EMAGE mouse gene expression spatial database

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EMAGE:10865

Bub1 budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) ( MGI:1100510)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003361. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003361_01	euxassay_003361_02	euxassay_003361_03	euxassay_003361_04

euxassay_003361_05	euxassay_003361_06	euxassay_003361_07	euxassay_003361_08	euxassay_003361_09

euxassay_003361_10	euxassay_003361_11	euxassay_003361_12	euxassay_003361_13	euxassay_003361_14

euxassay_003361_15	euxassay_003361_16	euxassay_003361_17	euxassay_003361_18	euxassay_003361_19

euxassay_003361_20	euxassay_003361_21	euxassay_003361_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10865_wholemount_strong.wlz

10865_wholemount_moderate.wlz

10865_wholemount_weak.wlz

10865_wholemount_possible.wlz

10865_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10865_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22

adrenal gland

moderate

regional

moderate expression: see section 08 09 10 17 18 19

thymus primordium

strong

regional

strong expression: see section 10 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 10 16 17 18 19

foregut-midgut junction

moderate

regional

moderate expression: see section 08

pancreas

strong

regional

strong expression: see section 05 06 07 08 09 10 20 21 moderate expression: see section 11 15

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 09 10 21 22

3rd ventricle

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

4th ventricle

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

cochlea

moderate

regional

moderate expression: see section 02 15 16

cochlear duct

moderate

regional

moderate expression: see section 01 17 18

retina

moderate

regional

moderate expression: see section 01 02 03 04 22 not examined expression: see section 20 21

naris

strong

regional

strong expression: see section 12 moderate expression: see section 13 14 16 17 18 19 weak expression: see section 15

viscerocranium

moderate

regional

Expression in the turbinate bone.

tongue

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

midgut

moderate

regional

moderate expression: see section 12 13 14 15 16

midgut loop

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

lower jaw incisor

strong

regional

strong expression: see section 11 12 16 moderate expression: see section 13 15 not examined expression: see section 17 19

lower jaw molar

moderate

regional

moderate expression: see section 06 07 08 18 19

upper jaw incisor

strong

regional

strong expression: see section 12 16 moderate expression: see section 13 not examined expression: see section 17 19

upper jaw molar

moderate

regional

moderate expression: see section 06 07 08 18 19

not examined

regional

not examined expression: see section 22

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

metanephros

moderate

regional

moderate expression: see section 20

renal cortex

strong

regional

strong expression: see section 07 08 09 10 11 16 17 18 19 moderate expression: see section 20

genital tubercle of male

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

testis

moderate

regional

moderate expression: see section 05 06 07 08 20 21

lung

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6744
Entity Detected:	Bub1, budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) ( MGI:1100510)
Sequence:	sense strand is shown >T6744 CGAGACTCGGGGTTAGGGGCTGATGGGGAGTTTGGTCGGCGGCTGGTGGGGTATTTCGCCGCTGCGCGTT TTTTCTCGTCATGGACAACCTAGAAAATGTCTTTCGCATGTTTGAAGCCCATATGCAAAGCTACACGGGT AATGACCCACTTGGAGAATGGGAAAGCTTTATAAAGTGGGTAGAAGAGAATTTTCCTGACAATAAAGAAT ACTTGATGACATTATTAGAACATTTAATGAAGGAATTTTTACATAAGAAGAACTACCACAATGATTCAAG ATTCATCAATTATTGCTTAAAATTTGCTGAGTACAACAGCGACCGTCATCAGTTTTTTGAGTTTCTGTAC AACCAGGGAATTGGAACCAAGTCATCATATATATACATGTCCTGGGCAGGGCATCTGGAAGCCCAGGGAG AGCTGCAGCATGCCAGTGCTATTTTTCAGACAGGAATTCACAATGAGGCTGAACCTAAAGAACTACTACA GCAACAATACAGGCTATTCCAAGCACGCCTTACTGGAATCCATTTGCCAGCTCAAGCTACAACCTCAGAA CCTTTGCATAGTGCACAGATTTTAAACCAAGTTATGATGACAAACTCAAGTCCAGAAAAAAACTCAGCCT GTGTTCCTAAGAGTCAGGGTTCAGAATGTTCTGGTGTGGCATCTTCCACTTGTGATGAAAAGTCTAATAT TAGGGAACAAAGGGTGATCATGATTTCCAAGTCAGAATGCTCTGTCAGCTCATCTGTGGCACCCAAGCCT GAGGCTCAGCAAGTTATGTACTGCAAGGAAAAGCTTATTCGTGGAGATTCAGAATTTTCTTTTGAAGAAC TGAGAGCCCAGAAATATAATCAAAGGAAGAAGCATGAGCAGTGGGTTAGTGAAGACAGAAATTATATGAA AAGGAAAGAAGCAAATGCTTTTGAAGAGCAATTATTAAAACAGAAAATGGATGAACTTCACAAGAAATTG CATCAAGTGGTGGAATTGTCACACAAGGACCTTCCTGCTTCTGAGAACAGGCCTGATGTTAGTCTAGTAT GTGTTGGACAAAATACTTGCTCCCAGCAGGAATTGAGGGGTCCAAGTCTTTCATCCATCAGTCATCAGAC CTCAGAGAGTTCAGGAGAGAAACCACAGGAAGAACCTTCTGTTCCTCTTATGGTAAATGCTGTTAACAGC ACTTTGCTGTTCCCAGCTGCCAACCTGCCAGCTCTTCCTGTTCCTGTAAGTGGCCAGTCATTGACAGACT CCAGATGTGTGAATCAAAGTGTTCATGAATTCATGCCACAGTGTGGACCAGAAACAAAAGAAGTGTGTGA AACAAATAAAGTTGCCAGCATTAATGATTTTCATACAACTCCAAACACATCATTGGGAATGGTTCAAGGA ACACCATGCAAAGTGCAGCCATCACCAACTGTCCACACCAAGGAAGCATTAGGTTTCATCATGGACATGT TTCAGGCTCCAACACTTCCTGACATTTCTGATGATAAAGATGAATGGCCATCTCTGGACCAAAATGAAGA TGCATTTGAAGCCCAGTTTCAAAAAAATGCAGTATCTTCGGGAGATTGGGGAGTTAAAAAAATTATGACT TTGTCATCTGCTTTTCCTATTTTTGAAGATGGAAACAAAGAAAATTATGGCTTACCACAGCCTAAAAATA AGCCCTTAGGAGCTAGGACCTTTGGAGAACGATCTCTCAGTAAATATTCCTCGAGATCAAATGAAATGCC TCACACTGATGAGTTTATGGATGATTCAACAGTATGTGGTATTCGCTGCAACAAAACTCTAGCTCCCAGT CCTAAAAGTATAGGAGACTTTACATCTGCTGCCCAACTTTCGTCTACACCATTCCACAAATTTCCAGCAG ATTTAGTACAGATTCCAGAAGATAAAGAAAATGTGGTAGCCACACAGTATACACATATGGCTTTGGATTC TTGTAAAGAAAACATAGTGGACCTCTCAAAAGGCAGAAAGCTTGGGCCAATTCAAGAGAAAATTTCAGCA TCTTTACCCTGTCCTAGTCAGCCTGCCACAGGTGGTTTGTTCACCCAGGAAGCAGTGTTCGGCCTTGAGG CTTTTAAATGCACAGGCATTGACCATGCGACAGTGGAAGACCTATCCGATGCCAATGCTGGGCTCCAAGT TGAATGCGTGCAGACACTTGGAAATGTCAATGCTCCAAGCTTTACTGTTGAGAACCCATGGGATGATGAA TTGATTCTTAAACTTCTCTCTGGACTTTCTAAGCCAGTTACTTCCTATTCAAATACTTTTGAGTGGCAGA GTAAACTTCCAGCCATCAAGACCAAGACAGAATATCAATTGGGTTCTTTGCTGGTCTATGTGAATCACCT TCTTGGAGAAGGAGCCTTTGCTCAAGTCTTTGAAGCTATTCATGGAGATGTGAGAAATGCCAAAAGTGAA CAGAAATGCATTTTGAAGGTGCAGAGACCTGCCAACTCCTGGGAATTCTACATTGGGATGCAGCTGATGG AAAGACTAAAGCCAGAAGTACATCACATGTTCATCAAGTTTTATTCTGCTCATTTATTCAAGAACGGCAG CATATTAGTAGGGGAACTCTACAGCTATGGGACGTTACTAAATGTCATTAACCTCTATAAAAATACCTCT GAAAAAGTGATGCCCCAGGCTCTTGTCCTCACTTTCGCTATCAGAATGCTTTACATGGTTGAACAAGTCC ACAGCTGCGAAATCATTCATGGAGACATTAAGCCAGATAACTTCATACTAGGACACAGATTTTTGGAACA GGCTGATGAAGACTTAGCTACCGGCTTGGCATTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTC CCTAAAGGAACTGTATTTACAGGAAAATGTGAAACATCTGGTTTTCAGTGTCCTGAGATGCTCAGTAACA AGCCATGGAACTACCAGATTGATTACTTTGGAGTTGCTGCAACAATATACTGTATGCTCTTTGGCTCTTA CATGAAAGTAAAAAATGAAGGAGGAGTCTGGAAACCTGAAGGTCTTTTTAGAAGGCTTCCTCATTTGGAT ATGTGGGAGGAATTTTTTCACATCATGTTGAATATACCGGATTGTCATAATCTTCCATCTTTGGATTTTC TGAGACAGAATATGAAGAAATTACTTGAACAACAGTATTCCAACAAGATTAAGACCTTGCGTAATAGGCT AATTGTGATGCTTTCAGAATATAAGCGTTCAAGAAAATAAAATGAATATGGACCTTCTCCAAAACACACT TAGTAAATTTAAACTCTCACTTTGAATCCTATTTTTTTCATACTTACTATTTATGTATGTTTAAAAAAAA TAAAGCCCATATGATATACCCAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3671932 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3671932 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10868 same embryo
	EMAGE:10867 same embryo
	EMAGE:10866 same embryo
	EMAGE:10864 same embryo
	EurExpress:euxassay_003361 same experiment
	MGI:4823521 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source