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EMAGE:8843

Ifnar1 interferon (alpha and beta) receptor 1 ( MGI:107658)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8843 EMAGE:8843 EMAGE:8843 EMAGE:8843 EMAGE:8843
"Pseudo-wholemount" of euxassay_010150. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010150_01 euxassay_010150_02 euxassay_010150_03 euxassay_010150_04
EMAGE:8843 EMAGE:8843 EMAGE:8843 EMAGE:8843 EMAGE:8843
euxassay_010150_05 euxassay_010150_06 euxassay_010150_07 euxassay_010150_08 euxassay_010150_09
EMAGE:8843 EMAGE:8843 EMAGE:8843 EMAGE:8843 EMAGE:8843
euxassay_010150_10 euxassay_010150_11 euxassay_010150_12 euxassay_010150_13 euxassay_010150_14
EMAGE:8843 EMAGE:8843 EMAGE:8843 EMAGE:8843 EMAGE:8843
euxassay_010150_15 euxassay_010150_16 euxassay_010150_17 euxassay_010150_18 euxassay_010150_19
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euxassay_010150_20 euxassay_010150_21 euxassay_010150_22 euxassay_010150_23 euxassay_010150_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8843Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8843_wholemount_strong.wlz
8843_wholemount_moderate.wlz
8843_wholemount_weak.wlz
8843_wholemount_possible.wlz
8843_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8843_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32050
Entity Detected:Ifnar1, interferon (alpha and beta) receptor 1 ( MGI:107658)
Sequence:sense strand is shown

>T32050
TCCACATCTCTCCTCCCGGACAAGACGGGAACATGTGGGCACTGGAGAAACCTTCCTTCAGTTACACCAT
ACGAATCTGGCAGAAGTCTTCCAGTGACAAAAAAACTATTAACTCTACGTATTATGTAGAAAAGATACCA
GAACTCTTGCCAGAGACTACTTACTGTTTAGAAGTTAAAGCAATACATCCGTCACTTAAGAAACACAGCA
ATTACAGCACTGTGCAGTGTATAAGCACCACAGTGGCAAATAAAATGCCTGTGCCAGGAAATCTCCAAGT
GGATGCCCAAGGCAAGAGCTATGTCCTGAAATGGGACTACATTGCGTCTGCAGACGTGCTCTTCAGGGCA
CAGTGGCTTCCTGGCTATTCAAAAAGCAGTTCTGGAAGCCGTTCAGATAAATGGAAACCAATACCAACCT
GTGCAAATGTCCAGACTACGCACTGTGTCTTTTCTCAAGATACTGTCTACACAGGAACGTTCTTTCTCCA
TGTACAAGCCTCAGAGGGAAATCACACATCCTTTTGGTCTGAAGAGAAGTTTATTGATTCTCAAAAACAC
ATTCTCCCTCCTCCTCCGGTCATTACTGTCACCGCCATGAGTGACACCTTGCTTGTTTATGTCAACTGTC
AGGACAGCACATGTGATGGACTCAATTACGAAATCATCTTTTGGGAAAACACTTCCAATACTAAGATAAG
CATGGAGAAGGATGGCCCAGAGTTCACCCTCAAGAACCTGCAGCCGCTGACTGTGTACTGTGTCCAGGCC
AGAGTGCTCTTCAGGGCCCTGCTGAATAAGACCAGCAACTTCAGTGAAAAGCTGTGTGAGAAAACACGTC
CAGGAAGTTTTTCCACGATCTGGATTATAACTGGATTAGGTGTTGTGTTCTTCTCTGTCATGGTCCTTTA
TGCTTTGAGGAGCGTCTGGAAATACCTGTGTCATGTGTGCTTCCCACCACTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4507009), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57944. Forward Primer - name:057944_F_IRAV88_e07_Ifnar1, sequence:TCCACATCTCTCCTCCCG; Reverse Primer - name:057944_R_SP6_IRAV88_e07_Ifnar1, sequence:TGAGTGGTGGGAAGCACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8845 same embryo
 EMAGE:8844 same embryo
 EMAGE:8847 same embryo
 EMAGE:8846 same embryo
 EMAGE:8848 same embryo
 EurExpress:euxassay_010150 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS