EMAGE mouse gene expression spatial database

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EMAGE:10794

Gem GTP binding protein (gene overexpressed in skeletal muscle) ( MGI:99844)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003192. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003192_01	euxassay_003192_02	euxassay_003192_03	euxassay_003192_04

euxassay_003192_05	euxassay_003192_06	euxassay_003192_07	euxassay_003192_08	euxassay_003192_09

euxassay_003192_10	euxassay_003192_11	euxassay_003192_12	euxassay_003192_13	euxassay_003192_14

euxassay_003192_15	euxassay_003192_16	euxassay_003192_17	euxassay_003192_18	euxassay_003192_19

euxassay_003192_20	euxassay_003192_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10794_wholemount_strong.wlz

10794_wholemount_moderate.wlz

10794_wholemount_weak.wlz

10794_wholemount_possible.wlz

10794_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10794_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 08 09 10 17 18 19

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 18 19 20 weak expression: see section 03

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 14 15 weak expression: see section 08 09 10

telencephalon ventricular layer

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 weak expression: see section 01 02 03 04 05 06 07 08 09 10

dorsal grey horn

strong

regional

strong expression: see section 15 16 moderate expression: see section 13 14 weak expression: see section 12

esophagus

strong

regional

strong expression: see section 11 12 13

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15

lower jaw molar

strong

regional

strong expression: see section 19 moderate expression: see section 18

upper jaw incisor

moderate

regional

moderate expression: see section 09 10 11 14 15

upper jaw molar

strong

regional

strong expression: see section 19 moderate expression: see section 18

testis

strong

regional

strong expression: see section 06 07 08 20 21

clavicle

strong

regional

strong expression: see section 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5434
Entity Detected:	Gem, GTP binding protein (gene overexpressed in skeletal muscle) ( MGI:99844)
Sequence:	sense strand is shown >T5434 CACACAGCCTCGGACTGCGGAGCCCGCATCGCAGACGTCAGGCGTTGCAGCAGGCGGATCGACGGCAGAC GCCCACCCAGCGCCCAGTGCAGCCAAGATCCCGCAATGACTCTGAATAATGTCACCATGCGCCAAGGCAC TGTGGGCATGCAGCCACAGCAGCGCTGGAGTATCCCTGCTGATGCCAGGCATCTGATGGTCCAGAAGGAT CCCCACCCCTGCAACCTCCGAAACCGCCACTCTACTGCTCCGGAAGAGCACTGCCGGCGGAGCTGGTCGT CCGACTCCACAGACTCGGTCATCTCTTCTGAGTCTGGAAACACCTACTACCGAGTAGTGCTTATAGGGGA GCAAGGAGTGGGCAAGTCCACCCTGGCCAACATCTTTGCAGGTGTGCATGACAGCATGGACAGCGACTGT GAGGTCTTGGGAGAAGATACATATGAGCGTACCCTGGTCGTTGACGGAGAGAGTGCAACCATTATCCTCC TGGACATGTGGGAAAATAAGGGGGAGAACGAATGGCTCCATGACCACTGCATGCAGGTCGGGGATGCCTA TCTGATCGTCTACTCTATCACAGACCGTGCAAGCTTCGAGAAGGCATCTGAGCTGAGGATTCAGCTCCGC AGGGCCCGGCAGACAGAAGACATTCCTATAATTTTGGTTGGCAACAAAAGCGACTTAGTGCGGTGTCGAG AAGTGTCTGTGTCAGAAGGGAGAGCTTGTGCTGTGGTGTTCGACTGCAAATTCATCGAGACCTCTGCGGC TGTGCAGCACAACGTGAAGGAACTGTTTGAGGGCATTGTGCGACAGGTGCGCCTGCGCAGGGACAGCAAG GAGAAGAATGAGAGGAGGCTGGCCTACCAGAAGAGGCGGGAGAGCATTCCCAGGAAAGCCAGGCGCTTCT GGGGCAAAATTGTGGCCAAAAACAACAAGAACATGGCTTTCAAGCTCAAGTCAAAATCCTGCCATGACCT GTCTGTGCTCTAGGCACCCAGTGTCACCCAGATGTCCCCTTGGTGGACATCGTTGAAGGCTATTGGGACC AGTGATCTATATTAGATTGGATACATAAGCATTGTTAGACGCAACTTCCCCTATGGCAGATGGAAACCAA CAGGTTAGCCTTGTGGGCAACCAAGTGCACGGGCAATGAATGAGCTCTGTCAAGAGTCAGTATTTATTCA TAGGAAAAGCTTGACCTGCTACGTGGATGTCTCAGACTCATTTAAGACACGCTTCGGGTTCACATGAGTT TCTCTCTCCTTTGGACAGCAGAATATTTTTTTCCTCAGTGTTGTTCCATGTGATTTCGAGGTCCTTGGGT CATATAGAAATGTAGGGAAATGGTGGTAGTTTATTGGAAGGAGAAGGGCTCACTGCATACTTATATCCCT GAAACGACATCTTTAGAGCTGGCCTCATCACAGTTGTGACTATTTCTGCTCCAGTGAAGAGAATTGTTAG AATTTGCTGGAAACTGAGGCTTACTAACAGTTTTTGTTTAAAGACCACAGAGATTTGTAGACTTAGGAGC TGATATGTGTACTACTTATAGGTTCAAAAAATTGTTTACTTATGTGTCGTAGAAGTGTTTATTTTGAGGA AACTATTTTTTTTTTTGCCAAATTCTACTTAGTCAAATCATCTTCTATGTCTTGCTGTTTTTTAAATCAT TAAGCTATCATAAAATATTTTTTTAAAAAATCTCAACTATATTGATAACCTGCAGTGCAAAATTTTAAAT ATAGTCCTGTTTTTCCCCCAAAACATAAACATGCCCCATCCTTTGGGTTGCTTCTTGTATGCCACAGCTG AATTATATTTATTATTTTGCAAATAACCATTTTAATATTTGATAAAGCATATTTATGAGCATATTTCTTA CTGAGAAAATGTCTGTTTTATTACCTTTTTATATTTTTCAAAGTATTCAAGTTTTTACCTATATGTCTTA TAATAAAATAAATAAAATCTTTGAAAAGGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5368352 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5368352 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10791 same embryo
	EMAGE:10793 same embryo
	EMAGE:10792 same embryo
	EurExpress:euxassay_003192 same experiment
	MGI:4825041 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source