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EMAGE:8250

Hnrnpul2 heterogeneous nuclear ribonucleoprotein U-like 2 ( MGI:1915943)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8250 EMAGE:8250 EMAGE:8250 EMAGE:8250 EMAGE:8250
"Pseudo-wholemount" of euxassay_007596. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007596_01 euxassay_007596_02 euxassay_007596_03 euxassay_007596_04
EMAGE:8250 EMAGE:8250 EMAGE:8250 EMAGE:8250 EMAGE:8250
euxassay_007596_05 euxassay_007596_06 euxassay_007596_07 euxassay_007596_08 euxassay_007596_09
EMAGE:8250 EMAGE:8250 EMAGE:8250 EMAGE:8250 EMAGE:8250
euxassay_007596_10 euxassay_007596_11 euxassay_007596_12 euxassay_007596_13 euxassay_007596_14
EMAGE:8250 EMAGE:8250 EMAGE:8250 EMAGE:8250 EMAGE:8250
euxassay_007596_15 euxassay_007596_16 euxassay_007596_17 euxassay_007596_18 euxassay_007596_19
EMAGE:8250 EMAGE:8250 EMAGE:8250
euxassay_007596_20 euxassay_007596_21 euxassay_007596_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8250Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8250_wholemount_strong.wlz
8250_wholemount_moderate.wlz
8250_wholemount_weak.wlz
8250_wholemount_possible.wlz
8250_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8250_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 05 06 07 14 15 16
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35345
Entity Detected:Hnrnpul2, heterogeneous nuclear ribonucleoprotein U-like 2 ( MGI:1915943)
Sequence:sense strand is shown

>T35345
CTTCCCTTCAAGTCCTCAGAGAGGCCTCTTATCAAGAGACTTCATTTGGGATCATATAGTTTGTAACTTT
AACAACACCCTCCCAGTTCTTGACAGAATAGCTACAGGTTGCTGGGTTCAAGAATGTGAGCAGGATATAG
CAATGCGCTTTCAATATTGATCTTCAATTTAAATCATCATCTTGCTTAGCTAAAAGCTTACTCAGCAACA
GCATCCTACAACACTTCCTGGTGAGAAAATCTCTGGGCTGAGTTACTAGGACATACTTCACTTCCACTTC
CTTGCCTGAGCAGTTCTCTTGTTTAAACTTGTGCTGAGATTTGAATCTTCCCTGTCCTGATGTCCTTCCC
ACGCAGGCAAGGCTTCTCAGGGTGTGCAGACCTCATTCTGAGTCCTGGTTTGTTAGGACACACCGTTCTG
CTCAAGGACCTCTTTCCAAGGCTGCAGTGGGCCTGGGAGGAGAGAAGCGTGCTCATACTGTGTCTTTTCT
ATTGCACTTGTCCCTATGGTGCCACAGATGGGTTTGTGTGTGGCAGTGGGTGGAATGATTGCATGCATCT
TTTGTAACTCGCTGGTATTAGAAAGGGAGTTCAAGAAAGCATTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85991. Forward Primer - name:085991_F_cDNA_1110031M08Rik, sequence:CTTCCCTTCAAGTCCTCAGAGA; Reverse Primer - name:085991_N_SP6_cDNA_1110031M08Rik, sequence:ACAATGCTTTCTTGAACTCCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8252 same embryo
 EMAGE:8251 same embryo
 EMAGE:8248 same embryo
 EMAGE:8249 same embryo
 EMAGE:8253 same embryo
 EurExpress:euxassay_007596 same experiment
 MGI:4825405 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS