EMAGE mouse gene expression spatial database

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EMAGE:10892

Myo19 myosin XIX ( MGI:1913446)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003392. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003392_01	euxassay_003392_02	euxassay_003392_03	euxassay_003392_04

euxassay_003392_05	euxassay_003392_06	euxassay_003392_07	euxassay_003392_08	euxassay_003392_09

euxassay_003392_10	euxassay_003392_11	euxassay_003392_12	euxassay_003392_13	euxassay_003392_14

euxassay_003392_15	euxassay_003392_16	euxassay_003392_17	euxassay_003392_18	euxassay_003392_19

euxassay_003392_20	euxassay_003392_21	euxassay_003392_22	euxassay_003392_23	euxassay_003392_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10892_wholemount_strong.wlz

10892_wholemount_moderate.wlz

10892_wholemount_weak.wlz

10892_wholemount_possible.wlz

10892_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10892_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 05 06 07 09 10 11 moderate expression: see section 04 08

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 11 17 18 19 20 weak expression: see section 10

adenohypophysis

moderate

regional

moderate expression: see section 11 12 13 14 15 16

vibrissa

strong

regional

strong expression: see section 04 05 19 20 21

vomeronasal organ

strong

regional

strong expression: see section 04 05 06 07 08 11 14 17 18 19 20 21

hindgut

weak

regional

weak expression: see section 12 13 14 15 16 17 18

rectum

weak

regional

weak expression: see section 12

midgut

weak

regional

weak expression: see section 13 14 15 16 17 18

midgut loop

moderate

regional

moderate expression: see section 08 weak expression: see section 09 10 11 12

meckel's cartilage

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 17 18 19 20 21 22 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15 16

orbito-sphenoid

strong

regional

strong expression: see section 02 03 04 20 21 22 23 24 moderate expression: see section 01 05

clavicle

strong

regional

strong expression: see section 06 09 10 11 12 17 18 19 21 22 23 moderate expression: see section 07 08 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5989
Entity Detected:	Myo19, myosin XIX ( MGI:1913446)
Sequence:	sense strand is shown >T5989 GAAAACCCGTGTGGCCTGTCAGGCCTCCAGTGAGAGGAACTTTCACATCTTCTACCAGATTTGCAAAGGA GCCACCAAGGATGAAAGGCTCCAATGGCACCTCCCTGAGGGAACTGCCTTCTCCTGGCTACCCAATCCAG AAAGCAGTTTGGAAGAGGATTGTTTCGAGGTGACCAGAGAGGCCATGCTCCATTTGGGCATCGACACCCC CACCCAGAACAACATCTTTAAGGTCCTAGCTGGACTGCTGCACCTTGGCAATGTCCATTTTGTTGACTCA GAGGATGAAGCCCTGCCCTGCCAAGTGATGGATGATACCAAGGTCTCTGTGAGGACCTCAGCCTTGCTGC TGCAGCTCCCAGAGAAAATGCTGCTGGAGAGTATGCAGATTCGAACCATCAAGGCAGGCAAGCAGCAGCA GGTGTTCCAGAAGCCCTGCTCCCGAGCCTAGTGTGACACACGCAGAGACTGTCTGGCCAAACTGATCTAT GCACGGCTGTTCGACTGGCTGGTGTCTGTGATCAACAGCAGCATCTGTGCAGACTCCAAATCATGGACTG CCTTCATAGGGCTGCTAGATGTGTATGGGTTTGAGTCATTTCCTAATAACAGTTTGGAACAGCTGTGCAT CAACTATGCCAATGAGAAGCTACAGCAGCACTTCGTGGCTCACTACCTCAGGGCCCAGCAGGAGGAGTAC GAAGTTGAGGGCCTGGAGTGGTCATTTGTCAACTACCAGGACAACCAGACCTGCTTAGATCTCCTTGAAG GGAGCCCCATCAGCATCTGTTCCCTCATAAATGAGGAATGCCGCCTTAACCGGCCAAGCAGTGCAGCACA ACTACAGACGCGCATCGAGAGCACGCTGGCAGGACGGCCCTGCCTGGGCCATAACAAGCTCAGCCGGGAG CCCAGCTTCGTGGTTGTGCATTTCGCGGGACCTGTACGGTACCACACAGCCGGCCTGGTGGAAAAGAACA AGGACCCTGTTCCCCCTGAGCTGACTGAGCTCCTGCAGCAATCCCAAGACCCCCTGCTCACGATGCTGTT TCCTGCCAACCCTGAAGAGAAGACCCAGGAGGAGCTGTCTGGCCAGAGCCGGGCTCCTGCATTGACTGTG GTGTCCAAGTTCAAGGCCTCACTGGAACAGCTCCTGCAGGTCCTACATAATACAACACCCCACTACATTC GCTGCATCAAGCCCAACAGCCAGAGTCAGCCACAGACTTTCCTCCAAGAAGAGGTCCTGAACCAGTTAGA GGCCTGTGGCCTTGTGGAAACCATTCACATCAGTGCTGCTGGCTTTCCCATCCGGGTCTCTCACCAGAAC TTCATAGAAAGATATAAACTACTGAGAAGGCTCGGACCTCGCATGTCCTCTGGCCTCGGGGGCCTGGAGC CTGCCGAAGGGTCCTCTGAGCAGCCTCTGTGTGCCAAGGAGGCCACACTGCAACCTCTACTACAGGACAT TCTCCATGCTCTGCCAGCTTTAATTCAGACAGCAGCCACTCCCAGTGACCCGGCTAAGAACACACAAATC CCACTGTACTGTGGCAGGACAAAGATCTTCATGACTGACTCCATGCTAGAGCTTCTGGAATGTGGGCGTG CCCAGATGCTGGAGCAGTGTGCCCGTTGCATCCAGTGTGGCTGGAGGCGACACCGGCTCCAAAAGCAGGA GAAACAGAGGCGGGCTGCCGTGCTCATCCAGGCTGCTTTTAGGTCCTGGTTAACTCGGAAACACATCAGA AGGTTACACATAGCTGCCACGGTCATCAAGCACGCATGGCATAAGTGGAGAATCAGAATGGCCTGTCTTG CCTCTAAAGAACTGGATGGTATGGAGGAGAAACCCATGCCTCAGGCTCCTGGTACCCTGCGGTCCTCGAT GTCCCCAGCACACACTAGGTTCCTGGGAGCAATAATCCATCTCTGGCCCCTGGGACTGGTGCTAGCCAAC TCAGCTGATGGCGTACGTGGCTTTCAGAGGAAACTGGTAGCCCATGCCTGCCTTCGGCTTCCCTCAGACA GACCCAGCAACAAAGTCCAGACACCACAACAGGATCAGGCTGGTATTACATCTATCAGAGCACTGCCCCA GGGCTCGATAAAGTTTCACTGCAGGAAGTCTCCACTTCAGTATGCTGACATCTGCCCTGACCCTTCAGCC TCCTGTGTTACTGGCTTTAATCAGATTCTGCTAGAAAGCCACAGGCCAGTCCAAGTGTGACTCTTCTACC CCCACCTGCCTGAGTGATCTTTAGTGCCTTTGTTTCCACAAGGCCTTTTCCTGCCAGCTGTCTTGCCAAA GACATTAGATCCCACACGCAAGTGCCAAGCTATTCCCACGGCGACTCCAACTGCACGTGCAAGCAAATTC CAGCCTGAGCCAGGGCTTCATGGCCAGAGTGCCAGCCTTCCAGGGCTGTGGCTTCAGCACTGGCAGGATC TGGGGGTGCTGGGTACCAAGCCAGGATTAGGGCCACAACATCCCAGCTTAGTATCTTTGTGCTCAAGCTC CATCCAGAATGTTGTCACTACAGCAAGATGGATACTGGGCCATCCACAAGCACTTTAAAAGGGCCAAGTC ACCCTAAAATTGCCTCTAGAATTTTGGCTGGGGGAGAACTTGCATATAGTTTACTAAGGGAACAATAATA TTTAATAAACTGATTTATTTAATGTTACCAAGAACAATGAACAATGCAGCATCAAGAGAACCACACCTTA AATTGTTACACTATGTTTTTAATTCAAATGGATATACTTGGTATTTATGATGCAACTCTGAACTGTACAA GAAATAAAATTTAGAACCATCTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3486558 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3486558 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10894 same embryo
	EMAGE:10891 same embryo
	EMAGE:10893 same embryo
	EMAGE:10889 same embryo
	EMAGE:10890 same embryo
	EurExpress:euxassay_003392 same experiment
	MGI:4826559 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source