EMAGE mouse gene expression spatial database

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EMAGE:10644

Por P450 (cytochrome) oxidoreductase ( MGI:97744)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003208. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003208_01	euxassay_003208_02	euxassay_003208_03	euxassay_003208_04

euxassay_003208_05	euxassay_003208_06	euxassay_003208_07	euxassay_003208_08	euxassay_003208_09

euxassay_003208_10	euxassay_003208_11	euxassay_003208_12	euxassay_003208_13	euxassay_003208_14

euxassay_003208_15	euxassay_003208_16	euxassay_003208_17	euxassay_003208_18	euxassay_003208_19

euxassay_003208_20	euxassay_003208_21	euxassay_003208_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10644_wholemount_strong.wlz

10644_wholemount_moderate.wlz

10644_wholemount_weak.wlz

10644_wholemount_possible.wlz

10644_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10644_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 01 02 03 04

hindlimb digit 1 phalanx

moderate

regional

moderate expression: see section 05 06 22

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 05

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 05 06 22

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 05

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 06 07 08 22

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 07 08 22

adrenal medulla

strong

regional

strong expression: see section 10 11 12 19 20 21

thymus primordium

moderate

regional

moderate expression: see section 13 14 15 16 17

sublingual gland primordium

weak

regional

weak expression: see section 11 16 17

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 18 19 20 21

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 10 11 12 15 16 17 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 09 20

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 10 19

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 12 18

cervical ganglion

weak

regional

weak expression: see section 11 18

thoracic ganglion

weak

regional

weak expression: see section 15 16

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 13 14 18 19

neural retina

moderate

regional

moderate expression: see section 02 weak expression: see section 01 03 04

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 15 16 moderate expression: see section 08 09 17 18 19 20

lower jaw incisor

weak

regional

weak expression: see section 12 13 15 16 17

lower jaw molar

weak

regional

weak expression: see section 08 09

upper jaw incisor

weak

regional

weak expression: see section 11 12 13 15 16 17

upper jaw molar

weak

regional

weak expression: see section 08 09

metanephros

weak

regional

weak expression: see section 10 11 12 20 21 22

testis

strong

regional

strong expression: see section 07 08 09 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6186
Entity Detected:	Por, P450 (cytochrome) oxidoreductase ( MGI:97744)
Sequence:	sense strand is shown >T6186 GCGCGCGGTGTGTGATCTGGTCGGTACCGAGGAGCGCAGGTTGTGTCACCAACATGGGGGACTCTCACGA AGACACCAGTGCCACAGTGCCTGAGGCAGTGGCTGAAGAAGTGTCTCTATTCAGCACAACGGACATTGTT CTGTTTTCTCTCATCGTGGGGGTCCTGACCTACTGGTTCATCTTTAAAAAGAAGAAAGAAGAGATACCGG AGTTCAGCAAGATCCAGACAACGGCCCCACCTGTCAAAGAGAGCAGCTTCGTGGAAAAGATGAAGAAAAC GGGAAGGAACATTATTGTATTCTATGGCTCCCAGACGGGAACCGCGGAGGAGTTTGCCAACCGGCTGTCC AAGGATGCCCACCGCTATGGGATGCGGGGCATGTCTGCAGACCCTGAAGAGTATGACTTGGCCGACCTGA GCAGCCTGCCTGAGATCGACAAGTCCCTGGTAGTCTTCTGCATGGCCACATACGGAGAAGGCGACCCCAC CGACAACGCGCAGGACTTCTATGATTGGCTGCAGGAGACTGACGTGGACCTCACGGGTGTCAAGTTTGCT GTGTTTGGTCTCGGGAACAAGACCTATGAGCACTTCAACGCCATGGGCAAGTATGTGGACCAGCGGCTGG AGCAGCTTGGCGCCCAGCGAATCTTTGAGTTGGGCCTTGGTGATGACGACGGGAACTTGGAAGAGGATTT CATCACATGGAGGGAGCAGTTCTGGCCAGCTGTGTGCGAGTTCTTCGGGGTGGAAGCCACTGGGGAGGAG TCGAGCATCCGCCAGTACGAGCTCGTGGTCCACGAAGACATGGACACAGCCAAGGTGTACACGGGTGAGA TGGGCCGTCTGAAGAGCTACGAGAACCAGAAACCCCCCTTCGATGCCAAGAATCCATTCCTGGCTGCTGT CACCACGAACCGGAAGCTGAACCAAGGCACTGAGAGGCATCTAATGCACCTGGAATTGGACATCTCAGAC TCCAAGATCAGGTATGAATCTGGAGATCACGTGGCTGTGTACCCAGCCAACGACTCCACCCTGGTCAACC AGATTGGGGAGATCCTGGGGGCTGACCTGGATGTCATCATGTCTCTAAACAATCTCGATGAGGAGTCGAA TAAGAAGCATCCGTTCCCCTGCCCCACCACCTACCGCACGGCCCTCACCTACTACCTGGACATCACTAAC CCGCCACGAACCAACGTGCTCTACGAGCTGGCCCAGTACGCCTCAGAGCCCTCGGAGCAGGAACACCTGC ACAAGATGGCGTCCTCCTCCGGCGAGGGCAAGGAGCTGTACCTGAGCTGGGTGGTGGAGGCCCGGAGGCA CATCCTAGCCATTCTCCAAGACTACCCGTCCCTGCGGCCACCCATCGACCACCTGTGCGAGCTCCTCCCG AGGCTGCAGGCCCGCTACTATTCCATTGCCTCGTCGTCTAAGGTCCACCCCAACTCCGTGCACATCTGCG CCGTGGCTGTGGAGTATGAAGCGAAGTCTGGACGAGTGAACAAGGGGGTGGCCACCAGCTGGCTTCGGAC CAAGGAACCAGCAGGAGAGAATGGCCGCCGGGCCCTGGTCCCCATGTTCGTCCGCAAGTCCCAGTTCCGC TTGCCTTTCAAGCCCACCACACCTGTTATCATGGTGGGCCCCGGCACTGGGGTTGCCCCTTTCATGGGCT TCATCCAGGAGCGGGCTTGGCTTCGAGAGCAAGGCAAGGAGGTCGGAGAGACGCTGCTCTACTACGGCTG CCGGCGCTCGGATGAGGACTATCTGTACCGCGAGGAGCTGGCGCGCTTCCACAAGGACGGCGCCCTCACG CAGCTTAATGTGGCCTTTTCCCGTGAGCAGGCCCACAAGGTCTATGTTCAGCACCTGCTCAAGAGGGACA AAGAGCACCTGTGGAAGCTGATCCACGAAGGTGGTGCCCACATCTATGTCTGCGGGGATGCTCGAAATAT GGCCAAAGATGTGCAGAACACATTCTATGACATCGTGGCCGAGTTTGGGCCCATGGAGCACACCCAGGCT GTGGACTATGTTAAGAAGCTCATGACCAAGGGCCGCTACTCGCTGGATGTATGGAGCTAGGAGCTGCCGC CCCCCACCCCTCGCTCCCTGTAATCACGTCCTTAACTTCCTTCTGCCGACCTCCACCTCTGGTGGTTCCT GCCCTGCCTGGACACAGGGAGGCCCAGGGACTGACTCCTGGCCTGAGTGATGCCCTCCTGGGCCCTTAGG CAGAGCCTGGTCCATTGTACCAGGCAGCCTAGCCCAGCCCAGGGCACATGGCAAGAGGGACTGGACCCAC CTTTGGGTGATGGGTGCCTTAGGTCCCCAGCAGCTGTACAGAAGGGGCTCTTCTCTCCACAGAGCTGGGG TGCAGCCCCAACATGTGATTTTGAATGAGTGTAAATAATTTTAAATAACCTGGCCCTTGGAATAAAGTTG TTTTCTGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497979 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497979 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10645 same embryo
	EMAGE:10643 same embryo
	EMAGE:10646 same embryo
	EMAGE:10647 same embryo
	EurExpress:euxassay_003208 same experiment
	MGI:4827335 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source