EMAGE mouse gene expression spatial database

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EMAGE:10803

Osgin1 oxidative stress induced growth inhibitor 1 ( MGI:1919089)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003219. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003219_01	euxassay_003219_02	euxassay_003219_03	euxassay_003219_04

euxassay_003219_05	euxassay_003219_06	euxassay_003219_07	euxassay_003219_08	euxassay_003219_09

euxassay_003219_10	euxassay_003219_11	euxassay_003219_12	euxassay_003219_13	euxassay_003219_14

euxassay_003219_15	euxassay_003219_16	euxassay_003219_17	euxassay_003219_18	euxassay_003219_19

euxassay_003219_20	euxassay_003219_21	euxassay_003219_22	euxassay_003219_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10803_wholemount_strong.wlz

10803_wholemount_moderate.wlz

10803_wholemount_weak.wlz

10803_wholemount_possible.wlz

10803_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10803_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

homogeneous

moderate expression: see section 05 06 07 08 11 12 15 16 weak expression: see section 09 10 13 14 17

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 04 08 12 20 weak expression: see section 01 02 05 06 07 09 10 11 13 14 15 16 18 19 21 22 23

telencephalon marginal layer

moderate

regional

moderate expression: see section 03 04 08 20 weak expression: see section 05 06 07 09 10 11 13 14 15 16 18 19 21 22

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 08 weak expression: see section 09 10 11 14 15 16

hindbrain

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 11 12 15 16 20 weak expression: see section 01 02 09 10 13 14 17 18 19

midbrain

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 11 12 15 16 weak expression: see section 09 10 13 14 17

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 20 weak expression: see section 02 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 20 weak expression: see section 01 02 05 06 07 08 16 17 18 19 21

vagus x ganglion

weak

regional

weak expression: see section 07 08 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 weak expression: see section 05 06 16 17 18 19

spinal cord

moderate

homogeneous

moderate expression: see section 07 08 11 12 15 weak expression: see section 09 10 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 15 16 weak expression: see section 06 09 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4889
Entity Detected:	Osgin1, oxidative stress induced growth inhibitor 1 ( MGI:1919089)
Sequence:	sense strand is shown >T4889 CCCAGGGCTAATGGGCATCCTGTTCTTGCAGCAGGGCACCGTGACAAAGTCTCTCACCGTGTGACCAAGT TCCTCTGAGTGTCCAGACAACCCAGGCTCACCAGCTCCCTCCAGCTATCTACCATCCATCAGGTCAGCTG CCAGCCCCAGGCTGAACACCCACCCACCGAGCTATGACCTCCTGGAGGCACGACTCCCTTGGGGCCAGCA GCTCAGAGCCCCTCCCAGTAGTCATCATCGGCAATGGCCCCTCCGGTATCTGCCTGTCCTACCTGCTCTC TGGACACATCCCCTACGTGAAACCAGGAGCCGTCCACCCACACCCCCTGCTGCAGAGAAAGCTGGCCGAG GCTCCAGGGGTCTCCATCCTGGACCAGGACCTAGAGTACCTTTCCGAAGGCCTCGAAGGCCGAAGCCAGA GTCCTGTGGCCCTGCTCTTTGATGCCCTCCTGCGTCCTGACACAGACTTTGGAGGCAGCATAGACTCTGT GCTCTCCTGGAAGCGGCAGAAGGACCGCGCTGTTCCCCACCTTGTCCTGGGACGGAACCTCCCCGGGGGC GCCTGGCATTCCATTGAAGGCTCCATGGTGACCTTGAGCCAAGGCCAGTGGATGAGTCTCCCAGACCTGC AGGTCAAAGACTGGATGCGGAAGAAATGCAGAGGTCTCCGCAACAGCAGAGCCACAGCCGGTGACATCGC CCACTACTACCGGGACTACGTGATCAAGAAAGGCCTAAGTCACAACTTTGTGTCTGGTGCCGTGGTCACT GCCGTGGAGTGGGCTAAGTCCGAGCATGGCAGTCCCGAGGTCCAGGCATCCAGTCCCCTCTTCCAAGTGA ATGGCTACTTGACTACCAAGGACCACGGCCACCAGCCCTTCTCACTGCGGGCCCGTAATGTGGTCCTGGC CACAGGCACCTTTGACAGCCCAGCCATGCTAGGCATCCCAGGAGAGACCCTGCCCTTTGTCCACCACGAC CTGTCAGCCCTGGAGGCAGCCCTTCGGGCAGGCACTGTGAACCCAACCTCAGACCCGGTGCTGATTGTGG GAGCAGGGCTATCTGCAGCCGATGCTGTCCTCTTTGCCCGTCACTACAACATCCAGGTGATCCATGCTTT CCGCCGGTCCGTGCATGACCCCGGCCTGGTATTCAACCAGCTGCCCAAGATGCTATACCCTGAGTACCAC AAAGTGCAGCAGATGATGCGCGATCAGTCCATCTTGTCTCCTAGCCCCTATGAGGGCTACCGCAGCCTCC CTGAGCACCAGCCACTGCTCTTCAAAGAGGACCACCAAGCAGTGTTCCAGGACCCACAGGGGGGCCAGCA GCTCTTTGGGGTCTCCATGGTGCTGGTCCTCATTGGCTCCCACCCCGACCTCTCCTACCTCCCCAGGGCA GGTGCTGACTTGGTCATAGACCCAGATCAGCCACTGAGTCCCAAGAGGAACCCCATTGACGTGGACCCCT TCACCCATGAGAGCACTCACCAGGAGGGCCTGTATGCCCTGGGGCCGTTGGCTGGGGACAACTTTGTGAG ATTTGTCCAGGGCGGGGCCCTGGCTGCTGCCAGCTCCCTGCTGAAGAAGGAGACCAGGAAGCCACCTTAA CATTAGCCTGGCACCCTGACACCCAGGCCCTGAAGAGGAAGAGGGCTTATCACAGCCCAGCGGGACGTGA GCCCATCTAAAGATTTCCCATTTGGGAACCAGTCTCCCCAGCAGAAGGGGAAGACTTGAGCCATGTGGAT GGACTATCTGCCCCAGAGGCATGGGGAACACAGGCTGCCCTACCCTCAGGCGAGATGAGGCCCCAGGTGG GAGCAGTAGCCATAGACCAGGGGGCCCCCTTGAAACTGTCAGTGTGTGCTGGAGTCACAGGGCCAGCTGA GTGTCCAACTTGGAGGGCCCCAGGCCCAACTTTTCACCATCAGATCTGTAATAAAACCAGCTGTGCCTCC AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5099665 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5099665 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10804 same embryo
	EMAGE:10802 same embryo
	EMAGE:10801 same embryo
	EMAGE:10805 same embryo
	EurExpress:euxassay_003219 same experiment
	MGI:4826995 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source