EMAGE mouse gene expression spatial database

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EMAGE:8101

4930432K09Rik RIKEN cDNA 4930432K09 gene ( MGI:1921029)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016292. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016292_01	euxassay_016292_02	euxassay_016292_03	euxassay_016292_04

euxassay_016292_05	euxassay_016292_06	euxassay_016292_07	euxassay_016292_08	euxassay_016292_09

euxassay_016292_10	euxassay_016292_11	euxassay_016292_12	euxassay_016292_13	euxassay_016292_14

euxassay_016292_15	euxassay_016292_16	euxassay_016292_17	euxassay_016292_18	euxassay_016292_19

euxassay_016292_20	euxassay_016292_21	euxassay_016292_22	euxassay_016292_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8101_wholemount_strong.wlz

8101_wholemount_moderate.wlz

8101_wholemount_weak.wlz

8101_wholemount_possible.wlz

8101_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8101_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mandible

moderate

regional

moderate expression: see section 05 06 14 15 16 17 18 19 20 weak expression: see section 03 04 07 13

maxilla

moderate

regional

moderate expression: see section 05 06 14 17 weak expression: see section 04 07 13

lung

moderate

regional

moderate expression: see section 05 06 18 20 weak expression: see section 03 04 07 08 09 10 12 13 14 15 16 17 19 21

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 20 21 22 weak expression: see section 03 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45595
Entity Detected:	4930432K09Rik, RIKEN cDNA 4930432K09 gene ( MGI:1921029)
Sequence:	sense strand is shown >T45595 TCGCTACCCACTTAATCCATCTCTGAATATCCCGTTTGGCCTATGGAATGATAATTTGCCACCTTTTCTT TTGCCTCCTTTTAACACTCAACAAGGAAACACCATCACCAAATTCCCTGGAAGTTCTGAACTTGAAAAAG GGTTATCTCTATATCCTTGGATTGCCACTCCTTCTAAACTTCGCTATGTATACCAAAGTCTCAACTACCC AGCTGATGCTCCATTGAATGGTCCCCCAGTCGCCCCTCTTCCTCCTCCCAAACACTTTCCTCCCAGGCCA TACCCCTTTGTCATACCTCCAAAGATTTCTGTCATCTCACCTGTTAGACCTGAGCCCGTAGCAGTTCCTG CTATGCCTGCTGAAGGTGAGGGTCTGGTACCTGAGTTCCCTGTAGACAAACCTATTTTAGGTCTGCCTCA GGCAGTCAAACTTGGAACACCCGTGCCACCCTCCGGACCCAAACCTCTTGCACCCGAACCTGCTCCATCC CCGTTTGGTGCACCTGAGCCTGCCCCAGTCCAGTTTGGTGCACCTGAGCCTGCCCCCCACCAGTTAGAGG CAGCTGTCCCTGATGCATCCCGCCTCATGGCACCTGAACCCGCTGTACCCTTGTCTGTGGGAGCACAGTC ACTAGCTGAATCCCCTGCAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 201097. Forward Primer - name:201097_F_exon_4930432K09Rik, sequence:TCGCTACCCACTTAATCCATCT; Reverse Primer - name:201097_N_SP6_exon_4930432K09Rik, sequence:CCTGCAGGGGATTCAGCTAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8104 same embryo
	EMAGE:8103 same embryo
	EMAGE:8102 same embryo
	EMAGE:8100 same embryo
	EMAGE:8099 same embryo
	EurExpress:euxassay_016292 same experiment
	MGI:4822722 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source