EMAGE mouse gene expression spatial database

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EMAGE:8196

Zfp819 zinc finger protein 819 ( MGI:1921650)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005255. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005255_01	euxassay_005255_02	euxassay_005255_03	euxassay_005255_04

euxassay_005255_05	euxassay_005255_06	euxassay_005255_07	euxassay_005255_08	euxassay_005255_09

euxassay_005255_10	euxassay_005255_11	euxassay_005255_12	euxassay_005255_13	euxassay_005255_14

euxassay_005255_15	euxassay_005255_16	euxassay_005255_17	euxassay_005255_18	euxassay_005255_19

euxassay_005255_20	euxassay_005255_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8196_wholemount_strong.wlz

8196_wholemount_moderate.wlz

8196_wholemount_weak.wlz

8196_wholemount_possible.wlz

8196_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8196_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8001
Entity Detected:	Zfp819, zinc finger protein 819 ( MGI:1921650)
Sequence:	sense strand is shown >T8001 AGCCAAGACATTCATGCTGCTGACTTTCTAGCACAACCAAGCTGAGTTCTGCCTGTCAGCTCTACCCAAA GGCTCCACATGCTCACCTGAATCCTGAGGGAATCCAGACATGGCTGCTGACATGAATTTCTGTCAGAAGC TCCAGAGACTGGGAACAGAGGAGCAGGACGGGCCGTGTGAGAGATTGGTGTCCTTTGAGGATGTAACTGT GAACTTCAGCCAGGAGGAGTGGCAGCACCTGGACTCCGCCCAGAGACGCCTGTACCAGGACGTGTTGCTG GAGATCTACAGCCACCTCTTGGTAGTGGGGTATTCCAGGCCAGAGGTCATCCTCAAGATGAAGAAAGTTA AGGACTCGCCAATGGGGAAGGTTGATTTCCCACATCAGAGGTGTGGTCAAGGGGAGGCAGAGCATGACTC ACACCAAATGTGTGTGAAGGCTGCATTTCCAAATGATGTATCAAGAGTAATCACTACAGGTGGCTCATAT TGTTCCATTATCGAGGAACCGTGGCAGGGTGGTGACCCTAAAACTTCAGATCCGCAAAACCACATCCCAC CCTCGAGTTCTGGTGCTTTCTTCAGCCAGAAAACACTAATCACAGATGGACGTGAAGCAGCTGGGGGATC CATTCCTTTAGGCGCCCACGTCATTTCTACACAAAAGAGATCTCTGAGATGTTGCCCGTGTGAAAAGACG TTGCAGCCTCACCTTCAAGCAGACAGAGACCATCAAAGCAGTGCCACAGAGCAGCCTGACGGCATTGTTG ACTCTTGTCAGTCCTTCACACAAGGCCTTCCCACTGCTGTGTGTCCAATTCACAGCACAGGAGAGGAAGC ACGCAGAGGGGACCAATGTGCAAGTGTTCCCAGCCCTCAGCAACCATTTATGCAGCATGGAACTCTTTCT CAGGACAAGCCAGTTGAATATACCAAATACGAGAAGGTCTTCACTGCCGGGTCAGCTTTGTGTCAGCAGC AAACAACTAACCCTCTAGAGACACATTTTATTTGCCACATATGTGGGAAATCCTTCCTTCAGAAATCTGA GTTGAGTTTTCATCCAGGAACAGACAGAGGAGAGACACATCATGGATGTCCTGGCTGTCGGAAATCACTT CCCAGTACATCCAGCCTGCAGGCACGGCATGGAACTCACACAAAGGAGAAACCTTACAGATGCCCTGACT GTGGGAAGTCATTCAGTTATGCATCCCACCTAAAGATCCATCTTCGAATACACACAGGTGAGAGGCCTTA TGTGTGTTCTGACTGTGGGAAGGCCTTTAGCCAAAAGTCAGTCCTCACCACGCATCGGAGAATCCACACT GGGGAGAGGCCTTACACATGTAACCACTGCGGGAAATTGTTTGTCTATGCATCAGATCTGAAGAAACACA GTCGGTTTCACACAGGGGAGAAGCCCTATGAGTGCAGAGATTGTGGGAAGTTATTCAATAATAAATCTCA CCTGCCTGTGCACTATCGCATTCACACCGGTGAGAAACCTTACAGATGCTGCGACTGTGGGAAGTCGTTC AGGAGAAAGTCCCACCTTAAAGTGCATAGCCGAACACACACTGGTGAGAAGCCTTATGAATGCCCTGACT GCGGAAGGGCCTTCAGCCACAGTTCAGTTCTCAGCACACATCAGAGAATTCACACTGGGGAGAGGCCTTA CCTATGCAGCGACTGTGGGAAGGCCATGTCTTCTAAAGCCCAGCTGAATGAGCACCGAAGGATCCACACG GGGGAGAAACCCTATGTGTGCAGCGAGTGTGGGAAAGCTTTCAGTTGCCGATCTTCCTTACAAGCACATC GTAGAACTCACAGTAGGGAAAAGCCGTTTGTCTGTCACACATGTGGGAAGGGCTTCCTGCACAAGTCACA GTTGTCATCTCACCAACAAACTCACACTGGTGAGAACCCTTAGGAGCGCTGTGCGTGTTGTGTGCAGTTG TTTGCTCATACGTATCAGCTGAAGGGACATCATCCGATTGAAGCAGAAGAAAATGACCTTGACTGAGAGA GTTCTCAACAGCTGAAGGCTCTCGTCTTTCATTCTGTGCAGAGAAACCTGAGGTGGGTTTTGAATGCAGA AAGTCCTCCAAACACAAGTCAGATTTCAGTAGACCCAGCAGACCTGACACTATAGATACTTGGACCTGTG AAAGGCTTTACCACAAAGTCAATTCCTCATCCATGTTTTTAAATAAAGAGACCTAATATGCATTGCTTAT TTTAAATACACAAATTATTATACCTGAGGAAAAGAATAAAGAATTTGTAAGAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:30007787 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30007787 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8195 same embryo
	EMAGE:8194 same embryo
	EMAGE:8198 same embryo
	EMAGE:8197 same embryo
	EurExpress:euxassay_005255 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source