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EMAGE:10882

Lsr lipolysis stimulated lipoprotein receptor ( MGI:1927471)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882
"Pseudo-wholemount" of euxassay_003312. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003312_01 euxassay_003312_02 euxassay_003312_03 euxassay_003312_04
EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882
euxassay_003312_05 euxassay_003312_06 euxassay_003312_07 euxassay_003312_08 euxassay_003312_09
EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882
euxassay_003312_10 euxassay_003312_11 euxassay_003312_12 euxassay_003312_13 euxassay_003312_14
EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882
euxassay_003312_15 euxassay_003312_16 euxassay_003312_17 euxassay_003312_18 euxassay_003312_19
EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882 EMAGE:10882
euxassay_003312_20 euxassay_003312_21 euxassay_003312_22 euxassay_003312_23 euxassay_003312_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10882Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10882_wholemount_strong.wlz
10882_wholemount_moderate.wlz
10882_wholemount_weak.wlz
10882_wholemount_possible.wlz
10882_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10882_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 12 13 14 15 16
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 18 19 20 moderate expression: see section 09 10 11 21
foregut-midgut junction
moderate moderate
regionalmoderate expression: see section 15 17 18
pancreas
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 17
thyroid gland
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 11 12 13
vibrissa
moderate moderate
regionalmoderate expression: see section 08 24 weak expression: see section 09 10 23
naris
moderate moderate
regionalmoderate expression: see section 14 15
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22
esophagus
moderate moderate
regionalmoderate expression: see section 13 14 15
stomach
strong strong
regionalstrong expression: see section 08 09 10 moderate expression: see section 03 04 05 06 07 11
hindgut
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 19 20
rectum
moderate moderate
regionalmoderate expression: see section 15 16 17
midgut
strong strong
regionalstrong expression: see section 08 09 10 moderate expression: see section 07 11 12 13 14 15 16 17 18 19 20 21
midgut loop
moderate moderate
regionalmoderate expression: see section 14 15 16 17
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 13 14 17 18 19 weak expression: see section 15 16
lower jaw molar
moderate moderate
regionalmoderate expression: see section 08 09 21
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 13 14 18 19 weak expression: see section 15
upper jaw molar
moderate moderate
regionalmoderate expression: see section 08 09 21
bladder
moderate moderate
regionalmoderate expression: see section 14 15 16
maturing glomerular tuft
weak weak
regionalweak expression: see section 09 10 11 12 19 20 21 22 23
testis
strong strong
regionalstrong expression: see section 23 24 moderate expression: see section 07 08 09 22
urethra of male
weak weak
regionalweak expression: see section 15 16 17
thyroid cartilage
moderate moderate
regionalmoderate expression: see section 12 13 14
left lung
strong strong
regionalstrong expression: see section 04 06 07 08 09 10 11 12 13 moderate expression: see section 05
right lung
strong strong
regionalstrong expression: see section 14 15 16 17 18 19 20 21 22 23 24
trachea
moderate moderate
regionalmoderate expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6244
Entity Detected:Lsr, lipolysis stimulated lipoprotein receptor ( MGI:1927471)
Sequence:sense strand is shown

>T6244
CGGACGCGTGGGCGCGGGCACCGTCGCTGCTAGACGGCCGCGATGGCGCCGGCGGCCAGCGCGTGTGCTG
GGGCGCCTGGCTCCCACCCGGCCACCACGATCTTCGTGTGTCTTTTTCTCATCATTTACTGCCCAGACCG
TGCCAGTGCCATCCAGGTGACCGTGCCTGACCCCTACCACGTAGTGATCCTGTTCCAGCCAGTGACACTA
CACTGCACCTACCAGATGAGCAATACCCTCACAGCCCCTATCGTGATCTGGAAGTATAAGTCGTTCTGTC
GGGACCGTGTTGCCGACGCCTTCTCCCCTGCCAGCGTGGACAACCAGCTCAACGCCCAGCTGGCGGCTGG
CAACCCCGGCTACAACCCCTATGTGGAGTGCCAGGACAGCGTACGCACTGTCAGGGTGGTGGCCACCAAA
CAGGGCAATGCTGTGACCCTGGGAGACTACTACCAGGGCAGGAGAATCACCATCACAGGAAATGCTGACC
TGACCTTCGAGCAGACGGCCTGGGGAGACAGTGGAGTGTATTACTGCTCCGTGGTCTCAGCCCAAGATCT
GGATGGGAACAACGAGGCGTACGCAGAGCTCATTGTCCTTGGCAGGACCTCAGAAGCCCCTGAGCTCCTA
CCTGGTTTTCGGGCGGGGCCCTTGGAAGATTGGCTCTTTGTGGTCGTGGTCTGCCTGGCAAGCCTCCTCT
TCTTCCTCCTCCTGGGCATCTGCTGGTGCCAGTGCTGTCCCCACACCTGCTGCTGCTATGTCAGATGTCC
CTGCTGCCCAGACAAGTGCTGTTGCCCTGAGGCCCTTTATGCTGCTGGCAAAGCAGCCACCTCAGGTGTG
CCAAGCATCTATGCCCCCAGCATCTATACCCACCTCTCTCCTGCCAAGACTCCGCCACCTCCGCCTGCCA
TGATTCCCATGCGTCCTCCCTATGGGTACCCTGGAGACTTTGACAGGACCAGCTCAGTTGGTGGCCACAG
CTCCCAGGTGCCCCTGCTGCGTGAAGTGGATGGGAGCGTATCTTCAGAAGTACGAAGTGGCTACAGGATC
CAGGCTAACCAGCAAGATGACTCCATGAGGGTCCTATACTATATGGAGAAGGAGCTAGCCAACTTCGATC
CTTCCCGGCCTGGCCCTCCCAATGGCCGAGTGGAACGGGCCATGAGTGAAGTAACCTCCCTCCATGAAGA
TGACTGGCGATCTCGGCCTTCCAGGGCTCCTGCCCTCACACCCATCAGGGATGAGGAGTGGAATCGCCAC
TCCCCTCGGAGTCCCAGAACATGGGAGCAGGAACCCCTTCAAGAACAGCCAAGGGGTGGTTGGGGGTCTG
GGCGGCCTCGGGCCCGCTCTGTGGATGCTCTAGATGACATCAACCGGCCTGGCTCCACTGAATCAGGAAG
GTCTTCTCCCCCAAGTAGTGGACGGAGAGGGCGGGCCTATGCACCTCCGAGAAGTCGCAGCCGGGATGAC
CTCTATGACCCCGACGATCCTAGAGACTTGCCACATTCCCGAGATCCCCACTATTATGATGATTTGAGGT
CTAGGGATCCACGTGCTGACCCCAGATCCCGTCAGCGATCCCACGATCCTCGGGATGCTGGCTTCAGGTC
ACGGGACCCTCAGTATGATGGGCGACTCTTAGAAGAGGCTTTAAAGAAAAAAGGGGCTGGGGAGAGAAGA
CGCGTTTACAGGGAGGAAGAAGAAGAAGAAGAGGAGGGCCACTATCCCCCAGCACCTCCGCCTTACTCTG
AGACTGACTCGCAGGCCTCGAGGGAGCGGAGGATGAAAAAGAATTTGGCCCTGAGTCGGGAAAGTTTAGT
CGTCTGATCCCACGTTTTGTTATGTAGCTTTTATACTTTTTTAATTGGAATATTGATGAAACTCTTCACC
AAGCCTAATAAAATTCCTGATCACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3500090 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3500090 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10879 same embryo
 EMAGE:10880 same embryo
 EMAGE:10881 same embryo
 EurExpress:euxassay_003312 same experiment
 MGI:4826016 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS