EMAGE mouse gene expression spatial database

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EMAGE:10423

Arfgap1 ADP-ribosylation factor GTPase activating protein 1 ( MGI:2183559)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000537_01	euxassay_000537_02	euxassay_000537_03	euxassay_000537_04

euxassay_000537_05	euxassay_000537_06	euxassay_000537_07	euxassay_000537_08	euxassay_000537_09

euxassay_000537_10	euxassay_000537_11	euxassay_000537_12	euxassay_000537_13	euxassay_000537_14

euxassay_000537_15	euxassay_000537_16	euxassay_000537_17	euxassay_000537_18	euxassay_000537_19

euxassay_000537_20	euxassay_000537_21	euxassay_000537_22	euxassay_000537_23	euxassay_000537_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10423_wholemount_strong.wlz

10423_wholemount_moderate.wlz

10423_wholemount_weak.wlz

10423_wholemount_possible.wlz

10423_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10423_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

inner ear

strong

regional

strong expression: see section 20

labyrinth

moderate

regional

moderate expression: see section 21

otic capsule

strong

regional

strong expression: see section 20 moderate expression: see section 22 23 24 weak expression: see section 16

mandible

strong

regional

strong expression: see section 18 moderate expression: see section 17 weak expression: see section 16

lower jaw mesenchyme

weak

regional

weak expression: see section 16

axial skeleton

moderate

regional

moderate expression: see section 23 24

cranium

moderate

regional

moderate expression: see section 24

basioccipital bone

strong

regional

strong expression: see section 22 moderate expression: see section 17 21 23

basisphenoid bone

strong

regional

strong expression: see section 20 22 23 moderate expression: see section 17 18

frontal bone primordium

strong

regional

strong expression: see section 22 23 moderate expression: see section 21

inter-parietal bone primordium

strong

regional

strong expression: see section 22 23

parietal bone

strong

regional

strong expression: see section 20 22 moderate expression: see section 21

optic foramen

moderate

regional

moderate expression: see section 18

orbito-sphenoid

moderate

regional

moderate expression: see section 17 weak expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3781
Entity Detected:	Arfgap1, ADP-ribosylation factor GTPase activating protein 1 ( MGI:2183559)
Sequence:	sense strand is shown >T3781 TGGCCTCGAGCCAGATTCGGACGAGGGGGGATCTGAGCGCCTAGTCCAGCTTGGCACCTCGGCCGGACCC GACCTCCTGGGGGCAGCAGGATCCCAGTGTGCGGCCGTCCAGACCCCCCCTCCCCTGCCTCCCGGCCTGT GAGCTTTACCGCCCTTCAGCCTAGCGGTTGCAGGGACCTACGTGGCCCCTCAGCGCTCTGGTCCCGAGCA GTCTTGGACGCAGACTGGTTCTGCACTGCCCACTTTGTCGGGAAAGGGCCTCAGCCCTTCACTTCGAAAG AAGATTCTGTCCAGAGGTGCCGCACCATGGCCAGCCCAAGAACCAGGAAAGTTCTTAAGGAAGTCCGGGC ACAGGATGAGAATAATGTTTGCTTTGAGTGTGGTGCGTTCAATCCTCAGTGGGTCAGCGTGACTTATGGC ATCTGGATCTGCCTGGAATGCTCTGGGAGACACCGTGGGCTTGGGGTGCACCTCAGCTTTGTGCGCTCTG TTACAATGGACAAATGGAAGGACATTGAGCTGGAGAAGATGAAGGCTGGTGGGAATGCT
Notes:	The probe template was PCR amplified from IMAGE:390020 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:390020 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10426 same embryo
	EMAGE:10422 same embryo
	EMAGE:10425 same embryo
	EMAGE:10424 same embryo
	EMAGE:10421 same embryo
	EurExpress:euxassay_000537 same experiment
	MGI:4823210 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source