EMAGE mouse gene expression spatial database

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EMAGE:10926

BC002163 cDNA sequence BC002163 ( MGI:3612445)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003373. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003373_01	euxassay_003373_02	euxassay_003373_03	euxassay_003373_04

euxassay_003373_05	euxassay_003373_06	euxassay_003373_07	euxassay_003373_08	euxassay_003373_09

euxassay_003373_10	euxassay_003373_11	euxassay_003373_12	euxassay_003373_13	euxassay_003373_14

euxassay_003373_15	euxassay_003373_16	euxassay_003373_17	euxassay_003373_18	euxassay_003373_19

euxassay_003373_20	euxassay_003373_21	euxassay_003373_22	euxassay_003373_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10926_wholemount_strong.wlz

10926_wholemount_moderate.wlz

10926_wholemount_weak.wlz

10926_wholemount_possible.wlz

10926_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10926_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 07 08 09 22 23

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 10 11 17 18 weak expression: see section 12 15 16

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 15 16 weak expression: see section 08 09

pons mantle layer

moderate

regional

moderate expression: see section 07 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 20 weak expression: see section 05 06 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 07 08 09 17 18 19 20 weak expression: see section 05 06 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 17

ventral grey horn

moderate

regional

moderate expression: see section 09 10 11 12 13 weak expression: see section 14 15 not examined expression: see section 07

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 16 17

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5987
Entity Detected:	BC002163, cDNA sequence BC002163 ( MGI:3612445)
Sequence:	sense strand is shown >T5987 CCCACGCGTCCGAGCCGGCGGTCGGGAATTGCACCAGGGACCTGACAAGGGCACTGCAGAGCCATGCCTT TCCTTGACATACAGAAAAAGCTGGGCATCAGCCTGGACCGGCACTTTATGTTCCTAAGTGCAGAACAGCC CTACAAGAACGCCGCTCGGTGCCACGCGTTTGAAAAAGAGTGGATAGAGTGTGCACACGGGATCGGTGGG ACCCGGGCGAAAAAGGAGTGCAAGATAGAGTTCGATGACTTCGAAGAGTGCTTGCTTCGGTACAAAACGA TGAGGCGAATGCATGATATCAAGAAACAGCGGGAGAAGCTAATGAAAGAGGGCAAATACACCCCTCCACC TCACCATTCGGGCAGGGAGGAGCCCAGGCCCTGAGCGGGGCAGCTGGAGGCCGCTGTCATGCTCTGTTTT CCCCTGGAGAGAATATTTAAGGAAAGCTCCTTCATTAAGTATTAAGTATGTGGAAATAAAGAATTACTCA GTCTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3485964 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3485964 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10927 same embryo
	EMAGE:10930 same embryo
	EMAGE:10929 same embryo
	EMAGE:10925 same embryo
	EMAGE:10928 same embryo
	EurExpress:euxassay_003373 same experiment
	MGI:4823402 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source