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EMAGE:10864

Pip4k2a phosphatidylinositol-5-phosphate 4-kinase, type II, alpha ( MGI:1298206)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864
"Pseudo-wholemount" of euxassay_003374. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003374_01 euxassay_003374_02 euxassay_003374_03 euxassay_003374_04
EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864
euxassay_003374_05 euxassay_003374_06 euxassay_003374_07 euxassay_003374_08 euxassay_003374_09
EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864
euxassay_003374_10 euxassay_003374_11 euxassay_003374_12 euxassay_003374_13 euxassay_003374_14
EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864
euxassay_003374_15 euxassay_003374_16 euxassay_003374_17 euxassay_003374_18 euxassay_003374_19
EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864 EMAGE:10864
euxassay_003374_20 euxassay_003374_21 euxassay_003374_22 euxassay_003374_23 euxassay_003374_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10864Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10864_wholemount_strong.wlz
10864_wholemount_moderate.wlz
10864_wholemount_weak.wlz
10864_wholemount_possible.wlz
10864_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10864_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 22 23
head mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 22 23 24
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 10 11 12 13 14 15
foregut-midgut junction
weak weak
regionalweak expression: see section 12 13
medulla oblongata basal plate
moderate moderate
regionalmoderate expression: see section 07 08 14 15
metencephalon basal plate
strong strong
regionalstrong expression: see section 06 16 moderate expression: see section 17
pons mantle layer
strong strong
regionalstrong expression: see section 06 moderate expression: see section 05 16
ventral grey horn
moderate moderate
regionalmoderate expression: see section 09 15 16 17 weak expression: see section 10 12
dorsal root ganglion
weak weak
regionalweak expression: see section 12 13 15 16 17 18 19
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 12 13 15 16
tongue mesenchyme
weak weak
regionalweak expression: see section 11 12 13 14 15 16 17
stomach
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 not examined expression: see section 12 13 14 15
hindgut
weak weak
regionalweak expression: see section 13 14 15 16 17 18
rectum
weak weak
regionalweak expression: see section 14 15
midgut
weak weak
regionalweak expression: see section 11 14 15 16 17 18 19 20
midgut loop
weak weak
regionalweak expression: see section 09 12 13
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 16 17
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 17 weak expression: see section 18
liver lobe
moderate moderate
regionalmoderate expression: see section 01 02 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 03 04
renal medullary interstitium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 17 18 19 20 21
testis
weak weak
regionalweak expression: see section 06 07 08 20 21
midgut loop lumen
weak weak
regionalweak expression: see section 10 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6750
Entity Detected:Pip4k2a, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha ( MGI:1298206)
Sequence:sense strand is shown

>T6750
CCGGGCTGGCGTGTAGGGAGGGCGGGTCCCCGGCCTCGGCGGCGCGGCGGTGGAGGGGACCGAGACTGCG
ACCATGGCGACCCCTGGCAACCTGGGGTCCTCCGTCCTGGCGAGCAAGACCAAGACGAAGAAGAAGCACT
TCGTGGCTCAGAAAGTGAAGCTGTTCCGGGCCAGCGACCCGCTGCTCAGCGTGCTCATGTGGGGGGTCAA
CCATTCGATCAATGAACTGAGCCACGTTCAAATCCCTGTCATGTTGATGCCTGATGACTTCAAAGCCTAC
TCAAAGATAAAGGTTGACAACCACCTTTTTAACAAGGAAAACATGCCGAGCCATTTCAAGTTTAAGGAAT
ACTGCCCAATGGTCTTCCGGAATCTGCGGGAGAGGTTTGGAATCGACGACCAAGATTTCCAGAATTCCTT
GACCAGAAGTGCACCCCTTCCCAATGACTCCCAGGCTCGCAGCGGGGCTCGGTTTCACACGTCTTATGAT
AAAAGATACGTCATCAAGACCATTACCAGTGAGGACGTGGCAGAGATGCACAACATCCTGAAGAAGTACC
ACCAGTATATAGTGGAATGTCATGGGGTCACACTTCTTCCTCAGTTCTTGGGAATGTACCGGCTTAATGT
CGATGGAGTGGAAATATATGTGATTGTTACAAGGAATGTGTTCAGCCACCGGCTATCTGTATATAGGAAA
TACGACTTAAAGGGCTCGACAGTGGCTAGAGAAGCTAGTGATAAAGAAAAGGCCAAAGAGCTGCCAACTT
TAAAGGATAATGACTTCATCAATGAGGGCCAAAAGATCTATATTGATGACAACAACAAAAAGATATTCCT
AGAAAAACTGAAAAAGGATGTCGAGTTCCTTGCACAGTTAAAACTCATGGACTACAGCCTCCTGGTGGGG
ATTCACGATGTGGAGCGAGCAGAGCAGGAGGAGGTGGAGTGTGAGGAGAACGATGGGGAAGAAGAAGGAG
AGAGTGACAGCACCCACCCGATCGGGACGCCACCAGACAGTCCCGGAAATACACTCAACAGCTCACCACC
CTTGGCTCCTGGAGAGTTTGATCCAAACATTGATGTTTATGCAATTAAATGCCATGAGAATGCGCCTAGA
AAAGAGGTATACTTCATGGCGATTATTGACATCCTTACTCATTACGATGCAAAAAAGAAAGCTGCCCACG
CTGCAAAAACTGTTAAACATGGCGCTGGAGCTGAGATCTCGACTGTGAACCCAGAGCAGTATTCCAAGCG
CTTTTTGGACTTTATTGGGCACATCTTGTAACCTTCTGCACAGCCCCGGATGGGCATGAACTCGGGAAGG
CTGGTGGTGGCTTCAGGGTAGGAAGAAAAGGAAAACCGAACCCCAGGAAGCGCTCACCTTGCAGGAAGCA
AGCCTTGTTTACATCTTCAAGCTGACATGACTGAAGTTGGGGGCTACTCGCTTTAACAGCTACCTGATAT
TTCCCAGCATCGCTATTTCTGACTCTGTACGTGTGCACACACGTGCGTGCACACACTCTCGCGCACATTT
TTTTAAATAATTAATTAATTAATTAATTAAAACTGATCAGCTTGAATCAGAGCTTGTAACAGCCAGACCC
CTCTGATTCCAGCTGTGGCCCCACGGATAGATGAAGAGATGAGCCCGTGGGGGAAGGGAACGGAAATGCA
CCTCAATGCAGCACCACAAGAGAAACTGTAGATCAGTTTGTTTTCTGACTTGAAAGATATGAGATTGTTC
ATCAGGGTGGTGGTGGTGGTGGTGATGATGATGATCATGCTCATAATGATGGTGATTAAAAGAAAGAAAA
TTTTATATCAAATGTTACACTTCTGGCCCACAGAAAGACTACTCTGAATAGTGGTTACCTAAGCCCACAG
CCCTGGTAAAGGGATACAAGCATCTCCCTGTGTACTGGTTCAGGGTTCCCTCCTAAACTGTCAACATCCC
CAGATGTCCCAGGCTAAGCCCTTCATCTTCCAAGAGTCTGATGCCAAGAACCTGTCAACAATTTCACCTC
CAGGAAAACCACTTGCATTTCTCTTCTCGCCTGGAGCCCATCACTGTTACCATCCTTAAGTTGCACTGCA
GAAAGTCACTCTGCAAAACAGATGTAAAACGTCCCTGAAAGGTGCATAGTTTGGGGCAAATGCGGCTACA
TCTCCCAATGCAGGAAGATGAGCAGGGAGTTCAAACCCTCCAGTGGCCGAAGGCAAACCCAGTTATGGTT
TATGTTTAGCCATTGTTTAGGAATGGAATTTTGTTATGAATTATCCCTTTTAACTGAAACCCTCAGTTTT
ACTGTTTACATGGTTAGAGAAACAGGGATATTTTTGAATCTAAAAATTTAATATACAGCATGTGATTTTT
GAAGTTTACATGTAAAGCCATTTTGCAGTGTAGGGAACATAATGTTTGTTGTGTTTTGAGATATGTCTTG
CAGTTGTGTTTCTGAGTGAGTGTTTTGTTGAGAGTACTTAGTAGACAGTCTATCACATAAAAGCAAAAGG
ATTTTGTTCCTCAAGATGTGTATTTATCAATCTAGTGGGGTTTTTTTTCTATAAAAAAATTAAACTCAAA
ATCTGGTTATGTAAGTTCATTGCCCTAAACTGTGCTGATCGTGTGTAACTGAGATTAAAAAACAAAACAA
AACAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3672732 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3672732 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10868 same embryo
 EMAGE:10867 same embryo
 EMAGE:10865 same embryo
 EMAGE:10866 same embryo
 EurExpress:euxassay_003374 same experiment
 MGI:4827214 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS