EMAGE mouse gene expression spatial database

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EMAGE:7803

Chdh choline dehydrogenase ( MGI:1860776)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005323. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005323_01	euxassay_005323_02	euxassay_005323_03	euxassay_005323_04

euxassay_005323_05	euxassay_005323_06	euxassay_005323_07	euxassay_005323_08	euxassay_005323_09

euxassay_005323_10	euxassay_005323_11	euxassay_005323_12	euxassay_005323_13	euxassay_005323_14

euxassay_005323_15	euxassay_005323_16	euxassay_005323_17	euxassay_005323_18	euxassay_005323_19

euxassay_005323_20	euxassay_005323_21	euxassay_005323_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7803_wholemount_strong.wlz

7803_wholemount_moderate.wlz

7803_wholemount_weak.wlz

7803_wholemount_possible.wlz

7803_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7803_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15

pons mantle layer

moderate

regional

moderate expression: see section 07 16

facial vii ganglion

moderate

regional

moderate expression: see section 03

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 08 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6912
Entity Detected:	Chdh, choline dehydrogenase ( MGI:1860776)
Sequence:	sense strand is shown >T6912 ATTTCCCATCAGTTTCCATTCCAGGCGTACCGGAAAAGACTCTTCCAACTCCTGTCGGTGGATAGAGGAA CAGGGCTGGAAGCATGTGGCAGGCCCTTAGAGGCTGGAGGAAAGGATGTCAGAGCCCACGTGGAGCCCTG GCATGGGCTGTGCGGGGCCAGCCATGCCCCCCTTGCAGCCGCGCTGTTGCCAGTGTGGGCAAGGATGAGT ACACCTTTGTGGTGGTGGGCGCAGGCTCTGCTGGCTGTGTGCTGGCTAGTCGGCTCACCGAGGACCCGAA TCATCGGGTGCTGCTGCTAGAGGCAGGACCTAAGGACTTGTTGATGGGGAGCAAGCGGCTTCAGTGGAAG ATCCATATGCCGGCTGCCCTTGTGTCCAACCTGTGTGATGACAAATACAACTGGTACTATCACACTGAGC CCCAGCCCGGCATGGACAGTCGCGTGCTGTACTGGCCACGAGGCCGGGTCTGGGGCGGCTCCTCATCCCT CAATGCCATGGTCTACATCCGAGGACACGCCGAGGACTATAACCGCTGGCACCGTGAAGGTGCTGAGGGT TGGGACTATGCACACTGCCTGCCCTACTTCCGCAAGGCGCAGAGACATGAGCTGGGTGCCAATATGTACC GTGGTGGGGATGGCCCACTGCATGTGTCTCGGGGCAAGACCAACCACCCGCTTCACCAGGCCTTCCTGCA GGCAGCACGTCAGGCTGGCTACCCCTTCACTGAAGACATGAATGGCTTCCAACAGGAGGGCTTCGGCTGG ATGGACATGACCGTCCACCAAGGTAAGCGCTGGAGCACAGCCTGCGCCTACTTGCACCCGGTGCTGAGCC GCCCCAACCTCAGGGCCGAGGTCCAGACACTTGTAAGCAGAGTGCTGTTTGAGGGCACCCGTGCAGTAGG TGTGGAGTACATCAAGGACGGCCAGAGACACAAGGCTTACGTCAGCAGGGAGGTGATCCTGAGCGGGGGA GCCATCAACTCTCCACAGCTGCTCATGCTCTCTGGCGTCGGCAATGCAGATGACCTCAGGAAACTGGATA TCCCTGTGGTGTGCCACCTGCCTGGAGTTGGGCAGAACCTGCAGGACCACCTGGAGGTCTACGTTCAGCA AGCGTGCACACAGCCCATCACCCTCCACTCTGCCCAGAAGCCTCTGCGGAAGGTCTGCATCGGCCTGGAG TGGCTCTGGAGCTACACAGGGGACGGAGCCACAGCCCATCTTGAGACCGGAGGGTTCATCCGCAGCCGAC CTGGGGTCCCTCATCCGGACATCCAGTTCCACTTCCTGCCATCGCAAGTGATTGACCATGGGCGGAAACC CACCCAGCAGGAAGCTTACCAGGTGCACGTGGGAACCATGAGGGCCACAAGTGTGGGCTGGCTGAAACTG AGAAGTGCCAACCCCCGGGACCACCCTGTGATCCATCCCAACTACTTGTCAACAGAAACCGATGTCGAGG ACTTCCGTCAGTGTGTGAGGCTGTCACGAGAAATTTTTGCCCAGGAAGCCTTGGCTCCATTTCGGGGAAA AGAGCTGCAGCCCGGAAGCCACGTCCAGTCAGACAAAGAGATAGATGCCTTTGTGCGGGCAAAAGCAGAC AGTGCTTACCACCCCTCGTGCACCTGTAAGATGGGCCGGTCCTCTGACCCCACTGCCGTGGTTGACGCGC AAACCAAGGTCATCGGCGTAGAAAACCTCAGGGTCGTTGATGCCTCCATCATGCCCAGCGTGGTCAGCGG CAACCTGAACGCTCCCACGGTAATGATTGCAGAGAAAGCAGCCGACATTATTAAGGGACACCCTGCACTT GAGGACAAGAATGTTCCTGTCTACAAGCCCCAGACTCTGGACACCCAGCGTTAAGACAGACAGCCATTGC CTGAGGACGACAGAGGAACTCAGGCCAAGCCAAGAGATCCAGCCAGTACAGCCCTGCCCTAGATATTTCT GAACCGGTATATAGACACTCGGGACCCAGATACCCCGACTCTTGGCTCAGACTTTCATGGTTTCTGAGGA AATGAGGTCATGGTAGGCAGTGAGGCAAGTCCCTTTTCCCAGTGTCTCTCTGATGCCCCTCCACAAAAAG CCTAGCAAGCAAGCTGGGGCCCTCCCCCTGGCTGCCTGCGGGTAGCAGGTGGGGTAGCTAACATGGTTTT CTTGTCTCCAGTTCAGAGCTCTGCTTCCAGGAAGGACTGGGATGATCCTTTCCCTGGTCTCCCACCTGAG GCCATTCCTGCAAAGTCAGGGTGGTTGAACTGGACTGGGCTCTCCAAGGAAGCTTTTGCTGAAGCCAAGA GGCAGGTAGCAGCTCAGCTGGGCTGGTTACCTGAGCTTATGTCCCCAATTAGAGGGACAGGCAGCAGCTA GAGGACGCCTCATCCCCCTCTGCACTTCTCTCCTGCCCTGATTTAAATCTGAAGAGCGAGGCACAGGGAC GAGGGCTCAGGTGACTGCACAACTCAACGCCTACCACTCTGGCTCTCATGAAACCGTCCAGGTGAGAGGC TTGCAGGACGTTGTTCAAGAAAAGGCCATTTTGGCCTCACTGCCAAAGGTTTTATTGTTTAACCCTTACC ACACAAAAGTGCAGGAGTATAGAGCCAGTCCCACATGCCAGTCACTACCTCATGCCAAGGACATGAGCCC ACGAGGTCCTTTCATGGTGGCCCCAGAAATGGCCCAGGCACCAGAGCTACTTCTGCAGACAGGGCTGGCC GTTTCTTATTCTCTAGATGTGAGAACAGAGAGCATGGCACCAAGGCTGATGGAGAACAGCAAAGGACTTT GGAGTGAAAGATAAACCAGCTAGAATTCGAGTCTTGGGTCAGGGGAGCAGATACCAGCAGCATTTACAAG AGCTCTTTGATCTGGAAATTCCTCATAAATTTGGATGTAACCGGTAACTTAATTTTTGAGCCAAGCTGCC TCATTCCAACTGTTCTTATGAGAGAATGGGGGTCAGACGCATTGTAGGTTAGTTTTCCTGAAGATGTCCA AAGCCATCATCTGTAGTCGGGAAGAAAGGGATCGCTGTATAGAAATCACATGGCTGAGTGAAGAAGAGAG AGGAAATAGCAGGATTCCAGGGTCCCCTAGGGTTGACTGGCAGGAGCTGCCTTCTGGACTTCTGAGTCTG AGAAGACCCTTCAAAGGGTTCAAAAAACAAATTATAAACCAACACTTTTACTTTGAAAACAAGGTTGCCC TTATTTCATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3979709 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3979709 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7805 same embryo
	EMAGE:7806 same embryo
	EMAGE:7804 same embryo
	EurExpress:euxassay_005323 same experiment
	MGI:4823841 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source