EMAGE mouse gene expression spatial database

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EMAGE:10723

Wasf3 WAS protein family, member 3 ( MGI:2658986)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001282. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001282_01	euxassay_001282_02	euxassay_001282_03	euxassay_001282_04

euxassay_001282_05	euxassay_001282_06	euxassay_001282_07	euxassay_001282_08	euxassay_001282_09

euxassay_001282_10	euxassay_001282_11	euxassay_001282_12	euxassay_001282_13	euxassay_001282_14

euxassay_001282_15	euxassay_001282_16	euxassay_001282_17	euxassay_001282_18	euxassay_001282_19

euxassay_001282_20	euxassay_001282_21	euxassay_001282_22	euxassay_001282_23	euxassay_001282_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10723_wholemount_strong.wlz

10723_wholemount_moderate.wlz

10723_wholemount_weak.wlz

10723_wholemount_possible.wlz

10723_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10723_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 09 10 11 18 19 20

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22 23 24 weak expression: see section 06 07 08 20

telencephalon marginal layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10

olfactory cortex marginal layer

weak

regional

weak expression: see section 11 12 13 16 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 19

spinal cord ventricular layer

moderate

regional

moderate expression: see section 13 14 15

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 17

cervical ganglion

weak

regional

weak expression: see section 10 18

thoracic ganglion

weak

regional

weak expression: see section 13 14 15

neural retina

weak

regional

weak expression: see section 01 02 03 04 24

tail dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 11 12 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5371
Entity Detected:	Wasf3, WAS protein family, member 3 ( MGI:2658986)
Sequence:	sense strand is shown >T5371 GCCCTGTGTTACCAGGGGCCTCAAGATCCAGGTGAAGGGTAAGCTACAGGTGGGGATCCGAGGGGTCTGC AGATAGAGGAGGTACCCAGGCCCCTTGGTTACCCACCTTGGAGGAGGAGGCCTGAGGATGCGCCGCCTTC TCCGCAACGCCCCGCCCCCGCAGCGCCTACGCTGCCCTCTCCCGTTGGCTCTCCATTGGCCGCAGAGCTC GCCAATCCAGCCCCCTGGCCGTGAAGGGGGCGTGGCCGCGCGGTGCCGGGCCGGGGGCCGCGTCTGTCGC CGCTGCGCGCTGCGGTGCGGGCGGCAGGACGATGAAGCCGGGAGCGCGGCGCGCTCTGATCCCGCTTGCT GTTCCTACTGACACCTGAGCCGCGGTGGCGATGCTGCTGAGGCCCTTCCAGCCCACGAGTCAACCTACTT GATTGTCAGCCATGCCTTTAGTGAAGAGAAACATCGAACCCCGGCACCTGTGCCGGGGCGCCCTGCCGGA AGGAGTCACCAGTGAGCTGGAATGCGTGACCAACAGCACGCTCGCTGCAATCATCCGCCAGCTGAGCAGT CTGAGTAAACATGCAGAAGACATATTTGGTGAGTTATTTAATGAGGCTAACAACTTCTACATCAGAGCAA ATTCCCTTCAAGACAGAATCGATCGGCTTGCTGTCAAAGTCACCCAGCTGGACTCCACGGTGGAGGAGGT CTCACTACAGGATATCAACATGAAGAAAGCATTCAAAAGCTCTACCATCCAAGACCAGCAGGTGGTCTCC AAGAACAGCATTCCCAACCCTGTTGCTGACATTTATAATCAGAGTGACAAACCCCCGCCGCTGAGCATTC TGACACCGTACAGAGATGACAAGAAGGATGGGCTGAAGTTCTACACGGATCCCTCTTACTTCTTTGACCT CTGGAAAGAAAAGATGCTGCAGGACACAGAAGACAAGAGGAAAGAGAAACGACGTCAGAAGGAACAAAAG CGTGTAGATGGCACCACCCGAGAGGTGAAAAAGGTTAGAAAAGCCAGAAACAGGCGCCAGGAGTGGAATA TGATGGCGTATGACAAGGAGCTTAGGCCCGACAACAGGTTGTCTCAGAGTGTGCACCACGGAGCATCCTC CGAGGGATCTCTGTCCCCAGATACTAGGTCTCACACATCCGATGTCACCGATTATTCTTATCCTGCTACT CCCAACCATGCTTTGCAAGCACAGCCGGCGACACCCTCCTACACAGCTGGCGATGCACCACTGCATGGGA CCACAAACCAAGGCGCTGAGCACGAGTACCGACCGTCGTCTGCCTCGGCAAGGCACATGGCCCTGAACAG ACCTCAGCAGCCACCGCCACCGCCTCCTCCCCAGGCGCCGGAGGGGTCCCAGGCCTCTACGTCAGTGGCT CCAGCCGACTATGGGATGCTTCCAGCACAGATAATTGAATACTACAGCCCCTCAGGACCGCCACCACCTC CCCCTCCACCCATGATTCCCTCAGCACAAACTGCCTTCGTCAGCCCTCTCCAGATGCCCACACAGCCCCC GTTCCCTGCCTCAGCTGTCTCCACATACCCCACTCCTCCTCACCAGCCCTCCACTGGGCTCCTGGCCACA GCACCACCACCCCCAGGTCCCCCACCTCCTCCACCTGGTCCTCCTGGCCCCTCCTCCCTATCATCGTCCC CAATGCATGGTCCCCCTGTGGCTGAGGCAAAGCGCCCAGAGCCTGCACAGCCACCCATAAGTGATGCGAG AAGTGACCTCTTGGCTGCCATCCGGATGGGAATCCAACTGAAAAAGGTGCAGGAGCAACGCGAGCAGGAA GCCAAGCGGGAGCCTGTCGGGAATGACGTGGCCACCATCTTGTCTAGACGCATTGCTGTGGAGTACAGCG ACTCAGACGACGACTCTGAGTTTGATGAGAATGACTGGTCTGACTGAGGCACAAGGGCCAGGCGACCAGT GGACAGCCAGGGCCCCATATGCAAGTGTGTTAGGAGCTCCTGTGCTCTCTGCACCCTCACAGTGGTATTT CCCTGTAGCTTTAGTCCCTTTTGTATTAACAGTGTGATTTGCTTCTGCACATCCTTCTGGATTTGTTGAG TATTTACTGTGTAATACTTAAGTGCCACAGAACACAGCACACCGTGCTGCACACCAGGAAACTCGCAGTA TTAGTGCATCACCCTGGAACAGCATCTTGCTCCCCTCTCAGCCGTGAGAGTCCCCTCTCCTGTCCATCCT GATTTGGTGGGCTTGCCACGTATCTGCGCAGCTGCCCCACGTCAGCTCTCTGCAAGATCACTGGCTGTGT GTCTCAGTGATGTCTTCCAACCAGTGGGTGATACGTTTTCCTGTTTCTTCCCTTTGCAAATTGAATTCCA GGTAGATATGGTTGTAGAGGGGAAAGTTTAACAAATCAGAATGAAATGTTTGTCTGTTTGCACACTGGGC CTGGTGGCCTCCAAGGAAATGTGTACGATCGTTTGCTTTCTCTATCTTGGCGCTTGCAACCGAGAGCGGC CATTTTTATGAGGGGAGCCCTGTGGGTACTCATGTGGAAAGTGCCCACTGACTTTGGCAGATGGAGTTCA CTTTCATGGCTAGCCCTGATAGCGTTGGGCACCAGTTAGCCAGGCCATGTTCAAGCTGCCTAGAGTCTGC AGAGCTTGCATGGCAGAGCGGCCGC
Notes:	The probe template was PCR amplified from IMAGE:5363785 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363785 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10725 same embryo
	EMAGE:10724 same embryo
	EMAGE:10721 same embryo
	EMAGE:10722 same embryo
	EurExpress:euxassay_001282 same experiment
	MGI:4829188 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source