EMAGE mouse gene expression spatial database

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EMAGE:10185

Mmp17 matrix metallopeptidase 17 ( MGI:1346076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000007. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000007_01	euxassay_000007_02	euxassay_000007_03	euxassay_000007_04

euxassay_000007_05	euxassay_000007_06	euxassay_000007_07	euxassay_000007_08	euxassay_000007_09

euxassay_000007_10	euxassay_000007_11	euxassay_000007_12	euxassay_000007_13	euxassay_000007_14

euxassay_000007_15	euxassay_000007_16	euxassay_000007_17	euxassay_000007_18	euxassay_000007_19

euxassay_000007_20	euxassay_000007_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10185_wholemount_strong.wlz

10185_wholemount_moderate.wlz

10185_wholemount_weak.wlz

10185_wholemount_possible.wlz

10185_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10185_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

caudate-putamen

weak

regional

weak expression: see section 09 10 11

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 11 14 weak expression: see section 15

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 14

thalamus mantle layer

moderate

homogeneous

moderate expression: see section 11 weak expression: see section 15

thalamus ventricular layer

moderate

regional

moderate expression: see section 13

cerebral cortex mantle layer

strong

homogeneous

strong expression: see section 21 moderate expression: see section 01 02 03 04 06 07 08 09 17 18 19 20 weak expression: see section 10 11 16

lentiform nucleus

weak

regional

weak expression: see section 08 09 10 11 16 17

telencephalic part of interventricular foramen

moderate

regional

moderate expression: see section 13 weak expression: see section 14

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 12 16

medulla oblongata alar plate marginal layer

moderate

regional

moderate expression: see section 17

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 07 11 14 17

metencephalon rest of alar plate mantle layer

moderate

regional

moderate expression: see section 05 06 07 08

midbrain ventricular layer

weak

homogeneous

weak expression: see section 11 12 14 15

tegmentum

moderate

single cell

moderate expression: see section 11 14

basal columns

moderate

single cell

moderate expression: see section 09 11 12

intermediate grey horn

moderate

single cell

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2195
Entity Detected:	Mmp17, matrix metallopeptidase 17 ( MGI:1346076)
Sequence:	sense strand is shown >T2195 CCCACCTGTCAGACTAGGCAGGACAGAGTCAGGGGTAGGGGCATCTGAGGTTTCCCTGTCTTGGAAGCCA CCCTACTCTGCCCTCATATCAAAGCACGCTCCTATGATGTCCCATGTTGTCCACCAGCCTGCAGGACACA GATGTCCTATACAGCAACAGGGAAGGGTCCAAAAATCTTTGTCACATAGCACTGAAAGCCAGGCCCGCAG GCTGGAGCTGTCTAGATGCTGGTGTCACACTCATTTTAAAACCCAAACTCTTAATAAAAATTTTGTACAC TGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:904649 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:904649 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10184 same embryo
	EMAGE:10183 same embryo
	EMAGE:10182 same embryo
	EurExpress:euxassay_000007 same experiment
	MGI:4826408 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source