EMAGE mouse gene expression spatial database

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EMAGE:7812

Cinp cyclin-dependent kinase 2 interacting protein ( MGI:1914486)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005320. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005320_01	euxassay_005320_02	euxassay_005320_03	euxassay_005320_04

euxassay_005320_05	euxassay_005320_06	euxassay_005320_07	euxassay_005320_08	euxassay_005320_09

euxassay_005320_10	euxassay_005320_11	euxassay_005320_12	euxassay_005320_13	euxassay_005320_14

euxassay_005320_15	euxassay_005320_16	euxassay_005320_17	euxassay_005320_18	euxassay_005320_19

euxassay_005320_20	euxassay_005320_21	euxassay_005320_22	euxassay_005320_23	euxassay_005320_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7812_wholemount_strong.wlz

7812_wholemount_moderate.wlz

7812_wholemount_weak.wlz

7812_wholemount_possible.wlz

7812_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7812_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 04 05 06 22 23

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 16 17 18 19 20

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 10 17 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 07 08 09 17 18 19 20 21 22 23

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 12 13 17 18 19

lower jaw incisor

weak

regional

weak expression: see section 13 14 15

upper jaw incisor

weak

regional

weak expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6951
Entity Detected:	Cinp, cyclin-dependent kinase 2 interacting protein ( MGI:1914486)
Sequence:	sense strand is shown >T6951 CGGGTTTTTGGGTCTTCTCAGGAATCTACCTGTGGGCCGCCGCATGGAAGCAAAGACTCTTGGGATCGCA ACACCAAGAAAACCTGTCTTATCAGTCAGTGCGAGGAAACTTAAGGACAATGCTGCTGACTGGCATAACT TAATCCTGAAATGGGACAGCCTCAGTGACAAGGGCTTCACCACAGCGAGCAGTATTGCCAACCTGAAAGT CAGCTTGCTGAGTAAAGAGAAGGTTGAGTTAGAGAGCAGCAGCCCAGCTTCCATGGAAGAGGAAGAAAAG ACAAATCTGGACTACGATAAGGGACTCGAAGCACTGTGTGAGGAGCTGCAGGCCATCTTGGACGGCTTGA CTAAAATACAAATGAAAATGGAAAAGCTGTCTTCAACCACCAAGGGAATTTGTGAGCTAGAAAATTACCA CTACAGGGAAGAAAGCAGCCGGCCCCCTCTATTTCACACGTGGCCCACAGCCTTCTTTTATGAGGTCTCC CGCCGGCTCTCTGAAGCATACAAGAAGGAGCTCCTTTTGAAGCACACCATTGGCGCAGAGCTGGCCCATA CTGCAGATCGCAACCTCAGCCTGACCTACCTGTCCATGTGGCTGCACCAGCCCTACATAGAGAGCAACAG CAAGCTGCAGCTGGAGAGCATGCTGCTCGAGACAGGTCACCGGGCCCTGTGACTGCCTGTGACCACAGCT TGGAGCTCCAGGCCACTGGCTGGACAGTCAGTCCCTTGGGTGGAACCTGTTGTGCCTCAAGGCCAGATAC TTTGAGGGTCCTGGGATCACTCTTCCCGCTGCTGCAGCCTCTCCAGACACAGGCACTGCTGAGCTGTCCT CCCGCTGCTTCCCGGGTGTCATGAAGCTCCACTGAGTGACAGAAACAAGAACAGCTGAGGGCAGTTTTAT AAATCTGTGGAGACAACCTGAGTTTCCCATTTCTATTTTGTACCTATCTACTTTTTTTTTTCTTTTTTGT GGTGCTGGAAATCAAATCAAATCAAGACTTCACACAAGCTGAATATTCAACCACTGGTCCACACTGCCAA CCCTGTGCTTTGAAGAGAATGTTTTAGTTCTGAAACTAACTATCCCAGGATTATTAGATGTTAAAGTCTC ATAAGTAGGGACAGAGTGGTGAGTTAGCAGTTGAGAGCACTGTGGACTTCTGGCCTCCCCTGGCAGCAAC ACACAAATTACTTAGGTATCTGTGTATCGGCCGAATGTATAGATGACCAGGGCAACTGTAAATGGGAGTG GGCTCCTTGGAGTTAGAACTACAAGAGGTTGTCAGCCACCTGATTTGGGTGTTAGAAACCAAACTCCAGT CAGTCCTCTGGAAGAACAGTTCACGCTCTTAACTGCTGAGCCATCTCTCCTATGGCTGGAGATACATATA TTTAATATATTGTATAAATATATTAATAAAACATGTATTGAAATATAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3985202 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3985202 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7807 same embryo
	EMAGE:7810 same embryo
	EMAGE:7808 same embryo
	EMAGE:7809 same embryo
	EMAGE:7811 same embryo
	EurExpress:euxassay_005320 same experiment
	MGI:4823878 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source