EMAGE mouse gene expression spatial database

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EMAGE:10975

Tmem150a transmembrane protein 150A ( MGI:2385244)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001192. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001192_01	euxassay_001192_02	euxassay_001192_03	euxassay_001192_04

euxassay_001192_05	euxassay_001192_06	euxassay_001192_07	euxassay_001192_08	euxassay_001192_09

euxassay_001192_10	euxassay_001192_11	euxassay_001192_12	euxassay_001192_13	euxassay_001192_14

euxassay_001192_15	euxassay_001192_16	euxassay_001192_17	euxassay_001192_18	euxassay_001192_19

euxassay_001192_20	euxassay_001192_21	euxassay_001192_22	euxassay_001192_23	euxassay_001192_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10975_wholemount_strong.wlz

10975_wholemount_moderate.wlz

10975_wholemount_weak.wlz

10975_wholemount_possible.wlz

10975_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10975_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

weak

single cell

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4876
Entity Detected:	Tmem150a, transmembrane protein 150A ( MGI:2385244)
Sequence:	sense strand is shown >T4876 CGGACGCGTGGGCGAGGGGGCCGAGCAGGGAGAGGCGGCAGCGGCGGCGGGGAGCTCCGGGGAGGCCAGG GCAGCTGATGATTGTGACCGAAATATCTCAAAGTAGCTGGGCCTGCCCCCTTTTCCCCACCTACCTGTTG TCCTTACCCCCAAACTACCACCACCCTGGCTCCACTCCCTCATGACCGCCTGGATCCTCCTTCCTGTCAG CCTGTCAGCCTTCTCCATCACCGGCATATGGACTGTGTATGCCATGGCCGTGATGAACCGCCACGTGTGC CCAGTGGAGAACTGGTCCTACAATGAATCCTGCTCTCCTGACCCTGCTGAACAAGGGGGCCCTAAGAGCT GCTGCACCCTGGATGATGTTCCTCTCATCAGCAAGTGTGGCACATACCCCCCGGAGAGCTGCCTCTTCAG CCTCATTGGCAACATGGGTGCTGTTATGGTGGCCCTCATCTGCCTCCTTCGGTACGGGCAGCTCCTGGAA CAGAGCCGGCACTCCTGGATCAATACCACTGCACTCATCACTGGTTGCACCAACGCTGCAGGCCTCGTGG TGGTCGGCAATTTTCAGGTGGACCATGCCAAGTCTCTACACTACATCGGAACTGGTGTGGCCTTCACTGC TGGGCTGCTCTTTGTGTGCCTGCACTGTGTTCTCTTCTACCATGGGGCCACCACCCCCCTGGACATGGCT ATGGCCTACCTTCGAAGTGTGCTGGCTGTCATCGCCTTCATCACCCTGGTCCTTAGTGGAGTCTTCTTCC TCCACGAGAGTTCTCAGCTCCAGCATGGAGCTGCTCTGTGTGAATGGGTGTTTGTCCTCGATATCCTCAT TTTCTATGGCACCTTCAGCTATGAGTTCGGGACAATCTCCTCAGACACACTGGTGGCCGCACTACAGCCT GCCCCGGGCAGGGCCTGTAAGTCCTCTGGGAGCAGCAGCACCTCTACCCACCTCAACTGTGCTCCCGAGA GTATTGCCATGATCTGAGGTCTGGAGGGTGGCTGGCCCAGCCTCCAAAACACCCGCACCCTCTACCTTCT TTGCATTTATTTTGTACCAAAAACAAGTTTTAGAGAGTATTGTTGGGATCTAGGCTTCCTTACTTCTGGA GAAGTGGCCATCCAACACCCACCTGTGCCATAGAGGGACAGGGCCTCCACAGCTGCCTCTGCTGCTTGTC CTGTCCCCTCATACCAGAGTAGTATTCTGTGTTAAAGGTCACCTGTCCTCCCTTGCCCAGCCAGCCCTTC AGGTGCCTTCTACTCCCAGTGCCAGAGCCAGACCACTGGGGCCTTCTGCTGCAGGAATTGGGGCTGGGGT GGCAGGGGCTCACGAGTCTGGATGATGGGAGGAGCATGCCTTAGCCTAAGGGAAGGCCCACCCAGCTGCA CTTGGGTTCTTCACTCTGCCCCTCACTTCCTTTAGGGCAAATAACACAGCAGAACCACACGAGTATTTTA GTACTTTTTTATATCTATTAAAAGAATTCTAATTTGCACGTCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5097962 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5097962 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10976 same embryo
	EMAGE:10978 same embryo
	EMAGE:10979 same embryo
	EMAGE:10977 same embryo
	EMAGE:10980 same embryo
	EurExpress:euxassay_001192 same experiment
	MGI:4828779 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source