EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10569

Sfi1 Sfi1 homolog, spindle assembly associated (yeast) ( MGI:1926137)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000826. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000826_01	euxassay_000826_02	euxassay_000826_03	euxassay_000826_04

euxassay_000826_05	euxassay_000826_06	euxassay_000826_07	euxassay_000826_08	euxassay_000826_09

euxassay_000826_10	euxassay_000826_11	euxassay_000826_12	euxassay_000826_13	euxassay_000826_14

euxassay_000826_15	euxassay_000826_16	euxassay_000826_17	euxassay_000826_18	euxassay_000826_19

euxassay_000826_20	euxassay_000826_21	euxassay_000826_22	euxassay_000826_23	euxassay_000826_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10569_wholemount_strong.wlz

10569_wholemount_moderate.wlz

10569_wholemount_weak.wlz

10569_wholemount_possible.wlz

10569_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10569_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

visceral pericardium

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

main bronchus cartilage

strong

regional

strong expression: see section 12 13

limb

strong

regional

strong expression: see section 01 02 03 04 05 07 moderate expression: see section 24

gut

strong

regional

strong expression: see section 14 15 moderate expression: see section 13 16 17 18 19 20

dermis

moderate

homogeneous

moderate expression: see section 01 02 03 04 06 08 14

perioptic mesenchyme

moderate

homogeneous

moderate expression: see section 02 03 04 05

foregut duodenum

strong

regional

strong expression: see section 12

gastro-esophageal junction

strong

regional

expression in the associated mesenchyme.

esophagus

strong

regional

expression in the associated mesenchyme.

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12

palatal shelf mesenchyme

strong

regional

strong expression: see section 10 16 moderate expression: see section 09

liver

strong

regional

strong expression: see section 01 02

detrusor muscle of bladder

moderate

homogeneous

moderate expression: see section 14 15 16 17

metanephros

weak

homogeneous

weak expression: see section 08 09 10 18 19 20

left lung mesenchyme

strong

regional

strong expression: see section 06 09 10 11 12

left lung lobar bronchus mesenchyme

strong

regional

strong expression: see section 05 06 07 08 11 12

right lung mesenchyme

moderate

regional

moderate expression: see section 14 15 16 17 18

right lung caudal lobe lobar bronchus mesenchyme

moderate

regional

moderate expression: see section 14 16 17 18

right lung caudal lobe lobar bronchus epithelium

moderate

regional

moderate expression: see section 15

right lung cranial lobe lobar bronchus mesenchyme

moderate

regional

moderate expression: see section 14 15 16 17 18

main bronchus mesenchyme

moderate

regional

moderate expression: see section 15

trachea mesenchyme

moderate

regional

moderate expression: see section 14

trachea cartilaginous ring

strong

regional

strong expression: see section 13

skeleton

strong

regional

strong expression: see section 05 moderate expression: see section 22 23 24

axial skeleton

strong

regional

strong expression: see section 06 07 09 10 12 13 14 16 17 18 moderate expression: see section 15

cranium

strong

regional

strong expression: see section 06 07 19

pectoral girdle and thoracic body wall skeleton

strong

regional

strong expression: see section 09 10 11 16 17 18 19

pelvic girdle skeleton

strong

regional

strong expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3915
Entity Detected:	Sfi1, Sfi1 homolog, spindle assembly associated (yeast) ( MGI:1926137)
Sequence:	sense strand is shown >T3915 GCCCTTTGCTACCACCTCTGTCTAGCGTCTGCTGAGGAGTCACCTCCCTCAGTTATAAGAGGAAGTTAAA AGGCTTTGGTTCATCAATAACCTAGTCACAAAAATACATATCAACCACTGGTTGAATTTCCATCAAGAGG AAAGAATGGAGAAGAAGATTGGTTCCAGGTCTTTTAGGGATGGTGTGGTTAAGAAACCTTGTTCTCCAAA GACCCTGCCCCTCAAAAAGTCTTCTGCCTTCTCTGGGATCCAGAGGGAGCCATCTAGAAGCTGCCACTCT ATATACTATCATGCCTCACAAAACTGGACTCGGTACCGATTACAAGAACTTCGAATCAGGTGTGTGGCCA GAAAGTTCCTGTATTTGTGGATTCGAGTGACTTTTGGAAGAGTGACTCCCTCTAGAGCCAGGATTTTCCA TGAGCAGAAAATATTACAGAAGGTCTTCGGAGAATGGAGGGAAGAATGGTGGGTCTCCCAGAGGGAGTGG AAGCTCTGTGTCCGAGCTGACTGTCACTACAGATACTACTTGTACAACCTGATTTTCCAGAACTGGAAGA CCTTTGTGCACCAGCAACGGGAGATGAGGAAGAGGTTCCGAATAGCTGAGCATCATGATACGAAGCAGAA GATGTGCCAAGCCTGGAAGTCCTGGTTGATATACATGGTTTCTCGTAGGACCAAACTTCACATGAAGACT ACTGCTCTAGAGTTTAGGAGGCAGAGTGTCTTATGGGTGACAGTCCTACACAAGTGTGTCAGGGTGTGGC TACGGTATGTGCACAAGAGACAATGGCAGCAGTTATTGCGGGCCAGAGCAGATGGTCATTTCCAGCAGCG AGCACTGCCTGCTGCCTTCTACACGTGGTACAGAGGCTGGCTATGGCACCAGCAGAGACGGATCCTGCAC ACAAAAGCAGTGCGTTTCCACAGAGGGACATTAGAGAAGCAAGTATTTGCTCTCTGGAGGCAGAAGATGT CTCAGCATCGAGAAAACTGCTTGGCAGAGAGAATGGCTATACTGCAGGCAGAGCAGCAGCTTTTGCGTAG GTTCTGGTTCGTGTGGCACCAGCAGGCAGCAGTGTGTCAGCTGGAGCGACAGCAGCAAGCCATGGCCATT GCCCACCACCACAGTGGACTGCTCAGGAGGGCTTTCTGCATCTGGAAGGAAAGCACCCAAGGATTTAGGA TAGAGAGGATGGGCAGGGCACAGGCTGCGCACTTCCACTCTGCACAGCTCTTGAGTCGGGCCTGGAGCAT GTGGAGGGAGGTTTACCAGAATAGGGTGAGGAGTGTTCTGCGGGAGGTGGCGGCCAGGGAGAGACAGCAC AACAGGCAGCTGCTGTGGTGGGCGTTACATCTCTGGAGAGAAAACACCATGGCCCGTTTGGATGGAGCCA AAAAAACCTCTCAAGCAAGAGTTCACTACAGCAGGACTCTGTGCTCCAAGGTCCTCGTCCAGTGGCGAGA GGTTACTTCAGTGCAGATATATTACCGACAAAAGGAGGCAGCTGCCCTCAGGGAGGCCCGGAAAGCACTG GACAGGGGTCGTCTCCAGAACTGGTTTCAGCACTGGCGATTCTGCAGCCAGAGGGCAGCCCAGCAGAGAT TCCAGCTGGGACAGGCGGCTCAGCATCACCACTGGCAGCTGCTGATGGAAGCAATGGCCAGGTGGAAGGC ACACCATCTGGGGTGTATCAGAAAGAAGTTTCTGCAGAGGCAAGCTGCCCAACTCCTGGCACAGAGACTC AGTCGGGCCTGCTTCTGCCAGTGGAGGAAGCAGCTGGCAGTCCGGAAGCAAGAACAGTGGGGCACAGCCC GGGCCCTGTGGCTCTGGGCCTTCTCACTACAAGCAAAGGTGTGGACTGCATGGCTGGGCTTCGTACTTGA GAGGAGGAGGAAGAAGGCACGGCTGGAACGGGCTATGCAAGCCTACCAGCAGCAGCTCCTACAGGAGGGT GCCACACGGCTTCTGCGCTTCACAGCTGGTACGAAGGCCTTCCGGCAGCAGCTCCAGGCCCAGCAGCAGG TCCAGGCAGCCCACAGCCTCCACTGTGCAGTCCGCCACTGTGCTGAGCTCTGGAAAAAGAAGGTGCTTGG TCCAGGCAAGACTTCTCAACCACCAGCACCCACCACATTCAGCAAGAGAGTGACATTTAAGGATTCTTTC CTCTCTGGACATGCTGCGGAGGCTGGAGATGCTACCCAGGAGACAAAGAAGCTTCGAGCTCCTCCATCTC AGGGAGTCCTGGGTAGTTTGGCAGGAGCTGCTGGGGAGCCCTGCCACCTAGACCTGTGAGTGGACTGTGG TTCACCTTTGCCTCTAGTCCTAGAGCCAAAGCTGGGCACACACAAGAGGGACAAACAGCTGTAGGAAGGA CAAGATGAGAGTGCTACAGCCTCATCACATCAGAGCTGCTCAGGGGCCTGTGTCAGACCTTTCTTCCCTG GGCATTGTTCTGAGGCAATTGTCATTCCCAGAGTAACCAGCTCTGAGCTCAGAACTCATGGTGGGTAGAG TGTTCTGGACGAGCCCCTACTTGTCCCCACTCTTTACCCCTCACCGCTAACCTTGGCAGGACCCTGAATA TCTCAGAGGGTCTTCTGATAGGAAAGAACACATACCATCAGCTTGCTGTGAAGGTCAAAAGAAACAGGTG TAGCTCCAGCACCCAGGAATCAGTCAGGAAGGCTGTAAGGGTCATGTTGAGTTGTCTAGGCTCAGACCTA CCCTGTGCACATGCTGGCCTTCAGTCTCCCTGTAAAATGTCAAGGACTGAAGCTTAGCGAGATCTCAGGT GCCCTTGCAGACCTTGTCAGTCCGAGGAACAGACTATTTCTCTCCAGCAATGCAGCCCGCTCATCACGGA AACAGCCCCGACGTCCATCCTTCCTGCTTGAGCGTTTGGGGAGTCAGAGGTCCCCAGAGTGGTACAGCCT TGGGGAGCAGCAGTTAGAGAAGCCCCCAGAGGAGGAAAGCACGGCTCTGCTGGGAGGCTCTTCCCTGACA AGACCCTTCCTACCTGGGGTCCTGCCGAATGTTCCTGGTCCGAAGCTGCCTCCTACGGCCAGTCCAGGCC TGGAGCTGCTGCCTCCCTCCTCCATCATGCCCCATGCGGCAGGAGGCACTGCTAGGGTGTCAGCGAAGCC CAGCATCCCTGGGCCTCAGCCTTGGGGGTGCCCGTCCTTACCCAGGGACCTTGATCCCCAACTCCTCCCT GGAGATTCCATAAGCACCCGGACTGAGCCTGTTTATGGCTCAGAGGCTACAGGCCACACAGAGCTTGAGG CTGAGTTGGAGGGGATCCAGCAGCAGTTACAACACTACCAAACCACCAAGCAGAACCTTTGGTCATGCCA GCGACAAGCCAACAGCCTGCGTAGGTGGCTGGAGCTAAGCCAGGAGGAGCCGAAATCCGAGGACCTTCAC CTAGAAGAGCAGGTGAAAACAGAACTGGAGGAGGTGGAACTACAGGTCCAGCAGCTGGCTAAGGAGCTTG AGGCCCAGCGTCAGCCTGTTGGTACATGCATTGCTCGTGTTCGGGCCCTGAGACGGGCACTGTGCTAGTG GCCCGTCCCTGGGGGATAGGTGCTATCCTTCACAAGGCCTGGGACACCCTCAACAACAAAATTCAAAGCT ATGGTAGTTTTTGTTTTGTTTTGTTTTTTAATTTCAAAATGTCTTGCAATGAAATGAATTACTGTTAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6493676 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6493676 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10571 same embryo
	EMAGE:10570 same embryo
	EMAGE:10568 same embryo
	EurExpress:euxassay_000826 same experiment
	MGI:4828010 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source