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EMAGE:8104

Col6a4 collagen, type VI, alpha 4 ( MGI:1915803)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8104 EMAGE:8104 EMAGE:8104 EMAGE:8104 EMAGE:8104
"Pseudo-wholemount" of euxassay_016254. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016254_01 euxassay_016254_02 euxassay_016254_03 euxassay_016254_04
EMAGE:8104 EMAGE:8104 EMAGE:8104 EMAGE:8104 EMAGE:8104
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euxassay_016254_10 euxassay_016254_11 euxassay_016254_12 euxassay_016254_13 euxassay_016254_14
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euxassay_016254_15 euxassay_016254_16 euxassay_016254_17 euxassay_016254_18 euxassay_016254_19
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euxassay_016254_20 euxassay_016254_21 euxassay_016254_22 euxassay_016254_23 euxassay_016254_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8104Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8104_wholemount_strong.wlz
8104_wholemount_moderate.wlz
8104_wholemount_weak.wlz
8104_wholemount_possible.wlz
8104_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8104_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
esophagus
weak weak
regionalweak expression: see section 12
midgut
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 14 15 16 17 18 19 20 moderate expression: see section 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45533
Entity Detected:Col6a4, collagen, type VI, alpha 4 ( MGI:1915803)
Sequence:sense strand is shown

>T45533
AGAAGTTGGACATGGACTTGGTATTCCTGGTGGACAGCTCCCAGGGTGTCAGCAGGGACATATACCTTGG
GGCTTTGCGGCTAGTGGATTCAGTGCTCAAGGACCTGGAGGTGGCTGCGCAGCCTGGCACATCCTGGCAC
GGGGCGCGCGCAGCCCTGTTGACACACACAACCCCAGGCTTTTGGCCTGGTGTGGATCAAGCCCCTGTGC
TTGAGTACTTCCACCTGACCTCCCACGGCCACCGGACAGAAATGCAAAGGCAGATTCGGGAGGCTGCGAG
TGGCCTGCTACAGGGAGGCCCCGCCCTGGGCCACGCCCTGGAGTGGACGCTGGAAAACGTGCTTCTGACA
GCCGTACTGCCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 204267. Forward Primer - name:204267_F_exon_1110001D15Rik, sequence:AGAAGTTGGACATGGACTTGGT; Reverse Primer - name:204267_N_SP6_exon_1110001D15Rik, sequence:GAGGCAGTACGGCTGTCAGAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8103 same embryo
 EMAGE:8102 same embryo
 EMAGE:8101 same embryo
 EMAGE:8100 same embryo
 EMAGE:8099 same embryo
 EurExpress:euxassay_016254 same experiment
 MGI:4824426 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS