EMAGE mouse gene expression spatial database

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EMAGE:10715

Sssca1 Sjogren's syndrome/scleroderma autoantigen 1 homolog (human) ( MGI:1913482)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001279. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001279_01	euxassay_001279_02	euxassay_001279_03	euxassay_001279_04

euxassay_001279_05	euxassay_001279_06	euxassay_001279_07	euxassay_001279_08	euxassay_001279_09

euxassay_001279_10	euxassay_001279_11	euxassay_001279_12	euxassay_001279_13	euxassay_001279_14

euxassay_001279_15	euxassay_001279_16	euxassay_001279_17	euxassay_001279_18	euxassay_001279_19

euxassay_001279_20	euxassay_001279_21	euxassay_001279_22	euxassay_001279_23	euxassay_001279_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10715_wholemount_strong.wlz

10715_wholemount_moderate.wlz

10715_wholemount_weak.wlz

10715_wholemount_possible.wlz

10715_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10715_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 17 18 19 20 21

pancreas

weak

regional

weak expression: see section 10 11 12 13 14 15 17 18 19

renal cortex

weak

regional

weak expression: see section 09 10 11 12 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4917
Entity Detected:	Sssca1, Sjogren's syndrome/scleroderma autoantigen 1 homolog (human) ( MGI:1913482)
Sequence:	sense strand is shown >T4917 ACGGCAACATGGCCTTGAACGGTGCAGATGTCGACGATTTCACCTGGGAGCCTCCGACTGAAGCTGAGAC GAAGGTGCTGCAGGCGCGACGGGAGCGGCAGGATCGCATCTCCCGTCTGATGGGGGACTACCTGCTCCGT GGTTACCGCATGCTGGGCGACACGTGCGCCGACTGCGGGACGATCCTCCTGCAAGATAAACAGCGGAAAA TCTACTGTGTGGCTTGTCAGGAGCTCGACTCAGACGTGGATAAAGATAACCCGGCACTGAATGCACAGGC GGCGCTCTCTCAAGCTCGGGAACACCAGCTGGCCTCATCTACAGAGCCTGCCTCGAGCTCTCGGCCCCCA TCCCAGCCACCAGTACCCCGTCCAGAGCACTGTGAAGGCGCAGCAGCGGGGCTCAAGGCAGCCCAGGCGC CTCCTCTTCCGGCTGCACCTCCAAATACGGACGCGGTGGCTTCCACACAGACGGCCCTCCTGCAGAAGCT AACCTGGGCGTCAGTCGAGCTGGGCTCTAGCACCTCCTTGGAGACTAGTATCCAGCTCTGTGGGCTTATC CGGGCTTGCGCCGAGGCCCTAGGCAGCCTGAAACAGCTGGATCACTGAGACCTCTATCCTGATCCCTGTT CCCCTGAGAAAAACCCTCCAGAAAAACAGTTTTTCCTCCGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5102521 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5102521 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10718 same embryo
	EMAGE:10719 same embryo
	EMAGE:10717 same embryo
	EMAGE:10720 same embryo
	EMAGE:10716 same embryo
	EurExpress:euxassay_001279 same experiment
	MGI:4828461 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source