EMAGE mouse gene expression spatial database

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EMAGE:10725

Acaa1b acetyl-Coenzyme A acyltransferase 1B ( MGI:3605455)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001231. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001231_01	euxassay_001231_02	euxassay_001231_03	euxassay_001231_04

euxassay_001231_05	euxassay_001231_06	euxassay_001231_07	euxassay_001231_08	euxassay_001231_09

euxassay_001231_10	euxassay_001231_11	euxassay_001231_12	euxassay_001231_13	euxassay_001231_14

euxassay_001231_15	euxassay_001231_16	euxassay_001231_17	euxassay_001231_18	euxassay_001231_19

euxassay_001231_20	euxassay_001231_21	euxassay_001231_22	euxassay_001231_23	euxassay_001231_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10725_wholemount_strong.wlz

10725_wholemount_moderate.wlz

10725_wholemount_weak.wlz

10725_wholemount_possible.wlz

10725_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10725_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 14 15 16 17

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 18 19 20 21

pancreas

weak

regional

weak expression: see section 10 11 12 13 18 19

metencephalon basal plate

moderate

regional

moderate expression: see section 09 10 11 17 19 20 weak expression: see section 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 09 20

trigeminal v ganglion

weak

regional

weak expression: see section 05 06 07 08 09 10 19 20 21 22 23 24

vagus x ganglion

weak

regional

weak expression: see section 10 19

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 17 18 19

anal canal

weak

regional

weak expression: see section 15

lower jaw incisor

strong

regional

strong expression: see section 12 13 18 moderate expression: see section 17

upper jaw incisor

strong

regional

strong expression: see section 12 13 14 18 moderate expression: see section 17

kidney calyx

weak

regional

weak expression: see section 09 10 11 12 19 20 21 22

chondrocranium

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4922
Entity Detected:	Acaa1b, acetyl-Coenzyme A acyltransferase 1B ( MGI:3605455)
Sequence:	sense strand is shown >T4922 CCCACCTCCGCGATGCATCGGCTGCAGGTAGTGCTGGGTCACCTGGCCGGCCGGCCCGAGTCGAGCTCCG CGCTGCAAGCTGCGCCCTGCTCCGCTGGCTTCCTGCAGGCCTCGGCCTCCGACGTGGTGGTGGTGCACGG ACGGCGCACTCCCATTGGCCGCGCCAGCCGTGGCTGCTTCAAGGACACCACCCCCGACGAGCTTCTGTCG GCCGTGTTGACCGCGGTTCTCCAGGACGTGAAGCTAAAGCCTGAGCAGCTGGGAGACATCTCCGTGGGCA ATGTGCTTCAGCCCGGGGCTGGAGCCATTATGGCACGCATCGCCCAGTTCCTGAGCGGCATCCCAGAGAC TGTACCTTTGTCTACGGTCAACAGACAGTGTTCATCGGGACTGCAGGCAGTGGCCAACATTGCTGGTGGC ATCAGAAATGGGTCTTATGACATTGGCATGGCCTGTGGGGTGGAGTCCATGACCCTGTCCCAGAGAGGGA ACCATGGGAATATTTCTTCCCGCCTGCTGGAGAACGAGAAGGCCAGAGACTGCCTGATTCCTATGGGGAT AACTTCGGAGAATGTGGCTGAGCGGTTTGGCGTTTCACGGCAGAAGCAGGATGCCTTTGCGCTGGCCTCC CAGCAGAAGGCAGCAAGCGCCCAGAGCAGAGGATGCTTCCATGCTGAGATTGTGCCTGTGACAACCACTG TCCTGAATGACAAGGGTGACAAGAAAACCATCACTGTGTCTCAGGATGAGGGTGTCCGCCCCAGCACCAC CATGCAGGGCCTGGCCAAGCTGAAGCCTGCCTTCAAGGATGGAGGCTCTACCACGGCTGGAAACTCCAGT CAGGTGAGTGATGGAGCAGCTGCCGTCCTGCTGGCTCGGAGGTCCAAGGCTGAAGAACTGGGCCTCCCCA TCCTTGGGGTCCTGAGGTCCTATGCAGTGGTCGGGGTCCCTCCTGACGTCATGGGCATCGGACCTGCCTA TGCCATCCCTGCAGCCTTGCAGAAAGCAGGGCTGACTGTGAATGACATAGACATCTTTGAGATCAATGAG GCCTTTGCAAGTCAGGCCGTCTACTGTGTGGAGAAGTTAGGAATTCCTGCAGAGAAGGTGAACCCGCTGG GGGGTGCAATAGCCCTGGGCCACCCCCTGGGCTGCACGGGAGCAAGGCAGGTTGTCACGCTACTCAATGA ACTGAAGCGCCGTGGCAGACGGGCTTACGGCGTGGTATCCATGTGCATCGGGACTGGGATGGGAGCTGCT GCGGTCTTTGAATACCCTGGGAACTGAGGCCTGGCTGCAGGCGGCACAACCCAGAGAGTGCCACAGTGGT GTCCAGAGAGGGACGCTACAGAAGCCGTCTGCGTGGGACACTCAGCAGTGGAGGGGTGTGTCACAGCACT TTAATTTAGAAAATGTAATTGCAGCATGGGTACACGTGGGCACTGGGGCATCCAATCCGGTTCTCTCGGT CGAACCAAAGACAGTGCATGCTATGCTTGACCACAGGACAACATTCATTTCTGTGGGACGGTGCCTTCAT GAATGGTGGATGTGATGGGCACAGTAAACGTGGGTGAACTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5123887 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5123887 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10724 same embryo
	EMAGE:10723 same embryo
	EMAGE:10721 same embryo
	EMAGE:10722 same embryo
	EurExpress:euxassay_001231 same experiment
	MGI:4822894 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source