EMAGE mouse gene expression spatial database

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EMAGE:7805

0610012G03Rik RIKEN cDNA 0610012G03 gene ( MGI:1913301)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005319. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005319_01	euxassay_005319_02	euxassay_005319_03	euxassay_005319_04

euxassay_005319_05	euxassay_005319_06	euxassay_005319_07	euxassay_005319_08	euxassay_005319_09

euxassay_005319_10	euxassay_005319_11	euxassay_005319_12	euxassay_005319_13	euxassay_005319_14

euxassay_005319_15	euxassay_005319_16	euxassay_005319_17	euxassay_005319_18	euxassay_005319_19

euxassay_005319_20	euxassay_005319_21	euxassay_005319_22	euxassay_005319_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7805_wholemount_strong.wlz

7805_wholemount_moderate.wlz

7805_wholemount_weak.wlz

7805_wholemount_possible.wlz

7805_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7805_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 15 16

pons mantle layer

weak

regional

weak expression: see section 06 07 08 17

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 18

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

abducent vi nerve

moderate

regional

moderate expression: see section 08 weak expression: see section 16

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2948
Entity Detected:	0610012G03Rik, RIKEN cDNA 0610012G03 gene ( MGI:1913301)
Sequence:	sense strand is shown >T2948 TGGCCTCGAGCCAGATTCGGTACGAGGTGGAGCGGCTGTCCGAGTCGCGGCCCATCCGGCGGGCGGCGCA GCTCACAGCCTTCGCGCTGCTACAGCTACAGCTGCGCGGCCACGATGCAGCCCGCCGCCTGCGCGCCCTC GCGGATCAGCCGCCCGGTGCCCTGCGTCGCCGCGTCGTGAGGTTCAAGGACACTTTCACCGAGGAACTCC GCCGTGGCGTCCGGGACCGTCCAGGGCAGCAGAAGGGTCCGGGCGCCAAAGCGTAAGCACGGACTGGACC CTCCCGGGCCGAGGTCACGTACCCGTCCCTTCAGTGATCTACACCGACCTCAGCAGTCACGAGTCATCTC CAGGACGCTACGCTGCTTCGAATCCTAACTCGGATTGTCCTACAGATGCCTAAAGTCAAATGGACATGTG CCCGGTAGTCCTGCAGACGTAAAGACCTCGAGGCCCACCAGTGATAGAGAATCTAGACCATAACAGCACG ACTGAGAGTCATGGACAATCAGAAAGCAAGCCTGCCGTCTGCAAGCAGGACTGAAGCTGAATTCCTGCTG CT
Notes:	The probe template was PCR amplified from IMAGE:1532094 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1532094 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7803 same embryo
	EMAGE:7806 same embryo
	EMAGE:7804 same embryo
	EurExpress:euxassay_005319 same experiment
	MGI:4822583 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source