EMAGE mouse gene expression spatial database

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EMAGE:10996

Ngly1 N-glycanase 1 ( MGI:1913276)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001253. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001253_01	euxassay_001253_02	euxassay_001253_03	euxassay_001253_04

euxassay_001253_05	euxassay_001253_06	euxassay_001253_07	euxassay_001253_08	euxassay_001253_09

euxassay_001253_10	euxassay_001253_11	euxassay_001253_12	euxassay_001253_13	euxassay_001253_14

euxassay_001253_15	euxassay_001253_16	euxassay_001253_17	euxassay_001253_18	euxassay_001253_19

euxassay_001253_20	euxassay_001253_21	euxassay_001253_22	euxassay_001253_23	euxassay_001253_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10996_wholemount_strong.wlz

10996_wholemount_moderate.wlz

10996_wholemount_weak.wlz

10996_wholemount_possible.wlz

10996_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10996_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 03 04 05 06 07 19 20 21 22 23

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

vibrissa

weak

regional

weak expression: see section 09 10 11 12 13 23 24

hypothalamus mantle layer

weak

regional

weak expression: see section 11 12 13 14 15 16 17

telencephalon marginal layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

weak

regional

weak expression: see section 13 14 15 18 19 20 21

telencephalon ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate mantle layer

weak

regional

weak expression: see section 12

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 10 12 13 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 19 20

dorsal root ganglion

weak

regional

weak expression: see section 02

tail dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 11 12 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5404
Entity Detected:	Ngly1, N-glycanase 1 ( MGI:1913276)
Sequence:	sense strand is shown >T5404 CGCAGCCATGGCGTCGGCCACACTGGGCAGCTCGTCGAGCTCTGCGTCCCCGGCCGTGGCCGAGCTATGC CAGAACACCCCGGAGACCTTTCTGGAGGCCTCCAAGCTGCTGCTCACCTACGCCGACAACATCCTGAGAA ACCCCAGTGATGAAAAATACAGATCCATCCGTATTGGGAACACAGCGTTTTCTACTAGACTCTTGCCTGT CAGAGGAGCTGTTGAGTGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACGCATCTTATCTTTCCTAAA AAAGCTTCAGTGGAACAGCTACAGAAAATCCGTGATCTGATTGCCATAGAGAGAAGCAGCAGACTGGACG GATCCAGTAAGAAGGTGCAATTCTCTCAGCACCCGGCTGCTGCCAAGCTTCCTCTGGAGCAGTCTGAAGA TCCTGCTGGGTTAATACGGCACTCAGGAAACCAAACAGGGCAGCTGCCAAGCCTACCCTCTGCTCCAATG GTTGTTGGTGATTCAACCATCTTAAAAGTTCTTCAGTCCAACATTCAGCATGTGCAGCTCTATGAAAACC CTGTCCTCCAGGAGAAAGCATTGACTTGTATCCCAGTCAGTGAACTAAAAAGAAAAGCTCAAGAAAAGTT ATTCAGAGCTAGAAAGTTAGATAAAGGTACTAATGTAAGTGATGAGGACTTCCTTCTACTGGAGCTCCTA CACTGGTTTAAAGAGGAGTTTTTTCGCTGGGTGAATAACATTGTGTGCAGCAAATGTGGTGGAGAGACTA GATCTAGAGATGAGGCATTGCTGCCCAATGATGATGAACTGAAGTGGGGTGCAAAGAATGTGGAGAATCA TTACTGTGATGCCTGCCAGTTGAGCAACCGCTTCCCAAGATATAACAACCCCGAGAAACTTTTGGAAACA AGATGTGGACGCTGTGGTGAATGGGCCAATTGTTTTACTCTGTGCTGCCGTGCATTAGGGTTTGAAGCTC GATATGTGTGGGATTACACAGACCATGTTTGGACAGAAGTCTATTCTCCATCTCAGCAGCGGTGGCTACA CTGTGATGCATGTGAGGATGTCTGTGACAAACCCCTCCTTTATGAAATAGGATGGGGCAAGAAGCTTTCC TATATAATAGCATTCTCTAAAGATGAGGTAGTTGATGTCACCTGGCGATACTCTTGTAAACATGATGAGG TGATGTCCAGGAGGACCAAGGTTAAAGAAGAGTTACTTCGAGAAACTATTAATGGGCTTAATAAGCAGAG ACAGCTATCATTATCAGAAAGCAGAAGGAAAGAACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAATTT ATATCTCCTAAAACCCCAAGACCTGGAGAACTTGGTGGAAGGGTATCCGGGTCATTGGCTTGGAGAGTGG CCCGAGGTGAGACGGGTCTAGAGAGAAAGGAGATTCTGTTCATCCCTTCTGAAAATGAAAAGATTTCTAA GCAGTTTCACCTCCGTTATGATATTGTGAGAGACCGTTACATTCGTGTCTCAGACAACAACATAAACATT TCTGGATGGGAGAATGGTGTGTGGAAAATGGAATCCATATTCAGAAAAGTCGAGAAAGACTGGAACATGG TTTATTTGGCCCGAAAAGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAATGTGGGTCAGCTGG TCTAAAAGTAGATACTGTTTCCATCCGCACAAGTAGCCAAAGCTTTGAGTCTGGATCAGTGAGGTGGAAA CTGCGATCTGAGACAGCTCAAGTCAACCTGCTGGGAGATAAAAATCTACGTTCCTATAATGATTTTTCTG GTGCCACAGAGGTTACATTAGAAGCAGAGTTAAGCAGAGGAGATGGAGACGTTGCTTGGCAACATACCCA ACTGTTTAGACAAAGCTTAAATGACAGTGGAGAAAATGGCTTGGAAATAATTATAACGTTCAATGACCTC TGAAAGCCTGAACTTCAGGGAAATGCTGGCTACAATCACCGACCCGCCATGGCCTCCAGTAGTGTATTAG CTCAGTGTGTATCTAGTTGGCAGTTCACCATCCTGTGCAACAGGTTTCTTTTGCTGGCTGTACATCATGT AATCCTCATTAAAGTATATCTTCATAGTGTGGACCATAAGAAATCTTCAATTAAATGCTAACTTCTGCCT GTGATTAGAATTTGGCATAAAATATTTGGCATAAAGTAACTGTATGAAATTCTAATCTAAACACTGTTAC TAAAGTTGTAAGTGAAAATGATGGTATTCATGATTAAATGCTGTTCATGAATGTGATACAATCTAGGGCT ACTAGTTACTTAACATTTTCTGATTAGATTTTTCCAAACATTCTCAATTTTGAATAACTGTCAAAGTCAA GTATCTTATGTCATATGATGCATGAAGGTTTTTATCATTGTAAATATCTGGACAGTTAAAAAATAAAATG GATTATTTTTGCTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5366023 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5366023 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11000 same embryo
	EMAGE:10997 same embryo
	EMAGE:10999 same embryo
	EMAGE:10998 same embryo
	EMAGE:11001 same embryo
	EurExpress:euxassay_001253 same experiment
	MGI:4826701 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source