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EMAGE:8851

Sgpp2 sphingosine-1-phosphate phosphotase 2 ( MGI:3589109)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8851 EMAGE:8851 EMAGE:8851 EMAGE:8851 EMAGE:8851
"Pseudo-wholemount" of euxassay_010095. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010095_01 euxassay_010095_02 euxassay_010095_03 euxassay_010095_04
EMAGE:8851 EMAGE:8851 EMAGE:8851 EMAGE:8851 EMAGE:8851
euxassay_010095_05 euxassay_010095_06 euxassay_010095_07 euxassay_010095_08 euxassay_010095_09
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euxassay_010095_10 euxassay_010095_11 euxassay_010095_12 euxassay_010095_13 euxassay_010095_14
EMAGE:8851 EMAGE:8851 EMAGE:8851 EMAGE:8851 EMAGE:8851
euxassay_010095_15 euxassay_010095_16 euxassay_010095_17 euxassay_010095_18 euxassay_010095_19
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euxassay_010095_20 euxassay_010095_21 euxassay_010095_22 euxassay_010095_23 euxassay_010095_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8851Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8851_wholemount_strong.wlz
8851_wholemount_moderate.wlz
8851_wholemount_weak.wlz
8851_wholemount_possible.wlz
8851_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8851_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32118
Entity Detected:Sgpp2, sphingosine-1-phosphate phosphotase 2 ( MGI:3589109)
Sequence:sense strand is shown

>T32118
TGACCTACCCTGCCTGGACCCTCATCGACTCCCTGGACTCTGCCAGCCCCCTCTTCCCTGTGTGTGTCAT
AGTTGTACCGTTCCTCTTGTGCTACAATTACCCTGTGTCTGACTACTACAGCCCGACCAGGGCAGACACC
ACCACCATCGTGGCTGCTGGGGCTGGAGTGACCCTGGGATTCTGGATCAACCATTTCTTCCAGCTCGTGT
CCAAGCCCACCCCATCTCTTCCTGTCATTCAGAACATCCCACCCCTTACCACTGACATGCTGGTTTTAGG
TCTGACCAAATTTATGGTGGGAATCATGTTGATCCTCTTGGTTCGCCAACTGGTGCAAAAGCTCTCCCTG
CAAGTATTATTCTCATGGTTCAAGGTGGTTACTAGGAACAAGGAGGCCAGGCGAAGACTAGAGATTGAAG
TCCCTTACAAGTTTGTTACCTACACATCTGTTGGCATCTGTGCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6399201), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58517. Forward Primer - name:058517_F_IRAV93_b09_MGC59240, sequence:TGACCTACCCTGCCTGGA; Reverse Primer - name:058517_R_SP6_IRAV93_b09_MGC59240, sequence:GTGGCACAGATGCCAACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8849 same embryo
 EMAGE:8852 same embryo
 EMAGE:8854 same embryo
 EMAGE:8853 same embryo
 EMAGE:8850 same embryo
 EurExpress:euxassay_010095 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS