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EMAGE:8253

1700055D18Rik RIKEN cDNA 1700055D18 gene ( MGI:1920655)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8253 EMAGE:8253 EMAGE:8253 EMAGE:8253 EMAGE:8253
"Pseudo-wholemount" of euxassay_007577. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007577_01 euxassay_007577_02 euxassay_007577_03 euxassay_007577_04
EMAGE:8253 EMAGE:8253 EMAGE:8253 EMAGE:8253 EMAGE:8253
euxassay_007577_05 euxassay_007577_06 euxassay_007577_07 euxassay_007577_08 euxassay_007577_09
EMAGE:8253 EMAGE:8253 EMAGE:8253 EMAGE:8253 EMAGE:8253
euxassay_007577_10 euxassay_007577_11 euxassay_007577_12 euxassay_007577_13 euxassay_007577_14
EMAGE:8253 EMAGE:8253 EMAGE:8253 EMAGE:8253 EMAGE:8253
euxassay_007577_15 euxassay_007577_16 euxassay_007577_17 euxassay_007577_18 euxassay_007577_19
EMAGE:8253 EMAGE:8253 EMAGE:8253 EMAGE:8253
euxassay_007577_20 euxassay_007577_21 euxassay_007577_22 euxassay_007577_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8253Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8253_wholemount_strong.wlz
8253_wholemount_moderate.wlz
8253_wholemount_weak.wlz
8253_wholemount_possible.wlz
8253_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8253_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35377
Entity Detected:1700055D18Rik, RIKEN cDNA 1700055D18 gene ( MGI:1920655)
Sequence:sense strand is shown

>T35377
AAGCAGTTTGGAAGAGGAAGTGTTTACTGGGCTTATCACCATGGTTTCGGAAAGTCCTGGGAAGAATTCA
AACAAACCGTAGACTAAAGCTACCTCCTGGCTTGGGCTCAGCTGTCCTTGTGCTCAGTTCACGCCTGCGT
GAGAATGGACAGGCCACAAGGGATGAGCCCTACTAAGTCAATTAATAAGCAAGCAATCCCTTTTCCCAAG
TGAAGGATAGTATAAAATTCTTAGTTGGTTCACAAGCTAATAGGGTTTCCTAGGGCTTCATCCATCCTTA
GTTTGCACCAGTTCCCAACCCCAATAAACCCATATAAAAACCACACAATCCAGTTATAAATATAAGCTAT
GTACCTAGACTGGACAGCTCTAGCACTGTGCTAACTTATTCCCCAGCTGCGAGACCCTTTGCTATTTGCA
GTTTCTCCTGGCCACGTGGTTCTGCTCCTCCTCTTCTTCAGTCCTCTCCCCTTCCCTTCTCCTCTCTACC
TGTCCCTACTCTCCAAGACCTCCCACCTCTCCCCTCCCCTGCCCAATTACAGGCCTCAGCCTTTATTGAC
CAGTTAAAATGGGGAAGAGGATGCCCTCAATACCCTCCCCTTTCCCTCATCCCAGTTTCCTTTCCAGATT
AACCCTTTAGCTCCTGAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68257. Forward Primer - name:068257_F_cDNA_1700055D18Rik, sequence:AAGCAGTTTGGAAGAGGAAGTG; Reverse Primer - name:068257_N_SP6_cDNA_1700055D18Rik, sequence:GTCAGGAGCTAAAGGGTTAATCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8252 same embryo
 EMAGE:8251 same embryo
 EMAGE:8248 same embryo
 EMAGE:8249 same embryo
 EMAGE:8250 same embryo
 EurExpress:euxassay_007577 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS