassay_007391_19

euxassay_007391_20	euxassay_007391_21	euxassay_007391_22	euxassay_007391_23	euxassay_007391_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7778_wholemount_strong.wlz

7778_wholemount_moderate.wlz

7778_wholemount_weak.wlz

7778_wholemount_possible.wlz

7778_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7778_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9031
Entity Detected:	Tnks, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase ( MGI:1341087)
Sequence:	sense strand is shown >T9031 GTATTCAAGTTGGCAAAAAGGTGGTTGCTTAGGATTTCCTTGTACAGCAGCATAGACTGTAGAAGAGCCC TCTGGACTAGACAAGCCTGGCATTCGGCTGACAGCTGTTTACCTTCTTGTATGTGCATTATGTAGAACTC CTGGCAGGTGTGGAACCAACTCCTGCTTCCCAACAGAGCTGCAGAGAGAAGGCAGGTGGCTCCAAGCTCT TCTTCAAGGCCTTTCCCAGAGACCCCACAGTGCCACTTTACTGGTTTGGCCAGCTTGGATACTTAACTCA GGCATAAACTATAATTACTCTGGGCACGTCTGCCTGTACCTTTGCTTCTCCCCAGATTGCAAAGCTAGTC TTGGAAGTGCTCCTGTGCTTGCCCCATTCACCTTTTTCCTTTTCTTCATCACGAGTCTTATATAGGAATC CAGGTGTCCCTTATTTTCCTATCAGAATGGATCAGGGTTTAGTCCTCATGTAGTTTCTGCTCAGAAGTCT GCCAGTTTGCTAACAGATGGCAGCATGGAACCCAGTGTTTCTATGCAAAAACACAATGGCAGTACAGTCT GGGTTCTAACCCTGCTTTCCCTCCCTCACAGAGCTCTGAGCAGCCTAAGTAGAGTAACAGCACTAACATC GCCTCCTGAAGGCAGCAGGCTCCTCTGAGCTCCACACTTCTGACTGCACTCATTTCTCTTGGCTGTTGGA ATAGTGAAGGGCATTTGGAATGTATAGAGAAATACTTGAATATGGCAAGCAAATAAGCATACATTAACAT CTTATTATCCTAGATATTATTACAAACAGATATTAACATTTGTCTAGTGCAGAAAACAGCATTAGAATGT AGTAAATGTCCCAATTTTCTTCTACTGCAATATATTCCCAGTTGCATGAAGGGTGCTTAGGCATTGTTCC CTCTCCCCTTTTAAACAGTCGTCATCTGAATTCCTGTAAATGGCCTTGTAAATCAATGTCCGTATTTGTA AGCATATCTGGCTGTCTGTCCCTTCACCCATGCACCCAGACCTCACATGTGTGCCAGCCTTCATCCTGTG TTTCTGTTGGGCAGATCATCTTGCACAGAGTTACAGACTATAGAATCACTCAGCAAATATTCAGAAATGA TGTAAGAATTTTCATGGATCAGAAAGTTGAAGGCTGGCCTCTGATGTTAAGGCAGTGACACAGAAATGAG GGTGATTGTGACAGTAATATTCAAACAACCGCTGACTACATCCAGTGCATGAAAAAGATCACTTAAAAAT ACCGCCCTAAAAAACTGAAGCTGTTTCAGCCAACTCAAGCAATCTGCAGTTAATAGACAACTATGGTTTT CCTGTTTGAAAAAGCCTTATCTATGGCAGAAACTTTTTAACCTTTTCTAATCTAGCAGTGAAATATGAAT CCACTTCTTAATATTTGAAATGTGTCAGTGAGTGGGCGTGTCTGTTCATTTCATACCTGGTAAGTGTGTT TTATGGGGTATATTTTATAAGTAATATTGTCACATTTAGTGGAGTTGATGTAGCCAGTCAGTTCCAATTT CTCTCATGCAAAGTTCTTCAGCCTCCTGGTCACCTGGTTTGGAGTAACACTGGTGGGATTGCCGAACTTG TGTGGGGGATTCATAGTATGTAATACTGTGTAGCATTTGAGTGGTGTGGAAGTTGTTTTTAAATCATTTT AAAGTATATACTATGACAGATGAAACTTAAAACTTGTCGGAAGCTGAAGATTTTATTTCTGTGAGATTCA GTGTGGTCAGGTTGTGTGGCCAATTTTATACTTCTACCAGAATTCTGCAAAATTGAGGTGACGCTCACCC CATAAAGACACTCACCCTATAGAGGTAGTTGTCACACACACACACACACACACACACACACTGTTCACTT CCTAGCTCCTTTCCCCTCCACCTTTCTTTCAGAGTTTGTTCTCATTTTTCAGAATTCTTCTGCTATCCTT CTCAAGTGGCCCTGAATGCCCTTTGTTAGCTCAATTTGGTGATGAGTTATCCCTGAACCTCTCCCAGTGT TCAGGAAGAAAAACTAGAAAACGCCACAGTAAAAGGAAGGAAGGAACTGAGGTTTTTAAAGCTTGTAGCA TAGACCGAGAAGGTGACCATGCGGTATAGTAAATGTATTCACTACACAGAACACTGAGACTCGTCATGTG TGTGTAATGCCTGCTTCTGCTCCCTCCCAGTGTTTTAAACTCATCTTATCTTTGTGGTAGTAATATTTGT CCTTAATAACCTACAAAACTAAAAAGGTACTTTGATTGACAAGGTCCCTCAAGAACTTACACAGCCAGCT TTGAGGAAATCTAGACTGTTCTGACAACAGCATCTGGAGTTGTAATTGTTGTAATTTTCTTCCTGTACTG TCTCAGTCTGTGTAAATAACCAGTGTAGTGGCCCTTTGATTTGTTGTTGGTGTTCTTTTTGTTTCCGGTC TCTGTGTGACCCACCAGTGGCAGGGGTTCCAGCTCCACCTTTTTTTTTTTTGCTTGCAGTTGTCCATCTG TAGGCTTGTCAGACAAAGTACAAGATGCCCAGTTAGAGTTGAATTTCAGGCACTTTTCTAGCTCAAGTAT ATTTCATATTTGACATATCTGTGCAATTTTTAAAACGTTCATTAACTTATCTGAAATTCAGATTTTGACT GAATATCCTAGATTTTTGTTTTCTATGTAGTTTTTTAAACCTGATAGTCCTAGCAATTGATCACAAAAGG AGGCTCACAGCATGTAAATTCTCTGGTCTTTATAGCGTTCACCTGGAATGGACGACCCTTTTTTTTTTCC TTTGCCTTCTACTTATTTCTAAAAGAGGGCATCAAGGAACTTAACCAGCCTACATGGTGGGGTTCTATTT AAAACATCTCTGTGATTATATTTAACCTGTAATTGTGCTTTGGCTTAATGTCTACAGTACTTGTAACGGA TTAATATTCAATAAAAACATTTTAAAGTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6813322 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6813322 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7776 same embryo
	EMAGE:7780 same embryo
	EMAGE:7775 same embryo
	EMAGE:7777 same embryo
	EMAGE:7779 same embryo
	EurExpress:euxassay_007391 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source